Lead-Stimulated p38MAPK-Dependent Hsp27 Phosphorylation

Developmental exposure to manganese induces lasting motor and cognitive impairment in rats

“…To assess GFAP expression, western blot analysis of hippocampal and striatal samples was performed as previously described (Leal et al, 2002;Cordova et al, 2004;Rigon et al, 2008). Briefly, the structures were solubilized with 400 ml Tris 50 mM pH 7.0, EDTA 1 mM, NaF 100 mM, PMSF 0.1 mM, Na 3 VO 4 2 mM, Triton X-100 1%, glycerol 10%, sigma protease inhibitor cocktail and then incubated for 10 min at 4 8C.…”

Glutamate-induced Toxicity in Hippocampal Slices Involves Apoptotic Features and p38MAPK Signaling

“…All steps were followed by three times washing with TBS-T (0.05% Tween-20, 10 mM Tris, 150 mM NaCl, pH 7.5). Anti-cytochrome c (Oncogene, 1:500) and anti-caspase-3 (anti-32 and 17 kDa fragment) (Oncogene, 1:500) were used to detect specific proteins and the reactions were developed by ECL as previously described [22,42]. The optical density of bands was quantified by using the Scion Image software.…”

The influence of lead and arsenite on the inhibition of human breast cancer MCF-7 cell proliferation by American ginseng root (Panax quinquefolius L.)

“…There is evidence that arsenite induces genotoxicity and DNA damage (Schaumloffel and Gebel, 1998;Gebel et al, 1998), as well as apoptosis (de la Fuente et al, 2002;Hayakawa and Privalsky, 2004) in normal and malignant cells. Moreover, lead can induce cytotoxicity in a dose-dependent manner by altering the physical properties of cell membranes (Adonaylo and Oteiza, 1999), signal transduction pathways (Leal et al, 2002), and inducing DNA damage (McNeill et al, 2004) and cell growth arrest (Tchounwou et al, 2004). Interestingly, both arsenite and lead have also been shown to mimic the biological actions of estradiol in MCF-7 breast cancer cells maintained on charcoal-stripped serum (Martin et al, 2003).…”