LC‐MS/MS determination and pharmacokinetic study of lacidipine in human plasma

Abstract

A robust, specific and fully validated LC-MS/MS method as per general practices of industry has been developed for estimation of lacidipine (LAC) with 100 μL of human plasma using lacidipine-(13) C8 as an internal standard (IS). The API-4000 LC-MS/MS was operated under the multiple reaction-monitoring mode. A simple liquid-liquid extraction process was used to extract LAC and IS from human plasma. The total run time was 3.0 min and the elution of LAC and IS occurred at 1.96 and 1.97 min; this was achieved with a mobile phase consisting of 5 mm ammonium acetate buffer-acetontrile (15:85 v/v) at a flow rate of 0.60 mL/min on a Zorbax SB C18 (50 × 4.6 mm, 5 µm) column. A linear response function was established for the range of concentrations 50-15,000 pg/mL (r > 0.998) for LAC. The current developed method has negligible matrix effect and is free from unwanted adducts and clusters which are formed owing to system such as solvent or mobile phase. The developed assay method was applied to an oral pharmacokinetic study in humans and successfully characterized the pharmacokinetic data up to 72 h.

Restricted access polypyrrole employed in pipette-tip solid-phase extraction for determination of nimodipine and nicardipine in breast milk

“…10,11 Table S5 † shows information from some studies involving CCBs in biological samples, such as analysis techniques, extraction techniques, type of sample, LOQ and LOD. [35][36][37][38][39][40][41] It is important to note that few studies employed SPE techniques, as for example PT-SPE reported in this work, with low sample and solvent consumption (mL instead of mL) and a small amount of material (20 mg). Despite some methods that use mass spectrometry detection having LOQ < 3 ng mL −1 , when compared to others that use UV detection, the LOQ reported in this work is the lowest ever found, demonstrating the innovation and applicability of this work that achieved recoveries around 100%.…”

Microwave Mediated Synthesis and Analytical Method Development for the Estimation of Novel 1,4-Dihydropyridines in Bulk by RP-HPLC

“…High therapeutic index of dihydropyridines has promoted the analytical method development for their assessment in bulk, dosage and in biological fluids. Literature study reveals that several analytical methods for the quantitative determination of dihydropyridines such as spectrophotometry [26,27], spectrofluorimetry [28], electrochemical methods [29] and HPLC-MS [30,31] have been reported. The inherent simplicity, reproducibility and highly selective nature of analysis make the liquid chromatography based estimations as the most suitable for quality control laboratories.…”

Simultaneous Estimation of Metoprolol Succinate and Lacidipine in Binary Combination Using High Performance Liquid Chromatographic Method = تقييم في وقت واحد للجمع بين ثنائي السكسينات ميتوبرولول و لاسيدبين باستخدام طريقة الكروماتوجرافي السائلة عالية الأداء

“…The active trans form is used in therapy (32)(33)(34)(35)(36) . Some analytical methods for the determination of lacidipine alone or/and combination with other drug in pharmaceutical dosage form and biological fluids have been reported, including some spectrophotometric method (37,38) , HPLC (39)(40)(41)(42)(43) , Stability-Indicating LC Method (44,45) high performance thin layer chromatography (HPTLC) (46) , LC-MS/MS methods (47)(48)(49)(50) , ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method (51) and SFC-MS/MS (52) . Monotherapy with various antihypertensive agents is not always sufficient to control the blood pressure and concomitant use of two or more drugs is necessary in 50% of the hypertensive patients (19) .…”