LC/MS/MS Analyses of an Oleander Extract for Cancer Treatment

Wang, Xiaomin; Plomley, Jeffry B.; Newman, Robert A.; Cisneros, Angela

doi:10.1021/ac991425a

Cited by 64 publications

(54 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The mass spectrometer data was collected by scanning from m/z 10 to 250 for 0.5 s/scan during the separation to characterize compounds. By infusing amino acid standards into the MS/MS system, quantitative optimizations for MRM [25][26][27] and for selective ion monitoring (SIM ) were automatically performed using the Analyst software. The optimized parameters, Q1 (protonated precursor ion), Q3 (product ion), declustering potential and collision energy for each amino acid, are listed in Table 1.…”

Section: Ce-esi-ms/ms Conditionsmentioning

confidence: 99%

Qualitative and quantitative analysis of amino acids by capillary electrophoresis‐electrospray ionization‐tandem mass spectrometry

et al. 2004

View full text Add to dashboard Cite

We describe a method to identify and quantify amino acids using capillary electrophoresis-electrospray ionization-triple-quadrupole tandem mass spectrometry (CE-ESI-MS/MS). Amino acids, including physiological amino acids, were first separated by CE under acidic pH conditions and then detected by MS/MS. To efficiently introduce the whole sample into the capillary, no electrical potential was applied to the electrospray probe until running electrophoresis. The position of the electrosprayer with respect to the MS capillary entrance drastically affected sensitivity and generation of cluster ions. MS/MS with multiple reaction monitoring (MRM) detection was performed to obtain sufficient selectivity and sensitivity. Under optimized CE-MS/MS conditions, the minimum detectable levels for 32 free amino acids normally found in proteins and other physiological amino acids were between 0.1 and 14 micromol/L with pressure injection of 50 mbar for 3 s (3 nL) at a signal-to-noise ratio of 3. For most amino acids, this constitutes a severalfold increase in sensitivity compared to CE-MS. The relative standard deviations (% RSD) for all amino acids were better than 0.4% for migration times and between 1.4% and 8.6% for peak areas (n = 10). Since amino acids exhibited characteristic MS/MS spectra, this approach is useful for the simultaneous, selective, quantitative, and reproducible analysis of amino acids in physiological and biological samples that contain various kinds of matrices. The power of the method was demonstrated by analyzing amino acids in human urine.

show abstract

Section: Ce-esi-ms/ms Conditionsmentioning

confidence: 99%

Qualitative and quantitative analysis of amino acids by capillary electrophoresis‐electrospray ionization‐tandem mass spectrometry

et al. 2004

View full text Add to dashboard Cite

show abstract

“…These include immunoassays using chemiluminescent (ACS), radio labelling (RIA), and fluorescence polarisation (FPIA) as detection system [5][6][7][8][9], as well as thin layer chromatography (TLC) [10,11] and high-performance liquid chromatography (HPLC) combined with derivatisation and fluorescence detection (FLD) [12,13] and with mass spectrometry (MS) [14][15][16][17], a very sensitive and specific method for the analysis of plant toxins [18,19].…”

Section: Introductionmentioning

confidence: 99%

A non-fatal oleander poisoning

et al. 2005

View full text Add to dashboard Cite

The study presents a case of non-fatal poisoning with oleander blooms in a 47-year-old female, with emphasis on the importance of toxicological service in a clinical emergency. After repeated vomiting at home, the patient was admitted at the hospital with cardiac symptoms more than 18 h after the ingestion. Serum samples were assayed immunochemically for digitoxin-related compounds by electrochemiluminescent immunoassay, and using HPLC/MS/MS analysis for oleandrin, the main cardiac glycoside of Nerium oleander. Confirming the non-specific immunoassay results, which are often clinically over-interpreted, oleandrin was detected by HPLC/MS/MS in the serum sample in a concentration of 1.6 ng/ml upon admission. Comparison with previous reports indicates that single compound analysis only permits a toxicological assessment for oleander poisoning and results in the proposal to classify an oleandrin level between 1.0 and 2.0 ng/ml as toxic blood plasma/serum concentration.

show abstract

“…Nerium oleander as important plant used the leaves and the flowers are cardiotonic, diaphoretic, diuretic, anticancer, antifungal antibacterial (Abe and Yamauchi, 1992;Wang et al 2000). This is a very poisonous plant, containing a powerful cardiac toxin and should only be used with extreme caution.…”

Section: Introductionmentioning

confidence: 99%

“…They used as purgative in dropsy and rheumatism. The whole plant is said to have anticancer properties (Abe and Yamauchi, 1992;Wang et al 2000;Zibbu and Batra 2010). For this purpose, we extraction of toxic components of N.oleanderspecialized steroids extraction The aim of this research is to investigate and examine the hepatic histologist structure alterations and biochemical parameters that may be caused by administration of Nerium oleander leaf extract to albino mice.…”

Section: Introductionmentioning

confidence: 99%