LC–MS–MS and GC–MS analyses of biologically active extracts of Tunisian Fenugreek (Trigonella foenum-graecum L.) Seeds

Keskes, Henda; Belhadj, Sahla; Jlail, Lobna; Feki, Abdelfattah El; Sayadi, Sami; Allouche, Noureddine

doi:10.1007/s11694-017-9632-0

Cited by 17 publications

(18 citation statements)

References 37 publications

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“…In another study, HPLC‐DAD‐ESI‐MS analysis of fenugreek seed methanolic extract, samples were injected into a C 18 column. Results showed the presence of 4‐hydroxyisoleucine, vicenin‐2, isoschaftoside, and isoorientin, the latter two compounds were reported for the first time in fenugreek seeds 46 …”

Section: Chromatographic Techniquesmentioning

confidence: 84%

“…In addition to δ ‐3‐carene, limonene, and cis ‐calamenene, which are terpenic compounds. Moreover, the study showed that β ‐thujone (10.8%), (2E,4E)‐decadienal (7.8%), heptanal (6.7%), and (2E,4E)‐heptadienal (5.3%) were reported for the first time in fenugreek seeds 46 …”

Section: Chromatographic Techniquesmentioning

confidence: 98%

“…showed the presence of 4-hydroxyisoleucine, vicenin-2, isoschaftoside, and isoorientin, the latter two compounds were reported for the first time in fenugreek seeds. 46 Banerjee et al used LC-MS/MS to investigate the hydroethanolic extract of fenugreek seeds. Trigonelline, luteolin, gallic acid, apigenin, calycosin, kaempferol, orientin, pratensin, quercetin, tricin, vicenin-2, vitexin, and fenugreekine were among the 13 bioavailable chemicals identified.…”

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confidence: 99%

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Advances in the quality control of fenugreek seeds using chromatographic, spectroscopic and DNA‐based techniques: A comprehensive review

Kandil

Ayoub

El-Ahmady

et al. 2021

Phytochemical Analysis

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Introduction Fenugreek has been used in traditional remedies since ancient times. It has a long history of use against medical ailments as an antidiabetic, anticarcinogenic, hypocholesterolemic, antioxidant, antibacterial, hypoglycemic, gastric stimulant, and anti‐anorexia agent. The major active constituents include alkaloids, fibres, saponins, proteins, and amino acids. Objectives To provide a comprehensive overview of the application of chromatographic and spectroscopic methods, in addition to DNA‐profiling methods to assess the quality of fenugreek. Also, to highlight the recent application of chemometrics combined with quality control methods during the last two decades. Methodology A literature search conducted from January 2000 up to December 2020 using various scientific databases (e.g., Scopus, Medline, PubMed, EBSCO, JSTOR, ScienceDirect, Google Scholar, Web of Science and Egyptian Knowledge Bank, Academic Journals, and Springer Link); general web searches were also undertaken using Google applying some related search terms. Studies involving the application of quality control analyses were classified into three categories according to the conducted analysis method including chromatographic [high‐performance liquid chromatography (HPLC), high‐performance thin‐layer chromatography (HPTLC), and gas chromatography (GC)], spectroscopic [ultraviolet (UV), infrared (IR), and nuclear magnetic resonance (NMR)], and DNA‐based markers. Results This review shed the light on relevant studies covering the past two decades, presenting the application of spectroscopic and chromatographic methods and DNA profiling in the quality control of fenugreek. Conclusion The reviewed chromatographic and spectroscopic methods combined with chemometrics provide a powerful tool that could be applied widely for the quality control of fenugreek.

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Section: Chromatographic Techniquesmentioning

confidence: 84%

Section: Chromatographic Techniquesmentioning

confidence: 98%

mentioning

confidence: 99%

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Advances in the quality control of fenugreek seeds using chromatographic, spectroscopic and DNA‐based techniques: A comprehensive review

Kandil

Ayoub

El-Ahmady

et al. 2021

Phytochemical Analysis

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“…Wadhwa et al. have used endogenous amino acid isoleucine as an IS (Table S1) [11]. Being an essential amino acid, the presence of isoleucine in plasma is well reported in the literature [13].…”

Section: Resultsmentioning

confidence: 99%

“…Keskes et al subjected the methanolic extracts of fenugreek seeds for HPLCcoupled diode array detector tandem ESI-LC-MS/MS analysis and studied the fragmentation pattern of 4-HIL (m/z 148 → 130, 102, 74) [10]. Recently reported LC-MS/MS method by Wadhwa et al has quantified 4-HIL from rat plasma [11]. Previously reported analytical methods for the identification or quantification of 4-HIL are summarized in Table S1.…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic interaction LC–MS/MS method to avoid endogenous interference in the analysis of 4‐hydroxy isoleucine from dietary supplementation of fenugreek

Gowtham

Das

Moksha

et al. 2022

J of Separation Science

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Hydroxy isoleucine is one of the potent hypoglycemic active constituents of fenugreek seeds. A method capable of reducing biological interferences is required for bioavailability studies. An isocratic separation of 4-hydroxy isoleucine from endogenous interferences was achieved in ZIC-cHILIC column using 0.1% formic acid in water and acetonitrile (20:80, % v/v) pumped at 0.5 ml/min. Quantification was performed in multiple reaction monitoring mode using the transitions of m/z 148.1→102.1 and m/z 276.1→142.2 for 4-hydroxy isoleucine and homatropine (as internal standard), respectively. After full method validation, 4-hydroxy isoleucine levels in human plasma and commercial fenugreek formulations were determined. This method showed good linearity in the range of 50-2000 ng/mL. Intra-and interday accuracies were in the range of 90.64-109.0% and precision was <4.82% CV. The mean (SD) plasma concentration of 4-hydroxy isoleucine in healthy individuals at 2 h after oral administration of fenugreek tablet was found to be 1590 (260) ng/mL. Half of marketed formulations were found to contain <0.05% of 4-hydroxy isoleucine content. We developed a rapid hydrophilic interaction liquid chromatography-tandem mass spectrometry method for analysis of 4-hydroxy isoleucine in human plasma. This method can be applied directly to conduct the clinical pharmacokinetics studies of 4-hydroxy isoleucine in human population.

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Identification of cyclooxygenase‐II inhibitory saponins from fenugreek wastes: Insights from liquid chromatography–tandem mass spectrometry metabolomics, molecular networking, and molecular docking

Khalil,

Sabry,

El‐Askary

et al. 2024

Phytochemical Analysis

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IntroductionThis research explores sustainable applications for waste generated from fenugreek (Trigonella foenum‐graecum), a plant with both nutritional and medicinal uses. The study specifically targets waste components as potential sources of nutrients and bioactive compounds.ObjectivesThe focus is to conduct detailed metabolic profiling of fenugreek waste, assess its anti‐inflammatory properties by studying its cyclooxygenase (COX) inhibitory effect, and correlate this effect to the metabolite fingerprint.Materials and MethodsEthanolic extracts of fenugreek fruit pericarp and a combination of leaves and stems were subjected to untargeted metabolic profiling using liquid chromatography–mass spectrometry integrated with online database searches and molecular networking as an effective dereplication strategy. The study also scrutinized the COX inhibitory capabilities of these extracts and saponin‐rich fractions prepared therefrom. Molecular docking was employed to investigate the specific interactions between the identified saponins and COX enzymes.ResultsThe analysis led to the annotation of 81 metabolites, among which saponins were predominant. The saponin‐rich fraction of the fruit pericarp extract displayed the strongest COX‐II inhibitory activity in the in vitro inhibition assay (IC50 value of 81.64 ± 3.98 μg/mL). The molecular docking study supported the selectivity of the identified saponins towards COX‐II. The two major identified saponins, namely, proto‐yamogenin 3‐O‐[deoxyhexosyl (1 → 2)] [hexosyl (1 → 4)] hexoside 26‐O‐hexoside and trigofenoside A, were predicted to have the highest affinity to the COX‐II receptor site.ConclusionIn the present study, we focused on the identification of COX‐II inhibitory saponins in fenugreek waste through an integrated approach. The findings offer valuable insights into potential anti‐inflammatory and cancer chemoprotective applications of fenugreek waste.

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LC–MS–MS and GC–MS analyses of biologically active extracts of Tunisian Fenugreek (Trigonella foenum-graecum L.) Seeds

Cited by 17 publications

References 37 publications

Advances in the quality control of fenugreek seeds using chromatographic, spectroscopic and DNA‐based techniques: A comprehensive review

Advances in the quality control of fenugreek seeds using chromatographic, spectroscopic and DNA‐based techniques: A comprehensive review

Hydrophilic interaction LC–MS/MS method to avoid endogenous interference in the analysis of 4‐hydroxy isoleucine from dietary supplementation of fenugreek

Identification of cyclooxygenase‐II inhibitory saponins from fenugreek wastes: Insights from liquid chromatography–tandem mass spectrometry metabolomics, molecular networking, and molecular docking

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