LC/ESI-MS/MS profiling of Ulmus parvifolia extracts and evaluation of its anti-inflammatory, cytotoxic, and antioxidant activities

Abstract

In this study, a comparative liquid chromatography/mass spectroscopy (LC/ESI-MS/MS) profiling of different fractions of Ulmus parvifolia leaves and stems was performed. Identification of compounds was based on comparing the mass spectrometric information obtained including m/z values and individual compound fragmentation pattern to tandem mass spectral library search and literature data. Eleven compounds were tentatively identified in the different analyzed fractions. One of the major constituents of this plant was isolated and identified as Icariside E4 [dihydro-dehydro-diconiferyl alcohol-4-O-α-L-rhamnopyranoside] (5). The evaluation of anti-inflammatory activity of the total methanolic extract using nitric oxide inhibition on LPS-stimulated RAW 264.7 cells model strong anti-inflammatory activity with 17.5% inhibition of nitric oxide production versus 10% inhibition for dexamethasone. The cytotoxic activity of the methanolic extract and Icariside E4 was evaluated against four types of human cell lines using MTT assay. Icariside E4 showed cytotoxic effect against Hep-G2, MCF-7, and CACO-2 cell lines compared to a negligible activity for the total extract. The same extract showed a moderate antioxidant activity with SC50=362.5 μg/mL.

Ulmus parvifolia Modulates Platelet Functions and Inhibits Thrombus Formation by Regulating Integrin αIIbβ3 and cAMP Signaling

“…Several Ulmus species reportedly possess anti-oxidative (Jung et al, 2010) and anti-platelet (Yang et al, 2013) properties. Ulmus parvifolia Jacq., commonly known as the Chinese Elm or Lacebark Elm, is native to Korea, Japan, and China, reportedly possesses anti-inflammatory (Mina et al, 2016), anti-allergic (Kim et al, 2016), anti-viral, and anticancer (Hamed et al, 2015) properties. While investigating newer and safer efficacious ethnomedicinal products, we recognized the medicinal properties of U. parvifolia bark.…”

Ulmus parvifolia Accelerates Skin Wound Healing by Regulating the Expression of MMPs and TGF-β

“…Bioactive components, such as sesquiterpenoids, triterpenoids, flavonoids, coumarins, and lignans, are mainly present in this species [16]. It has been reported that UP has analgesic and anti-inflammatory effects [12,14]; however, its role in skin wound healing has not been reported. Therefore, in the present study, we demonstrated for the first time the skin wound healing effect of the root bark of UP in SKH-1 hairless mice.…”

“…Recent studies on U. parvifolia Jacq. (UP), a species of elm native to China, Korea, and Japan, have shown that its leaves and stems have anti-inflammatory and antioxidant effects [12]. U. parvifolia bark, which contains phenolic compounds and steroidal glucosides, is used for the treatment of eczema and edema [13].…”

Ulmus Parvifolia Accelerate Skin Wound Healing by Regulating the Expression of MMPs and TGF-β

“…The bioactive components such as sesquiterpenoids, triterpenoids, flavonoids, coumarins, and lignans are mainly present in this species [15]. It has been reported that U. parvifolia have analgesic and anti-inflammatory effects [11,13]; however, its role in skin wound healing has not been reported. Therefore, in the present study, we have demonstrated for the first time the skin wound healing effect of the root bark of U. parvifolia in SKH-1 hairless mice.…”

“…Recent studies on U. parvifolia Jacq. (UP), a species of elm native to China, Korea, and Japan, have shown that its leaves and stems have anti-inflammatory and antioxidant effects [11]. U. parvifolia bark, which contains phenolic compounds and steroidal glucosides, is used for the treatment of eczema and edema [12].…”