Lead Detection in Living Plant Tissue Using a New Histochemical Method

Glater, Ruth Ann B.; Hernandez, Louis

doi:10.1080/00022470.1972.10469663

Cited by 35 publications

(19 citation statements)

References 2 publications

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“…Chem. 15,1996 911 among the spruce samples with regard to internal lead was not as pronounced as with surface lead.…”

Section: Histochemical Detection Of Lead In Plant Tissuescontrasting

confidence: 46%

“…Although there was no direct proof confirming that the colored inclusions in rhodizonate-stained plant tissues were lead, this technique was well accepted and was used to study lead uptake in plants. Stained deposits of lead in cell walls of root tip tissues and in vascular conducting tissues of seedlings grown in high concentrations of lead were observed in various plant species such as corn, lettuce, radish, onion, pea, and beans [11,12,[15][16][17]. This staining technique confirmed that lead was transported apoplastically, but no lead deposits were observed inside protoplasm of living cells.…”

Section: Introductionmentioning

confidence: 58%

“…Chem. 15,1996 G. Tung and P.J. Temple sample collected 1,500 m from lead source contained markedly fewer stained areas.…”

Section: Application To Field-collected Plants Lead Contamination In mentioning

confidence: 97%

“…Only limited information on lead in stem and leaf tissues has been reported. Histochemical detection of lead was observed in the water-conducting vascular system and at the termini of watertranspiration pathways in leaves [11,14,15]. However, lead uptake and distribution in stems and leaves in plants are still poorly understood.…”

Section: Introductionmentioning

confidence: 97%

“…Glater and Hernandez [15] first introduced a histochemical staining technique using sodium rhodizonate to detect lead deposited in plant tissues. Although there was no direct proof confirming that the colored inclusions in rhodizonate-stained plant tissues were lead, this technique was well accepted and was used to study lead uptake in plants.…”

Section: Introductionmentioning

confidence: 99%

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Histochemical detection of lead in plant tissues

Tung

Temple

1996

Enviro Toxic and Chemistry

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Abstract-A histochemical staining technique using sodium rhodizonate was developed for detecting lead in living or preserved plant tissues. Sodium rhodizonate formed a bright scarlet-red precipitate with lead at pH 3.0, but showed no significant color responses with other metals. The precipitation of lead by this staining technique was confirmed by detection of lead in the red-stained precipitate with electron microscopy X-ray analysis. This histochemical technique for lead provided rapid, quantifiable, and unambiguous evidence for the accumulation and localization of lead in plant tissues. Soil-borne lead accumulated primarily in the roots, although at high concentrations, lead also accumulated at the ends of transpirational streams, particularly at hydathodes, trichomes, and the termini of xylem streams. Lead deposited from the atmosphere accumulated on the surface of conifer foliage and also appeared in or on cell walls of various internal cells and tissues. Lead concentrations in foliage and the color intensity of the stained deposits in spruce foliage decreased with distance from the lead source and increased with age of needles. No evidence of lead deposition inside cell contents was observed by this stain.

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“…Chem. 15,1996 911 among the spruce samples with regard to internal lead was not as pronounced as with surface lead.…”

Section: Histochemical Detection Of Lead In Plant Tissuescontrasting

confidence: 46%

Section: Introductionmentioning

confidence: 58%

“…Chem. 15,1996 G. Tung and P.J. Temple sample collected 1,500 m from lead source contained markedly fewer stained areas.…”

Section: Application To Field-collected Plants Lead Contamination In mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Histochemical detection of lead in plant tissues

Tung

Temple

1996

Enviro Toxic and Chemistry

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Use of rhodizonic acid for rapid detection of root border cell trapping of lead and reversal of trapping with DNase

2019

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Premise of the Study Lead (Pb) is a contaminant whose removal from soil remains a challenge. In a previous study, border cells released from root tips were found to trap Pb, alter its chemistry, and prevent root uptake. Rhodizonic acid ( RA ) is a forensic tool used to reveal gunshot residue, and also to detect Pb within plant tissues. Here we report preliminary observations to assess the potential application of RA in exploring the dynamics of Pb accumulation at the root tip surface. Methods and Results Corn root tips were immersed in Pb solution, stained with RA , and observed microscopically. Pb trapping by border cells was evident within minutes. The role of extracellular DNA was revealed when addition of nucleases resulted in dispersal of RA ‐stained Pb particles. Conclusions RA is an efficient tool to monitor Pb–root interactions. Trapping by border cells may control Pb levels and chemistry at the root tip surface. Understanding how plants influence Pb distribution in soil may facilitate its remediation.

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