LcERF19, an AP2/ERF transcription factor from <i>Litsea cubeba</i>, positively regulates geranial and neral biosynthesis

Wang, Minyan; Gao, Ming; Zhao, Yi; Chen, Yicun; Wu, Liwen; Yin, Hengfu; Yang, Jiahui; Xiong, Shifa; Wang, Siqi; Wang, Jue; Yang, Yang; Wang, Jia; Wang, Yangdong

doi:10.1093/hr/uhac093

Cited by 30 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genomic PCR using primers specific for both the Hygromycin gene and the CoDFR gene was performed to verify positive transformants. pCambia1300 empty vector was used as the control, and subcellular localization experiments were performed as described previously ( Wang et al., 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of dihydroflavonol 4-reductase (CoDFR) boosts flavonoid production involved in the anthracnose resistance

Yang

Cao

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

The outbreak of anthracnose caused by Colletotrichum spp. represents a devastating epidemic that severely affects oil tea (Camellia oleifera) production in China. However, the unknown resistance mechanism to anthracnose in C. oleifera has impeded the progress of breeding disease-resistant varieties. In this study, we investigated the physiological responses of resistant and susceptible lines during C. gloeosporioides infection. Our results showed that the accumulation of malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) in both disease-resistant and susceptible lines increased by C. gloeosporioides infection. Also, disease-resistant lines exhibited lower MDA, but higher POD, SOD, and CAT activities compared to susceptible lines. The accumulation of flavonoids in both resistant and susceptible C. oleifera leaves increased following C. gloeosporioides infection, and the increase was greater in resistant lines. Further, we identified and functionally characterized the dihydroflavonol 4-reductase (CoDFR) from the resistant C. oleifera line. We showed that the full-length coding sequence (CDS) of CoDFR is 1044 bp encoding 347 amino acids. The overexpression of CoDFR in tobacco altered the expression of flavonoid biosynthetic genes, resulting in an increased flavonoid content in leaves. CoDFR transgenic tobacco plants exhibited increased anthracnose resistance. Furthermore, the transgenic plants had higher salicylic acid content. These findings offer potential insights into the pivotal role of CoDFR involved in flavonoid-mediated defense mechanisms during anthracnose invasion in resistant C. oleifera.

show abstract

Section: Methodsmentioning

confidence: 99%

Overexpression of dihydroflavonol 4-reductase (CoDFR) boosts flavonoid production involved in the anthracnose resistance

Yang

Cao

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Because the stable transformation of L. cubeba is challenging, we used an efficient and simple transient expression assay to investigate the function of LcbHLH78 . We transiently overexpressed LcbHLH78 in L. cubeba following the transient transformation method of Wang M Y et al. (2022) .…”

Section: Resultsmentioning

confidence: 99%

Comprehensive identification of bHLH transcription factors in Litsea cubeba reveals candidate gene involved in the monoterpene biosynthesis pathway

et al. 2022

Self Cite

View full text Add to dashboard Cite

Litsea cubeba (Lour.) Person, an economically important aromatic plant producing essential oils, has lemon-like fragrance and 96.44–98.44% monoterpene contents. bHLH transcription factor plays an important role in plant secondary metabolism and terpene biosynthesis. In this study, we used bioinformatics to identify bHLH transcription factors in L. cubeba, 173 bHLH genes were identified from L. cubeba and divided these into 26 subfamilies based on phylogenetic analysis. The majority of bHLHs in each subfamily shared comparable structures and motifs. While LcbHLHs were unevenly distributed across 12 chromosomes, 10 tandem repeats were discovered. Expression profiles of bHLH genes in different tissues demonstrated that LcbHLH78 is a potential candidate gene for regulating monoterpene biosynthesis. LcbHLH78 and the terpene synthase LcTPS42 showed comparable expression patterns in various tissues and fruit development stages of L. cubeba. Subcellular localization analysis revealed that LcbHLH78 protein localizes to the nucleus, consistent with a transcription factor function. Importantly, transient overexpression of LcbHLH78 increased geraniol and linalol contents. Our research demonstrates that LcbHLH78 enhances terpenoid biosynthesis. This finding will be beneficial for improving the quality of L. cubeba and provides helpful insights for further research into the control mechanism of LcbHLH genes over terpenoid biosynthesis.

show abstract

“…Several TFs have been found to activate or repress terpenoid metabolism by binding to the promoter regions of TPSs or genes coding. LcERF19, an AP2/ERF transcription factor, could binding to LcTPS42 promoter and promoted its activity, which is related to the synthesis of geranial and neral in Litsea cubeba ( Wang et al., 2022a ). Belonging to the bHLH family, MYC2 has been shown to be involved in JA-regulated volatile aroma synthesis under stress in tea plant ( Li et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of QTL controlling volatile terpene contents in tea plant (Camellia sinensis) using a high-aroma ‘Huangdan’ x ‘Jinxuan’ F1 population

Chen

Liu

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Aroma is an important factor affecting the character and quality of tea. The improvement of aroma trait is a crucial research direction of tea plant breeding. Volatile terpenes, as the major contributors to the floral odors of tea products, also play critical roles in the defense responses of plants to multiple stresses. However, previous studies have largely focused on the aroma formation during the manufacture of tea or the comparison of raw tea samples. The mechanisms causing different aroma profiles between tea cultivars have remained underexplored. In the current study, a high-density genetic linkage map of tea plant was constructed based on an F1 population of ‘Huangdan’ × ‘Jinxuan’ using genotyping by sequencing. This linkage map covered 1754.57 cM and contained 15 linkage groups with a low inter-marker distance of 0.47 cM. A total of 42 QTLs associated with eight monoterpene contents and 12 QTLs associated with four sesquiterpenes contents were identified with the average PVE of 12.6% and 11.7% respectively. Furthermore, six candidate genes related to volatile terpene contents were found in QTL cluster on chromosome 5 by RNA-seq analysis. This work will enrich our understanding of the molecular mechanism of volatile terpene biosynthesis and provide a theoretical basis for tea plant breeding programs for aroma quality improvement.

show abstract

LcERF19, an AP2/ERF transcription factor from Litsea cubeba, positively regulates geranial and neral biosynthesis

Cited by 30 publications

References 36 publications

Overexpression of dihydroflavonol 4-reductase (CoDFR) boosts flavonoid production involved in the anthracnose resistance

Overexpression of dihydroflavonol 4-reductase (CoDFR) boosts flavonoid production involved in the anthracnose resistance

Comprehensive identification of bHLH transcription factors in Litsea cubeba reveals candidate gene involved in the monoterpene biosynthesis pathway

Identification of QTL controlling volatile terpene contents in tea plant (Camellia sinensis) using a high-aroma ‘Huangdan’ x ‘Jinxuan’ F1 population

Contact Info

Product

Resources

About