LC-ESI-MS/MS method for the simultaneous determination of isoformononetin, daidzein, and equol in rat plasma: Application to a preclinical pharmacokinetic study

Raju, K. Kanaka; Rashid, Mamunur; Gundeti, Manoj; Taneja, Isha; Malik, Monika; Singh, Sandeep K.; Chaturvedi, Swati; Challagundla, Muralikrishna; Singh, Sheelendra Pratap; Gayen, Jiaur R.; Wahajuddin, Muhammad

doi:10.1016/j.jchromb.2019.121776

Cited by 16 publications

(10 citation statements)

References 18 publications

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“…were phenylpropanoids, which were considered to be isoferulic acid, ferulic acid, p-coumaric acid, and (E)-4-Methoxycinnamic acid [30,31]. There were four flavonoid glycosides and five flavonoids, which were identified as quercetin-3β-d-glucoside (45) [34], cirsimarin (49) [35], astragalin (50) [36], kaempferol-7-O-glucoside (56) [37], desmethylxanthohumol (59) [38], isorhamnetin (61) [39], naringenin chalcone (63) [40], equol (65) [41], and galangin (82) [42], respectively.…”

Section: Phenolic Acid Derivativesmentioning

confidence: 99%

Rapid Characterizaiton of Chemical Constituents of the Tubers of Gymnadenia conopsea by UPLC–Orbitrap–MS/MS Analysis

et al. 2020

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Gymnadenia conopsea R. Br. is a traditional Tibetan medicinal plant that grows at altitudes above 3000 m, which is used to treat neurasthenia, asthma, coughs, and chronic hepatitis. However, a comprehensive configuration of the chemical profile of this plant has not been reported because of the complexity of its chemical constituents. In this study, a rapid and precise method based on ultra-high performance liquid chromatography (UPLC) combined with an Orbitrap mass spectrometer (UPLC–Orbitrap–MS/MS) was established in both positive- and negative-ion modes to rapidly identify various chemical components in the tubers of G. conopsea for the first time. Finally, a total of 91 compounds, including 17 succinic acid ester glycosides, 9 stilbenes, 6 phenanthrenes, 19 alkaloids, 11 terpenoids and steroids, 20 phenolic acid derivatives, and 9 others, were identified in the tubers of G. conopsea based on the accurate mass within 3 ppm error. Furthermore, many alkaloids, phenolic acid derivates, and terpenes were reported from G. conopsea for the first time. This rapid method provides an important scientific basis for further study on the cultivation, clinical application, and functional food of G. conopsea.

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Section: Phenolic Acid Derivativesmentioning

confidence: 99%

Rapid Characterizaiton of Chemical Constituents of the Tubers of Gymnadenia conopsea by UPLC–Orbitrap–MS/MS Analysis

et al. 2020

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“…The results of methodological investigation are shown in Table 1. Compounds 1 and 3 were definitely identified as daidzein (Raju et al, 2019) and taxifolin (Wang et al, 2009), which are isoflavones at m / z 253.0630 and 303.0634 [M − H] − . Based on their fragment ions and by comparison with the retention times, compound 15, 17, 20, 22, 27, 30, 35, 40 and 46 were identified as luteolin (Cheruvu et al, 2018), luteolin‐7‐O‐rutinoside (Yin et al, 2013), mauritianin (Yasukawa & Takido, 1987), rutin (Yang et al, 2012), apigenin‐7 ‐O‐ glucoside (Hvattum & Ekeberg, 2003), luteolin‐7 ‐O‐ glucoside (Ola et al, 2009), ligustroflavone (Pieroni, Pachaly, Huang, Van Poel, & Vlietinck, 2000), apigenin (Cheruvu et al, 2018) and quercetin (W. Yu et al, 2019), observed at m / z 285.0405, 593.1650, 739.2155, 609.1417, 739.2155, 431.1104, 447.0877, 723.2228, 269.0424, 301.03595 [M − H] − .…”

Section: Resultsmentioning

confidence: 99%

Analysis of chemical variations between raw and wine‐processed Ligustri Lucidi Fructus by ultra‐high‐performance liquid chromatography–Q‐Exactive Orbitrap/MS combined with multivariate statistical analysis approach

Zhang

Sun

Mao

et al. 2020

Biomedical Chromatography

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Ligustri Lucidi Fructus (LLF) is the dried and mature fruit of Ligubtrum lucidum Ait., which has the effect of nourishing the liver and kidney, brightening the eyes and promoting the growth of black hair. Wine‐processed LLF is commonly used in traditional Chinese medicine; however, the processing mechanisms are still unclear. Herein, a system data acquisition and mining strategy was designed to investigate the chemical profile differences between the raw and wine‐processed LLF, based on high‐performance liquid chromatography–Orbitrap high resolution mass spectrometry coupled with multivariate statistical analysis including principal component analysis and partial least square analysis. Afterwars, a total of 55 components were found to be the main contributors to the significant difference between raw and wine‐processed LLF by comparison with chromatographic behaviors, intact precursor ions, and characteristic MS fragmentation patterns. In addition, 10 main constituents of raw and wine‐processed LLF were simultaneously determined by UHPLC–MS/MS for analyzing the content variations. Some structural transformation mechanisms during wine processing were deduced from the results. The results may provide a scientific foundation for deeply elucidating the wine‐processing mechanism of LLF.

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“…For long time storage at − 80°C, daidzein, genistein, equol, and taxifolin in methanol were stable for 30 days. Another study [15] also found daidzein and equol were stable in methanol at − 70°C for 30 days. All storage conditions were stable because the accuracy and precision was ≤15%, within the acceptable limits.…”

Section: Stability Studymentioning

confidence: 86%

“…A variety of chromatography and detection techniques, for example, HPLC-UV, LC-UV/PDA, fluorescence, mass spectrometry (MS) or coularray electrode array detection [10][11][12], and gas chromatography with mass spectroscopy detection (GC-MS) [13] have been used for quantification of the aglycone forms. Nowadays, mass spectroscopy is a common detection technique because mass spectroscopy is very sensitive and specific [14][15][16][17][18][19][20]. However, sample preparation and sample cleanup before analysis by mass spectroscopy can be very complicated and time consuming; indeed previous studies using LC-MS/MS techniques for the quantification of total daidzein, genistein, and equol in human biological fluids required prolonged hydrolysis and cleanup by SPE [14,16,[21][22][23] and LLE [24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

A Simple and Rapid LC-MS/MS Method for Quantification of Total Daidzein, Genistein, and Equol in Human Urine

Saha

Kroon

2020

Journal of Analytical Methods in Chemistry

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Isoflavones and isoflavandiols have shown many health benefits, such as reducing cardiovascular disease, cancer, age-related disease, and osteoporosis. However, to investigate the relationships between consumption of isoflavones and their health benefits, it is important to be able to accurately quantify exposure in the large numbers of samples typically produced in association studies (i.e., several thousands). Current methods rely on solid-phase extraction protocols for sample cleanup, resulting in protracted extraction and analysis times. Here, we describe a fast and easy sample preparation method of human urine samples for subsequent quantification of daidzein, genistein (isoflavones), and equol (isoflavandiol) using LC-MS/MS. Sample preparation involves only the addition of dimethylformamide (DMF) and formic acid (FA) after enzymatic hydrolysis of their metabolites by a β-glucuronidase and sulfatase mixture. The method was validated by precision, linearity, accuracy, recoveries, limit of detection (LOD), and limit of quantification (LOQ). Linear calibration curves have been shown by daidzein, genistein, and equol. The correlation coefficients values are r2 > 0.99 for daidzein, genistein, and equol. LOD for daidzein and genistein was 1 ng/ml and equol was 2 ng/ml. Recoveries were >90%, and the relative standard deviation for intraday (<10%) and interday (≤20% over 10 days) was good. This method is suitable for quantification of isoflavones and the microbial metabolite equol in human urine and is particularly useful where large numbers of samples require analysis.

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LC-ESI-MS/MS method for the simultaneous determination of isoformononetin, daidzein, and equol in rat plasma: Application to a preclinical pharmacokinetic study

Cited by 16 publications

References 18 publications

Rapid Characterizaiton of Chemical Constituents of the Tubers of Gymnadenia conopsea by UPLC–Orbitrap–MS/MS Analysis

Rapid Characterizaiton of Chemical Constituents of the Tubers of Gymnadenia conopsea by UPLC–Orbitrap–MS/MS Analysis

Analysis of chemical variations between raw and wine‐processed Ligustri Lucidi Fructus by ultra‐high‐performance liquid chromatography–Q‐Exactive Orbitrap/MS combined with multivariate statistical analysis approach

A Simple and Rapid LC-MS/MS Method for Quantification of Total Daidzein, Genistein, and Equol in Human Urine

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