LC/MS: Theory, Instrumentation, and Applications to Small Molecules

Chen, Guodong; Zhang, Li‐Kang; Pramanik, Birendra N.

doi:10.1002/9780470087954.ch7

Cited by 13 publications

(17 citation statements)

References 108 publications

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“…is the redrawn fragmentation pathway for protonated florfenicol amine based on the published scheme. Similarly, florfenicol produced the ion at m/z 241, but the structure proposed by Chen et al for this product ion is incorrect because the radical cation is not a resonance structure as published . The previously proposed structure actually has an m/z value of 242, not the observed and reported m/z 241.…”

Section: Resultsmentioning

confidence: 90%

“…Chen et al . previously proposed a fragmentation pathway for florfenicol, and Fig. is the redrawn fragmentation pathway for protonated florfenicol amine based on the published scheme.…”

Section: Resultsmentioning

confidence: 99%

“…Product ion formation pathway for protonated florfenicol amine based on a previously published scheme for florfenicol …”

Section: Resultsmentioning

confidence: 99%

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Structural characterization of product ions by electrospray ionization and quadrupole time-of-flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods

Geis-Asteggiante

Núñez

Lehotay

2014

Rapid Commun. Mass Spectrom.

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Section: Resultsmentioning

confidence: 90%

“…Chen et al . previously proposed a fragmentation pathway for florfenicol, and Fig. is the redrawn fragmentation pathway for protonated florfenicol amine based on the published scheme.…”

Section: Resultsmentioning

confidence: 99%

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Structural characterization of product ions by electrospray ionization and quadrupole time-of-flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods

Geis-Asteggiante

Núñez

Lehotay

2014

Rapid Commun. Mass Spectrom.

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“…deprotonating) additives enhance ionization in an ESI source operating in negative ion mode [22]. All previous eluent selection strategies relied either on acidic (formic and acetic acids) [15,18] or basic (ammonium acetate) [23] additives favoring only separation or ionization, respectively.…”

Section: Resultsmentioning

confidence: 99%

An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

et al. 2012

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BackgroundPhytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and modulating plant reactions targeted against pathogens and herbivores, as well as resistance to abiotic stress (drought, UV-irradiation and mechanical wounding). These factors induce dramatic changes in phytohormone biosynthesis and transport leading to rapid local and systemic stress responses. Understanding of underlying mechanisms is of principle interest for scientists working in various areas of plant biology. However, highly sensitive, precise and high-throughput methods for quantification of these phytohormones in small samples of plant tissues are still missing.ResultsHere we present an LC-MS/MS method for fast and highly sensitive determination of jasmonates, abscisic and salicylic acids. A single-step sample preparation procedure based on mixed-mode solid phase extraction was efficiently combined with essential improvements in mobile phase composition yielding higher efficiency of chromatographic separation and MS-sensitivity. This strategy resulted in dramatic increase in overall sensitivity, allowing successful determination of phytohormones in small (less than 50 mg of fresh weight) tissue samples. The method was completely validated in terms of analyte recovery, sensitivity, linearity and precision. Additionally, it was cross-validated with a well-established GC-MS-based procedure and its applicability to a variety of plant species and organs was verified.ConclusionThe method can be applied for the analyses of target phytohormones in small tissue samples obtained from any plant species and/or plant part relying on any commercially available (even less sensitive) tandem mass spectrometry instrumentation.

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“…The purified fraction allows us to determine the mass of the compound as 479·2 g mol −1 . Noncovalent complexes such as protonated dimeric ions [2M + H] + and multimeric ions [3M + NH 4 ] + was reported as a common behaviour in LC/MS (Chen et al. 2007).…”

Section: Discussionmentioning

confidence: 99%

Production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136

Sánchez

Hedström

Delgado

et al. 2010

Journal of Applied Microbiology

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Aim: This study focuses on the production, purification and characterization of serraticin A, a novel cold‐active antimicrobial produced by Serratia proteamaculans 136. Methods and Results: A Ser. proteamaculans strain producing a novel cold‐active antimicrobial was isolated from Isla de los Estados, Argentina. Antimicrobial production was optimized in a BIOFLO 101 bioreactor under batch culture mode, with temperature, pH and dissolved oxygen controlled conditions. A purification protocol was developed including activated charcoal adsorption, solid‐phase C18 extraction (SPE) and semi‐preparative HPLC. The molecular weight was determined by LC/QTOF/MS/MS mass analysis. Conclusions: Serratia proteamaculans 136 produces a cold‐active low molecular bacteriocin‐like compound named serraticin A. In this work, it has been laboratory‐scale produced, purified and partially characterized. Cross‐immunity test revealed that serraticin A is very different from other well‐known microcins assayed, with a wide inhibitory spectrum, showing an interesting biotechnology potential to be applied as a control agent against pathogenic bacteria. Significance and Impact of the Study: The present study is the first report of a cold‐active compound with antimicrobial activity from Ser. proteamaculans. The work also highlights that cold environments could be a suitable source of micro‐organisms with ability to produce cold‐active biomolecules of biotechnological interest.

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LC/MS: Theory, Instrumentation, and Applications to Small Molecules

Cited by 13 publications

References 108 publications

Structural characterization of product ions by electrospray ionization and quadrupole time-of-flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods

Structural characterization of product ions by electrospray ionization and quadrupole time-of-flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods

An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

Production, purification and characterization of serraticin A, a novel cold-active antimicrobial produced by Serratia proteamaculans 136

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