LC-ESI-MS/MS quantification of carnosine, anserine, and balenine in meat samples

Uenoyama, Reiko; Miyazaki, Masao; Miyazaki, Tamako; Shigeno, Yuhei; Tokairin, Yoshinori; Konno, Hiroyuki; Yamashita, Tetsuro

doi:10.1016/j.jchromb.2019.121826

Cited by 24 publications

(15 citation statements)

References 21 publications

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“…The ANS content of ordinary muscle in skipjack tuna was higher than that in dark muscle. IDPs quantification results of the above five samples were consistent with previously reported data [11,[14][15][16]. Therefore, although the sample matrix effect needs to be considered individually, this method can be used to quantify the IDPs in various biological samples.…”

Section: Application To Other Biological Samplessupporting

confidence: 90%

“…To quantify IDPs, a methodology involving high-performance liquid chromatography (HPLC) separations with o-phthalaldehyde post-column derivatization has been reported [9][10][11][12]. Furthermore, a sensitive and specific analytical method using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) for IDP analysis has also been reported [13,14]. However, these methods require utilized equipment that may not be readily available in all laboratories, such as derivatization systems, fluorescence detector, and mass spectrometry.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of Histidine-Containing Dipeptides in Dolphin Serum Using a Reversed-Phase Ion-Pair High-Performance Liquid Chromatography Method

et al. 2021

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The quantification of histidine-containing dipeptides (anserine, carnosine, and balenine) in serum might be a diagnostic tool to assess the health condition of animals. In this study, an existing reversed-phase ion-pair high-performance liquid chromatography (HPLC)–ultraviolet detection method was improved and validated to quantify serum anserine, carnosine, and balenine levels in the dolphin. The serum was deproteinized with trichloroacetic acid and directly injected into the HPLC system. Chromatographic separation of the three histidine-containing dipeptides was achieved on a TSK–gel ODS-80Ts (4.6 mm × 150 mm, 5 µm) analytical column using a mobile phase of 50 mmol/L potassium dihydrogen phosphate (pH 3.4) containing 6 mmol/L 1-heptanesulfonic acid and acetonitrile (96:4). The standard curve ranged from 0.1 µmol/L to 250 µmol/L. The average accuracy of the intra- and inter-analysis of anserine, carnosine, and balenine was 97–106%. The relative standard deviations of total precision (RSDr) of anserine, carnosine, and balenine in dolphin serum were 5.9%, 4.1%, and 2.6%, respectively. The lower limit of quantification of these compounds was 0.11–0.21 µmol/L. These results indicate that the improved method is reliable and concise for the simultaneous determination of anserine, carnosine, and balenine in dolphin serum, and may be useful for evaluation of health conditions in dolphins. Furthermore, this method can also be applied to other biological samples.

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Section: Application To Other Biological Samplessupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Quantification of Histidine-Containing Dipeptides in Dolphin Serum Using a Reversed-Phase Ion-Pair High-Performance Liquid Chromatography Method

et al. 2021

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“…Another study shows the utility of HPLC with MS/MS based on the operation of a triple quadrupole (LC-ESI-MS/MS) for quality control of the species of meat or products by determining the presence of thermostable dipeptides (e.g., anserine, carnosine and balenin) [51].…”

Section: Column Liqiud Chromatography In Analysis Of Selected Bioactive Compounds In Food Samplesmentioning

confidence: 99%

Current Strategies for Studying the Natural and Synthetic Bioactive Compounds in Food by Chromatographic Separation Techniques

2021

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The present study summarizes the new strategies including advanced equipment and validation parameters of liquid and gas chromatography methods i.e., thin-layer chromatography (TLC), column liquid chromatography (CLC), and gas chromatography (GC) suitable for the identification and quantitative determination of different natural and synthetic bioactive compounds present in food and food products, which play an important role in human health, within the period of 2019–2021 (January). Full characteristic of some of these procedures with their validation parameters is discussed in this work. The present review confirms the vital role of HPLC methodology in combination with different detection modes i.e., HPLC-UV, HPLC-DAD, HPLC-MS, and HPLC-MS/MS for the determination of natural and synthetic bioactive molecules for different purposes i.e., to characterize the chemical composition of food as well as in the multi-residue analysis of pesticides, NSAIDs, antibiotics, steroids, and others in food and food products.

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“…In the past years scientific community has risen a great interest in carnosine studies, from its quantitation in food supplements to biological matrices. Therefore, several methods have been developed using high performance liquid chromatography coupled to either UV [8][9][10] or mass spectrometry detectors [11]. Moreover, methods relying on derivatization for improving either the retention time or the response (i.e.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of 13C labeled carnosine derivatives for isotope dilution mass spectrometry measurements in biological matrices

Maspero

Gilardoni

Bonfanti

et al. 2021

Talanta

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LC-ESI-MS/MS quantification of carnosine, anserine, and balenine in meat samples

Cited by 24 publications

References 21 publications

Quantification of Histidine-Containing Dipeptides in Dolphin Serum Using a Reversed-Phase Ion-Pair High-Performance Liquid Chromatography Method

Quantification of Histidine-Containing Dipeptides in Dolphin Serum Using a Reversed-Phase Ion-Pair High-Performance Liquid Chromatography Method

Current Strategies for Studying the Natural and Synthetic Bioactive Compounds in Food by Chromatographic Separation Techniques

Synthesis and characterization of 13C labeled carnosine derivatives for isotope dilution mass spectrometry measurements in biological matrices

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