LC-MS application for therapeutic drug monitoring in alternative matrices

Avataneo, Valeria; D’Avolio, Antonio; Cusato, Jessica; Cantù, Marco; Nicolò, Amedeo De

doi:10.1016/j.jpba.2018.12.040

Cited by 75 publications

(68 citation statements)

References 167 publications

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“…The ME was calculated by comparing the signal from the analysis of post-extraction spiked samples (post-spiked) at high, medium and low QC levels with those from direct injection of the same concentration of analytes without matrix, as described by Taylor 17 and in FDA guidelines (post-extraction addition method). 14 The IS-nME effect was calculated as described by De Nicolò et al [18][19][20] Stability and impact of thermal inactivation…”

Section: Recovery (Rec) and Extraction Efficiency (Ee)mentioning

confidence: 99%

Development and validation of a UHPLC-MS/MS method for quantification of the prodrug remdesivir and its metabolite GS-441524: a tool for clinical pharmacokinetics of SARS-CoV-2/COVID-19 and Ebola virus disease

Avataneo

Nicolò

Cusato

et al. 2020

Journal of Antimicrobial Chemotherapy

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Background: Remdesivir has received significant attention for its potential application in the treatment of COVID-19, caused by SARS-CoV-2. Remdesivir has already been tested for Ebola virus disease treatment and found to have activity against SARS and MERS coronaviruses. The remdesivir core contains GS-441524, which interferes with RNA-dependent RNA polymerases alone. In non-human primates, following IV administration, remdesivir is rapidly distributed into PBMCs and converted within 2 h to the active nucleoside triphosphate form, while GS-441524 is detectable in plasma for up to 24 h. Nevertheless, remdesivir pharmacokinetics and pharmacodynamics in humans are still unexplored, highlighting the need for a precise analytical method for remdesivir and GS-441524 quantification.Objectives: The validation of a reliable UHPLC-MS/MS method for remdesivir and GS-441524 quantification in human plasma.Methods: Remdesivir and GS-441524 standards and quality controls were prepared in plasma from healthy donors. Sample preparation consisted of protein precipitation, followed by dilution and injection into the QSight 220 UHPLC-MS/MS system. Chromatographic separation was obtained through an Acquity HSS T3 1.8 lm, 2.1%50 mm column, with a gradient of water and acetonitrile with 0.05% formic acid. The method was validated using EMA and FDA guidelines.Results: Analyte stability has been evaluated and described in detail. The method successfully fulfilled the validation process and it was demonstrated that, when possible, sample thermal inactivation could be a good choice in order to improve biosafety.Conclusions: This method represents a useful tool for studying remdesivir and GS-441524 clinical pharmacokinetics, particularly during the current COVID-19 outbreak.

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Section: Recovery (Rec) and Extraction Efficiency (Ee)mentioning

confidence: 99%

Development and validation of a UHPLC-MS/MS method for quantification of the prodrug remdesivir and its metabolite GS-441524: a tool for clinical pharmacokinetics of SARS-CoV-2/COVID-19 and Ebola virus disease

Avataneo

Nicolò

Cusato

et al. 2020

Journal of Antimicrobial Chemotherapy

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“…LC–MS has been extensively used for sensitive and selective determination of trace level compounds in different complex matrices [1,2]. Particularly, LC–MS/MS with ESI ion source has been preferred in many research studies as lower temperatures compared to other ionization methods are used that are deemed suitable for thermally unstable compounds.…”

Section: Introductionmentioning

confidence: 99%

Determination of anabolic androgenic steroids as imidazole carbamate derivatives in human urine using liquid chromatography–tandem mass spectrometry

Fragkaki

Πετροπούλου

Αθανασιάδου

et al. 2020

J of Separation Science

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Anabolic androgenic steroids are widely abused substances in sports doping. Their detection present limitations regarding the use of soft ion sources such as electrospray or atmospheric pressure chemical ionization by liquid chromatography-tandem mass spectrometry. In the current study, a novel derivatization method was developed for the ionization enhancement of selected anabolic androgenic steroids. The proposed method aims at the introduction of an easily ionizable moiety into the steroid molecule by converting the hydroxyl groups into imidazole carbamates using 1,1 ′ -carbonyldiimidazole as derivatization reagent. The proposed method was applied to water and urine samples spiked with exogenous anabolic androgenic steroids in various concentration levels. Steroid imidazole carbamate derivatives have shown intensive [M+H] + signals under electrospray ionization and common fragmentation patterns in tandem mass spectrometry mode with [M-CO 2 +H] + and [M-ImCO 2 +H] + as major ions with low collision energy. The obtained results showed that the majority of steroids were detectable at concentrations equal or lower to their minimum required performance level according to the World Anti-Doping Agency technical document. The proposed method is sensitive with a preparation procedure that could be easily applied to the analysis of doping control samples. K E Y W O R D Sanabolic androgenic steroids, derivatization, doping control, imidazole carbamates, mass spectrometry

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“…Since its invention at the beginning of the 20th century, chromatographic method has undergone a whole century of development and is becoming the most important separation and analytical method in numerous fields. Thus far, chromatography has made a great contribution to bioanalysis, because of its outstanding separation efficiency, fast analysis speed, and high selectivity (Avataneo, D’Avolio, Cusato, Cantu, & De Nicolo, ; van den Ouweland & Kema, ).…”

Section: Introductionmentioning

confidence: 99%

Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

Chen

Gao

Zhong

2020

Biomedical Chromatography

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Chromatographic method has long been recognized as the most widely used separation method in bioanalytical research. However, the relatively low sensitivity of existing chromatographic methods remains a significant challenge, as the requirements for experimental procedures become more demanding. This review discusses the main causes for the low sensitivity of chromatographic methods and aims to introduce different technologies for enhancing their sensitivity in the following aspects: (i) different pretreatment methods for improving clean‐up efficiency and recovery; (ii) derivatization step for altering the chromatographic behavior of analytes and enhancing MS ionization efficiency; (iii) optimal LC–MS conditions and appropriate separation mechanism; and (iv) applications of other chromatographic methods, including miniaturized LC, 2D‐LC, 2D‐GC, and supercritical fluid chromatography. Altogether, this review is devoted to summarizing the recent technologies reported in the literature and providing new strategies for the detection of bioanalytes.

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LC-MS application for therapeutic drug monitoring in alternative matrices

Cited by 75 publications

References 167 publications

Development and validation of a UHPLC-MS/MS method for quantification of the prodrug remdesivir and its metabolite GS-441524: a tool for clinical pharmacokinetics of SARS-CoV-2/COVID-19 and Ebola virus disease

Development and validation of a UHPLC-MS/MS method for quantification of the prodrug remdesivir and its metabolite GS-441524: a tool for clinical pharmacokinetics of SARS-CoV-2/COVID-19 and Ebola virus disease

Determination of anabolic androgenic steroids as imidazole carbamate derivatives in human urine using liquid chromatography–tandem mass spectrometry

Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

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