LC–MS analysis of phospholipids and lysophospholipids in human bronchoalveolar lavage fluid

Barroso, Begoña; Bischoff, Rainer

doi:10.1016/j.jchromb.2004.07.044

Cited by 55 publications

(59 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among the various analytical methods, GC is merely used to analyze the variation of derivatized fatty acid chains of phospholipids [17,20], while HPLC could analyze the whole phospholipid molecules especially when coupled to MS/MS [21]. A large number of HPLC methods have been developed for the segregation analysis of phospholipids, which usually are carried out by columns packed with conventional HPLC particles (5 m), coupled with a binary mobile phase gradient of water and organic modifier (hexane, isopropanol, or acetonitrile) [22][23][24]. All of these approaches are equipped with detectors like UV, ELSD, MS, or MS/MS on low-resolution instruments (e.g.…”

Section: Introductionmentioning

confidence: 99%

Rapid identification of erythrocyte phospholipids in Sprague–Dawley rats by ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry

Yang

et al. 2015

J of Separation Science

View full text Add to dashboard Cite

A rapid, sensitive, and reliable approach for analyzing five kinds of erythrocyte phospholipids in Sprague-Dawley rats was provided by ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry with MassLynx(TM) MassFragment. Improving conventional high performance liquid chromatography techniques, ultra high performance liquid chromatography integrated with quadrupole time-of-flight tandem mass spectrometry offers high sensitivity and increased analytical speed by using columns packed with sub-2 μm particles (1.7 μm), which allows a faster separation to be achieved. Through this method, 83 phospholipids were tentatively characterized based on their mass spectra and tandem mass spectra, as well as by matching the in-house formula database within a mass error of 5 ppm, including 40 phosphatidylcholines, 24 phosphatidyl ethanolamines, three phosphatidylinositols, six phosphatidylserines, and ten sphingomyelins. Our present results proved that the established method could be used to qualitatively analyze complex erythrocyte phospholipids in Sprague-Dawley rats and provide a useful data base for pharmacology and phospholipidomics to seek potential biomarkers of disease prediction.

show abstract

Section: Introductionmentioning

confidence: 99%

Rapid identification of erythrocyte phospholipids in Sprague–Dawley rats by ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry

Yang

et al. 2015

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Recently, Barroso et al (2005), enhanced the RP-HPLC performance in the separation of PL in human bronchoalveolar lavage fluid. They used a C8 pellicular packing material at elevated temperature with an increasing gradient of acetonitrile containing 0.1% formic acid.…”

Section: Hplc Separationmentioning

confidence: 99%

Characterization of Phospholipid Molecular Species by Means of HPLC-Tandem Mass Spectrometry

Frega¹,

Pacetti²,

Boselli³

2012

Tandem Mass Spectrometry - Applications and Principles

View full text Add to dashboard Cite

“…The use of temperature programming, where the temperature is gradually ramped to account for varying volatilities of the sample, improves resolution and peak recovery (Myher and Kuksis, 1995). Barroso and Bischoff (2005) found that the use of a pellicular packing material in C 8 columns at high temperatures decreased excessive tailing and provided better resolution than normal phase. Using TLC or normal-phase HPLC to first separate the phospholipids by class will eliminate the issue of the molecular species of PE, PC and PI co-eluting (Pulfer and Murphy, 2003).…”

Section: Stationary Phasesmentioning

confidence: 99%

A review of chromatographic methods for the assessment of phospholipids in biological samples

Peterson

Cummings

2005

Biomedical Chromatography

236

159

View full text Add to dashboard Cite

Phospholipids are important constituents of all living cell membranes. Lipidomics is a rapidly growing field that provides insight as to how specific phospholipids play roles in normal physiological and disease states. There are many analytical methods available for the qualitative and quantitative determination of phospholipids. This review provides a summary of the methods that were historically used such as thin layer chromatography, gas chromatography and high-performance liquid chromatography. In addition, an introduction to applications of interfacing these traditional chromatographic techniques with mass spectrometry is provided.

show abstract

LC–MS analysis of phospholipids and lysophospholipids in human bronchoalveolar lavage fluid

Cited by 55 publications

References 30 publications

Rapid identification of erythrocyte phospholipids in Sprague–Dawley rats by ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry

Rapid identification of erythrocyte phospholipids in Sprague–Dawley rats by ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry

Characterization of Phospholipid Molecular Species by Means of HPLC-Tandem Mass Spectrometry

A review of chromatographic methods for the assessment of phospholipids in biological samples

Contact Info

Product

Resources

About