LC–MS Simultaneous Determination of Itopride Hydrochloride and Domperidone in Human Plasma

Abstract

A simple, rapid, cost-effective, and sensitive TLC-spectrodensitometric method for simultaneous determination of esomeprazole and domperidone was developed and tested in human plasma. Ethyl acetate: methanol: benzene: acetonitrile (5: 4: 8: 3, v/v/v/v) mobile phase was used for separation on TLC plates detected at 286 nm. The linearity ranges were 5-1200 and 2-600 ng/ spot for esomeprazole and domperidone, and limits of detection were 1.73 and 0.59 ng/spot. The effects of four variables affecting R f were evaluated by fractional factorial design. The benzene volume and saturation time had significant effects.

Development and validation of UPLC‐MS/MS method for determination of domperidone in human plasma and its pharmacokinetic application

“…A liquid chromatography coupled with tandem mass spectrometric (LC‐MS/MS) method providing an LLOQ of 0.189 ng/mL using an 800 μL plasma sample was used for the quantification of domperidone in human plasma (Smit et al ., ). Some other assays based on LC‐MS/MS (Bose et al ., ; Wu et al ., ; Zhang et al ., ) were also described for determining domperidone in human plasma. Xu et al .…”

Fourth‐derivative synchronous spectrofluorimetry and HPLC with fluorescence detection as two analytical techniques for the simultaneous determination of itopride and domperidone

“…Gastro‐esophageal reflux disease (GERD) is one of the most prevalent upper gastrointestinal disorders . A combination of itopride and domperidone performs better in GERD due to a blockade of the dopamine D2 receptor and inhibition of acetylcholine esterase .…”

“…Several methods have been reported for the determination of DOM including potentiometry , anodic differential pulse voltammetry , HPLC‐MS and HPLC with fluorescence detection . Only one analytical method has been reported for the simultaneous determination of ITP and DOM using HPLC with MS detection . However, the reported method requires expensive and sophisticated equipment that is not easily available.…”

A study of selective spectrophotometric methods for simultaneous determination of Itopride hydrochloride and Rabeprazole sodium binary mixture: Resolving sever overlapping spectra

“…Quite a lot of analytical methods were reported for the determination of Itopride hydrochloride in its pure form, pharmaceutical preparations and human plasma, including spectrophotometric methods [3][4][5], electrochemical method [6], TLC [7], liquid chromatography with ultraviolet detection [8][9][10][11][12], chemiluminescence detection [13] and fluorescence detection [14][15][16]. On the other hand, RB was analyzed by applying several methods like; spectrophotometric methods [17][18][19][20][21][22], chemometric method [23] and spectroflourimetric methods [24,25].…”