LC–MS untargeted approach showed that methyl jasmonate application on Vitis labrusca L. grapes increases phenolics at subtropical Brazilian regions

Moro, Laís; Ros, Alessio Da; Mota, Renata Vieira da; Purgatto, Eduardo; Mattivi, Fulvio; Arapitsas, Panagiotis

doi:10.1007/s11306-020-1641-z

Cited by 18 publications

(39 citation statements)

References 36 publications

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“…However, it is important to highlight the changes in the quality and nutritional value of fruit ripening on the vine. Recent study showed the potential of methyl jasmonate pre-harvest application to increase several phenolic compounds in grapes, suggesting that this phytohormone can be used as elicitor to secondary metabolism [ 27 ]. In this context, methyl jasmonate has demonstrated the ability to stimulate the production of a wide variety of metabolites in tomatoes during ripening off-the vine.…”

Section: Resultsmentioning

confidence: 99%

Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits

Meza

Tobaruela

Pascoal

et al. 2022

Plants

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Methyl jasmonate hormone can stimulate the production of several metabolites responsible for improving fruit quality and nutritional attributes related to human health. In this context, efforts to manipulate tomatoes, such as using hormonal treatment to increase metabolite levels essential to plant growth and human nutrition, have received considerable attention. The aim of this study was to show the impact of metabolic profile on fruit quality and nutritional properties under exogenous methyl jasmonate during fruit ripening. The treatments were performed using 100 ppm of methyl jasmonate and 100 ppm of gaseous ethylene over 24 h. Ethylene emission, fruit surface color and metabolomics analysis were measured at 4, 10, and 21 days after harvest, considering the untreated fruits as control group. Methyl jasmonate induced the production of amino acids—mainly glutamine, glutamic acid and γ-aminobutyric acid (at least 14-fold higher)—and fatty acids—mainly oleic, linoleic, and linolenic acids (at least three-fold higher than untreated fruits); while exogenous ethylene predominantly affected sugar metabolism, increasing the levels of fructose, mannose and glucose to at least two-fold that levels in the untreated fruits. Additionally, methyl jasmonate significantly affected secondary metabolites, inducing by at least 80% the accumulation of α-tocopherol and β-sitosterol in fully ripe fruits. Our results suggest that the postharvest application of the hormone methyl jasmonate can contribute to the sensory characteristics and increase the nutritional value of the fruits since important changes related to the tomato metabolome were associated with compounds responsible for the fruit quality and health benefits.

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Section: Resultsmentioning

confidence: 99%

Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits

Meza

Tobaruela

Pascoal

et al. 2022

Plants

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“…To the best of our knowledge, up to September 2021, we were able to retrieve fourteen publicly available grapevine metabolome datasets [15,16,[32][33][34][35][36][37][38][39] and seven others related to wine science [40][41][42][43][44][45][46], with the majority of such studies deposited in the repository MetaboLights. It is foreseeable that the number will quickly increase in the near future as the application of metabolomics to plant science increases.…”

Section: Resultsmentioning

confidence: 99%

“…MTBLS784 [33,48] reported a study on the pre-harvest application of methyl jasmonate on Vitis labrusca grapes to verify the interaction with the phenolic compounds content by UPLC-HRMS-QTOF.…”

Section: Resultsmentioning

confidence: 99%

Grapevine and Wine Metabolomics-Based Guidelines for FAIR Data and Metadata Management

et al. 2021

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In the era of big and omics data, good organization, management, and description of experimental data are crucial for achieving high-quality datasets. This, in turn, is essential for the export of robust results, to publish reliable papers, make data more easily available, and unlock the huge potential of data reuse. Lately, more and more journals now require authors to share data and metadata according to the FAIR (Findable, Accessible, Interoperable, Reusable) principles. This work aims to provide a step-by-step guideline for the FAIR data and metadata management specific to grapevine and wine science. In detail, the guidelines include recommendations for the organization of data and metadata regarding (i) meaningful information on experimental design and phenotyping, (ii) sample collection, (iii) sample preparation, (iv) chemotype analysis, (v) data analysis (vi) metabolite annotation, and (vii) basic ontologies. We hope that these guidelines will be helpful for the grapevine and wine metabolomics community and that it will benefit from the true potential of data usage in creating new knowledge being revealed.

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“…DEGs involved in 12 secondary metabolism pathways under MeJA were studied, and indicated that an extensive transcriptional reprogramming of secondary metabolites biosynthesis was triggered by MeJA treatment ( Wang et al, 2017 ). Previous studies demonstrated that the application of MeJA induced the accumulation of different secondary metabolites in several plant species, including Lycoris chinensis ( Mu et al, 2009 ), Dendrobium officinale ( Jiao et al, 2018 ), Physalis angulata L. ( Zhan et al, 2020 ), Isatis tinctoria L. ( Gai et al, 2019 ), and Vitis labrusca L. ( Moro et al, 2020 ). One major regulatory mechanism of MeJA-mediated secondary metabolite production in many plants is through the control of pathway related genes ( Liu et al, 2018 ; Li et al, 2019a ; Li W. et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analyses Reveals That Exogenous Methyl Jasmonate Regulates Galanthamine Biosynthesis in Lycoris longituba Seedlings

Zheng

et al. 2021

Front. Plant Sci.

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The Amaryllidaceae alkaloid galanthamine (Gal) in Lycoris longituba is a secondary metabolite that has been used to treat Alzheimer’s disease. Plant secondary metabolism is affected by methyl jasmonate (MeJA) exposure, although the regulatory mechanisms of MeJA on L. longituba seedlings remains largely unknown. In the present study, 75, 150, and 300 μM MeJA were used as treatments on L. longituba seedlings for 7, 14, 21, and 28 days, while 0 μM MeJA was used as the control (MJ-0). The effect of exogenous MeJA on Gal synthesis in L. longituba was then investigated using transcriptomic sequencing and metabolite profiling via GC-MS and LC-MS analysis. Galanthamine (Gal), lycorine (Lyc), and lycoramine (Lycm) abundances were 2. 71-, 2. 01-, and 2.85-fold higher in 75 μM MeJA (MJ-75) treatment plants compared to MJ-0 treatment plants after 7 days of cultivation. Transcriptomic analysis further showed that MJ-75 treatment significantly induced the expression of norbelladine synthase (NBS) and norbelladine 4′-O-methyltransferase (OMT), which are involved in the Gal biosynthesis pathway. In addition, increased expression was observed in MJ-75 treatment plants for genes in the JA synthesis and JA signaling pathways including those of allene oxide cyclase (AOC), 12-oxo-phytodienoic acid reductase (OPR), jasmonic acid amino acid synthase (JAR), and transcription factor MYC. The L. longituba tyrosine decarboxylase (LlTYDC) enzyme was identified and proposed to be involved in the Gal biosynthetic pathway. Metabolomics results demonstrated that the accumulation of Amaryllidaceae alkaloids, and especially alkaloids in the Gal biosynthesis pathway, could be induced by MJ-75 treatment. Interestingly, metabolites in the JA synthesis pathway were also affected by MeJA treatment. Overall, this multi-omics study suggests that both the JA synthesis/JA signaling and Gal biosynthesis pathways were affected by exogenous MeJA treatment. This comprehensive study of gene expression and metabolite contents can help us better understand the molecular mechanisms underlying MeJA-mediated Gal biosynthesis in L. longituba.

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LC–MS untargeted approach showed that methyl jasmonate application on Vitis labrusca L. grapes increases phenolics at subtropical Brazilian regions

Cited by 18 publications

References 36 publications

Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits

Induction of Metabolic Changes in Amino Acid, Fatty Acid, Tocopherol, and Phytosterol Profiles by Exogenous Methyl Jasmonate Application in Tomato Fruits

Grapevine and Wine Metabolomics-Based Guidelines for FAIR Data and Metadata Management

Transcriptomic and Metabolomic Analyses Reveals That Exogenous Methyl Jasmonate Regulates Galanthamine Biosynthesis in Lycoris longituba Seedlings

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