LC–NMR, NMR, and LC–MS identification and LC–DAD quantification of flavonoids and ellagic acid derivatives in Drosera peltata

Braunberger, Christina; Zehl, Martin; Conrad, Jürgen; Fischer, Sophie; Adhami, Hamid-Reza; Beifuß, Uwe; Krenn, Liselotte

doi:10.1016/j.jchromb.2013.06.015

Cited by 50 publications

(37 citation statements)

References 14 publications

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“…S3 shows LC-MS/MS spectra of raw EA and m-EA freeze-dried powder. It can be seen that the molecular ion ([MÀ ÀH] À ) of the conjugates yields a MS 2 fragment at m/z 301.2 (Braunberger et al, 2013). EA has a Mr of 302 (Braunberger et al, 2013) and maltodextrin in m-EA freeze-dried powder was insoluble in methanol, so it can be inferred that this mass agrees with the published structure C 14 H 6 O 8 of EA.…”

Section: Ftir and Lc-ms/ms Analysissupporting

confidence: 54%

Preparation and characterization of micronized ellagic acid using antisolvent precipitation for oral delivery

Zhao

et al. 2015

International Journal of Pharmaceutics

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Section: Ftir and Lc-ms/ms Analysissupporting

confidence: 54%

Preparation and characterization of micronized ellagic acid using antisolvent precipitation for oral delivery

Zhao

et al. 2015

International Journal of Pharmaceutics

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“…(DAD) and photodiode array detector (PDA) are commonly used. Further detectors such as mass spectrometry (SM) and nuclear magnetic resonance (NMR) could be combined with UV formorecharacterizationofeachflavonoiddetected [32][33][34][35].…”

Section: Theadvantageofthistechniqueisalsoassociatedwithitsanalyticalmentioning

confidence: 99%

Isolation and Structure Characterization of Flavonoids

Awouafack¹,

Tane²,

Morita³

2017

Flavonoids - From Biosynthesis to Human Health

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Flavonoids are one of the most important classes of secondary metabolites from natural products due to their several applications in medicine, foods, diet industries, and so on. Even though a huge number has been reported from natural and synthetic sources, scientists are still interested in flavonoids and derivatives. The biggest challenge for working on secondary metabolites is related to the use of the predicted theoretical methodtoisolatetheexpectedcompoundandfinallyanalysethespectroscopicdatato elucidate and fully characterize the structure. This chapter was designed to document usefultechniquesforisolationandstructurecharacterizationofflavonoids.Besidesthe well-known methods that have been used so far, we would also put together updated information about novel challenge techniques published in recent articles on isolation andcharacterizationofflavonoids.Ourdatawereobtainedmainlyfromacademiclibrary and from reported data online by using research links such as Google Scholar, Scopus, SciFinder,Scirus,PubMed,andsoon.Ourfieldexperienceonphytochemistryofisolationandcharacterizationofflavonoidswasalsousedinthischapter.

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“…They provided the fragments at m/z 300.0 (deprotonated radical aglycone) and m/z 301.0 (aglycone) due to successive losses of glycoside fragments in MS 2 , and the ions at m/z 271 [M–glycosides–HCO] − , 255 [M–glycosides–C 2 H 5 O] − , 178.9, and 151.0 resulted from RDA fragmentation. The structures of these compounds were tentatively identified as rutin, isoquercitrin, and quercitrin .…”

Section: Resultsmentioning

confidence: 99%

HPLC–MS/MS determination of flavonoids in Gleditsiae Spina for its quality assessment

Jiang

Wang

et al. 2018

J of Separation Science

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Gleditsiae Spina, the thorn of Gleditsia sinensis Lam., has been used as an anti-inflammatory, anti-tumor, and anti-bacterial traditional medicine for hundreds of years in China. This study used high-performance liquid chromatography and tandem mass spectrometry combined with chemometric methods to allow the fast and accurate identification and quantification of the flavonoids compounds in Gleditsiae Spina, and created reliable criteria for accurate identification of Gleditsiae Spina and its adulterants. This research provides good evidence for the classification and quality evaluation of Gleditsiae Spina. Firstly, eight flavonoids compounds were detected and identified on the basis of their mass spectra, fragment characteristics, and comparison with published data. Then the mass spectroscopic fragmentation pathways of these compounds were determined and, in addition rutin, isoquercitrin, and quercitrin were detected in Gleditsiae Spina for the first time. The quantification was performed on a triple quadrupole tandem mass spectrometer in multi-reaction monitoring mode, and the baseline separation of the eight bioactive flavonoids components was achieved within 13 min. Furthermore, the proposed method was successfully applied for simultaneous quantitative determination of the eight Gleditsiae Spina compounds and adulterants obtained from different sources in China. Then, we built a classification model which showed a high level of accuracy predicting 100% of the samples, correctly.

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LC–NMR, NMR, and LC–MS identification and LC–DAD quantification of flavonoids and ellagic acid derivatives in Drosera peltata

Cited by 50 publications

References 14 publications

Preparation and characterization of micronized ellagic acid using antisolvent precipitation for oral delivery

Preparation and characterization of micronized ellagic acid using antisolvent precipitation for oral delivery

Isolation and Structure Characterization of Flavonoids

HPLC–MS/MS determination of flavonoids in Gleditsiae Spina for its quality assessment

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