LC-MS determination of steroidal glycosides from Dioscorea deltoidea Wall cell suspension culture: Optimization of pre-LC-MS procedure parameters by Latin Square design

Sarvin, Boris; Fedorova, E. S.; Шпигун, О. А.; Titova, M. V.; Никитин, М. А.; Кочкин, Д. В.; Родин, И. А.; Ставрианиди, А. Н.

doi:10.1016/j.jchromb.2018.02.012

Cited by 16 publications

(6 citation statements)

References 19 publications

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“…The main compounds found in D. deltoidea cell culture (over 4% of dry weight) were furostanol-type steroidal glycosides deltoside (protodeltonin), 25(S)-protodioscin (protoneodioscin) and protodioscin with diosgenin and yamogenin as aglycones (Table 3 and Figure 1b). Identification of these compounds was performed by using gas chromatography-mass spectrometry (GC-MS) and infrared spectroscopy of the products of complete acid hydrolysis of glycosides (aglycones) [21,35], comparison of the chromatographic behavior (HPLC-UV) of glycosides with a protodioscin standard [36], LC-ESI-MS analysis of individual glycosides [25,37] as well as by isolation of individual compounds from cell culture and their structural identification by 1 H-and 13 C-NMR spectroscopy, including two-dimensional NMR experiments 1 H-1 H COSY, TOCSY, NOESY, 1 H- 13 C HSQC and HMBC, and high-resolution mass spectrometry [30]. Figure 1b shows HPLC-ESI-MS chromatogram (positive-ion mode, selected ion monitoring (SIM) regime) for ions with m/z 1047.5 and 1031.5 [25] in the extract of the D. deltoidea cell culture grown in 630 L bioreactor.…”

Section: Cell Culture Growth In Bioreactors and Their Biochemical Compositionmentioning

confidence: 99%

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Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of Dioscorea deltoidea, Tribulus terrestris and Panax japonicus on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus

et al. 2021

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In the present study, we explored the therapeutic potential of bioreactor-grown cell cultures of the medicinal plant species Dioscorea deltoidea, Tribulus terrestris and Panax japonicus to treat carbohydrate metabolism disorders (CMDs) in laboratory rats. In the adrenaline model of hyperglycemia, aqueous suspensions of cell biomass pre-administered at a dose of 100 mg dry biomass/kg significantly reduced glucose level in animal blood 1–2.5 h (D. deltoidea and T. terrestris) or 1 h (P. japonicus) after adrenaline hydrochloride administration. In a streptozotocin-induced model of type 2 diabetes mellitus, the cell biomass of D. deltoidea and T. terrestris acted towards normalization of carbohydrate and lipid metabolism, as evidenced by a significant reduction of daily diuresis (by 39–57%), blood-glucose level (by 46–51%), blood content in urine (by 78–80%) and total cholesterol (25–36%) compared to animals without treatment. Bioactive secondary metabolites identified in the cell cultures and potentially responsible for their actions were deltoside, 25(S)-protodioscin and protodioscin in D. deltoidea; furostanol-type steroidal glycosides and quinic acid derivatives in T. terrestris; and ginsenosides and malonyl-ginsenosides in P. japonicus. These results evidenced for high potential of bioreactor-grown cell suspensions of these species for prevention and treatment of CMD, which requires further investigation.

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Section: Cell Culture Growth In Bioreactors and Their Biochemical Compositionmentioning

confidence: 99%

“…Identification of steroidal glycosides in biomass of D. deltoidea cell culture was performed by using gas chromatography-mass spectrometry (GC-MS), infrared spectroscopy (analysis of aglycones) [21,35], HPLC-UV [36], HPLC-ESI-MS [25,37] and NMR spectroscopy [30]. Quantitative analysis of steroidal glycosides was performed by using HPLC-ESI-MS [25].…”

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confidence: 99%

Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of Dioscorea deltoidea, Tribulus terrestris and Panax japonicus on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus

et al. 2021

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“…Some methods used to obtain the crude extract of plants and separate bioactive compounds are: supercritical fluid, maceration, hydrodistillation and ultrasound-assisted technique (Cruz et al, 2021;Ojha et al, 2020;FERREIRA et al, 2020;Sarvin et al, 2018;Bernardo et al, 2016;Boukroufa et al, 2015). Each method has different specifications regarding the extractive process, but all methods have characteristics that meet the principles of Green Chemistry, due to the use of solvents with lower environmental impact and/or in reduced volumes (Oliveira et al, 2016;Knez et al, 2014;Brasil, 2010).…”

Section: Resultsmentioning

confidence: 99%

“…Bioactive compounds, such as tannins, flavonoids, phenolics and trypsin inhibitors, have been identified (Cruz et al Research, Society and Development, v. 11, n. 6, e55211629315, 2022 (CC BY 4.0) Ngo et al, 2017;Almeida et al, 2014), including α-carotene, β-carotene, lutein, stigmasterol, α-cryptoxanthin, βcryptoxanthin andSitosterol (Cruz et al, 2021;Agostini-Costa et al, 2014). Some methods used to obtain the crude extract of plants and separate bioactive compounds are: supercritical fluid, maceration, hydrodistillation and ultrasound-assisted technique (Sarvin et al, 2018). Each method has different specifications regarding the extractive process, but all methods have characteristics that meet the principles of Green Chemistry, due to the use of solvents with lower environmental impact and/or in reduced volumes (Oliveira et al, 2016;Knez et al, 2014;Brasil, 2010).…”

Section: Introductionmentioning

confidence: 99%

Ora-pro-nobis - chemical characterization and sourcing of crude extract through different extraction methods: a review

Carnevalli

Ramos

Mardigan

et al. 2022

RSD

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Ora-pro-nobis has its center of origin in tropical America. It is found in Brazil, from the Northeast to Rio Grande do Sul. The plant belongs to the Cactaceae family, Pereskia genus. Its leaves and fruits contain bioactive compounds that may have expectorant properties and can be used to alleviate inflammatory processes in traditional medicine. Despite its richness, the plant is still little used by the general population and the food and pharmaceutical industries. Some extraction methods are used to obtain bioactive compounds from crude plant extracts, such as maceration, supercritical fluid, and ultrasound-assisted extraction, which have stood out for meeting the principles of green chemistry. The chemical characterization of the extracts obtained through chromatographic analysis by GC-MS/MS and LC-MS/MS proves to be extremely effective, as it allows quantifying the various chemical species present in the extract, making it possible to evaluate and compare the results generated in each chosen extraction condition. The objective of this research is to conduct a review study about the Pereskia aculeata Miller plant. To this end, an exploratory study was carried out, by means of theoretical surveys through Science direct, Capes, Scielo and Pubmed databases. Extracting from plants using different methods is an important step for the industry, ensuring improvement in the quality of products, especially in the food and pharmaceutical sectors. In this sense, no comparative study was found in the literature employing different extraction methods for ora-pro-nobis in the sense of identifying the best method, one with better yield and greater amount of bioactive compounds. The search for different extraction strategies integrated with processes and products that can extract intelligently, minimizing waste, shows an innovative potential in future research.

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“…For example, a recent study reported the optimized extraction of these compounds, with the highest extract content when 50% acetonitrile (ACN) was used for 60 minutes in ultrasonic extraction. However, compared with the yield of individual compounds, protodioscin had the best extraction efficiency at 50% ACN, whereas for dioscin, 70% ACN was more efficient [7]. Several analytical methods for determination of the above compounds have been reported previously.…”

Section: Introductionmentioning

confidence: 99%

Development of an Oriental Medicine Discrimination Method through Analysis of Steroidal Saponins in Dioscorea nipponica Makino and Their Anti-Osteosarcoma Effects

et al. 2019

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To prevent confusing Dioscorea nipponica (DN), an Oriental medicine, with Dioscorea quinquelobata (DQ) and Dioscorea septemloba (DS), a simple and accurate quantitative analysis method using HPLC combined with ultraviolet (UV) detection was developed and verified with UPLC-QTOF/MS through identification of five saponin glycosides: protodioscin (1), protogracillin (2), pseudoprotodioscin (3), dioscin (4), and gracillin (5). The newly developed analysis method showed sufficient reproducibility (<1.91%) and accuracy (92.1%–102.6%) and was able to identify DN based on the presence of compound 3 (13.821 ± 0.037 mg/mL) and the absence of 5. Compound 1, which is present in DN at a relatively high level (159.983 ± 0.064 mg/mL), was also an important marker for identification. Among the three species, DN showed the strongest activation of apoptotic signaling in osteosarcoma cells, while the four compounds detected in DN showed IC50 values of 6.43 (1), 10.61 (2), 10.48 (3), and 6.90 (4). In conclusion, the strong inhibitory effect of DN against osteosarcoma was confirmed to be associated with 1 and 4, which is also related to the quantitative results. Therefore, the results of this study might provide important information for quality control related to Oriental medicine.

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LC-MS determination of steroidal glycosides from Dioscorea deltoidea Wall cell suspension culture: Optimization of pre-LC-MS procedure parameters by Latin Square design

Cited by 16 publications

References 19 publications

Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of Dioscorea deltoidea, Tribulus terrestris and Panax japonicus on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus

Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of Dioscorea deltoidea, Tribulus terrestris and Panax japonicus on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus

Ora-pro-nobis - chemical characterization and sourcing of crude extract through different extraction methods: a review

Development of an Oriental Medicine Discrimination Method through Analysis of Steroidal Saponins in Dioscorea nipponica Makino and Their Anti-Osteosarcoma Effects

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