Lead-stress induced changes in the content of free, thylakoid- and chromatin-bound polyamines, photosynthetic parameters and ultrastructure in greening barley leaves

Legocka, Jolanta; Sobieszczuk‐Nowicka, Ewa; Wojtyla, Łukasz; Samardakiewicz, Sławomir

doi:10.1016/j.jplph.2015.07.010

Cited by 30 publications

(12 citation statements)

References 63 publications

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“…acid-rain [49]. Conclusive evidence regarding antioxidant roles of PAs was provided by Nayyar and Chandler [50] and Legocka et al [45], who observed that exogenously applied Spm reduced H 2 O 2 and MDA contents in chickpea plants exposed to drought and cold stress and in greening barley leaves exposed to lead stress.…”

Section: Discussionmentioning

confidence: 97%

“…This seems to represent a general feature of plant responses to salinity [11,15]. Spm can be involved in stabilization of nucleic acid structure [44] and can operate as a free radical scavenger [45,46]. It was observed that Spm effectively prevented lipid peroxidation in senescing oats leaves [47], in sunflower leaf discs treated with paraquat [48], or in bean leaves treated with 6 Relationship between PA catabolism and Pro and GABA accumulation in the roots under salt stress.…”

Section: Discussionmentioning

confidence: 97%

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Putrescine catabolism via DAO contributes to proline and GABA accumulation in roots of lupine seedlings growing under salt stress

et al. 2017

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The levels of polyamines (PAs), proline (Pro), and γ-aminobutyric acid (GABA) as well as the activity of diamine oxidase (DAO; EC 1.4.3.6) were studied in the roots of 2-day-old lupine (Lupinus luteus L. 'Juno') seedlings treated with 200 mM NaCl for 24 h. The effect of adding 1 mM aminoguanidine (AG), an inhibitor of DAO activity, was also analyzed. It was found that in roots of lupine seedlings growing under salt stress, a negative correlation between Pro accumulation and putrescine (Put) content takes place. Pro level increased in roots by about 160% and, at the same time, Put content decreased by about 60%, as a result of ca. twofold increase of DAO activity. The AG added to the seedlings almost totally inhibited the activity of DAO, increased Put accumulation to control level, decreased Pro content by about 25%, and reduced GABA level by about 22%. Addition of 50 mM GABA to the lupine seedlings growing in the presence of AG and NaCl restored Pro content in roots to its level in NaCl-treated plants. In this research, the clear correlation between Put degradation and GABA and Pro accumulation was shown for the first time in the roots of seedlings growing under salt stress. This could be considered as a short-term response of a plant to high salt concentration. Our findings indicate that during intensive Pro accumulation in roots induced by salt stress, the pool of this amino acid is indirectly supported by GABA production as a result of Put degradation.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Putrescine catabolism via DAO contributes to proline and GABA accumulation in roots of lupine seedlings growing under salt stress

et al. 2017

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“…When studying dark-induced PA accumulation, it is important to note that induced PA accumulation at the beginning of the process could imply a ROS-scavenging function of both free and conjugated forms of PAs, as reported by Radyukina et al (2009) and Legocka et al (2015) . SD and SM, which have three and four amino groups, are more effective in the removal of reactive oxygen forms than PU ( Kuznetsov and Shevyakova, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

From Accumulation to Degradation: Reprogramming Polyamine Metabolism Facilitates Dark-Induced Senescence in Barley Leaf Cells

et al. 2016

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The aim of this study was to analyze whether polyamine (PA) metabolism is involved in dark-induced Hordeum vulgare L. ‘Nagrad’ leaf senescence. In the cell, the titer of PAs is relatively constant and is carefully controlled. Senescence-dependent increases in the titer of the free PAs putrescine, spermidine, and spermine occurred when the process was induced, accompanied by the formation of putrescine conjugates. The addition of the anti-senescing agent cytokinin, which delays senescence, to dark-incubated leaves slowed the senescence-dependent PA accumulation. A feature of the senescence process was initial accumulation of PAs at the beginning of the process and their subsequent decrease during the later stages. Indeed, the process was accompanied by both enhanced expression of PA biosynthesis and catabolism genes and an increase in the activity of enzymes involved in the two metabolic pathways. To confirm whether the capacity of the plant to control senescence might be linked to PA, chlorophyll fluorescence parameters, and leaf nitrogen status in senescing barley leaves were measured after PA catabolism inhibition and exogenously applied γ-aminobutyric acid (GABA). The results obtained by blocking putrescine oxidation showed that the senescence process was accelerated. However, when the inhibitor was applied together with GABA, senescence continued without disruption. On the other hand, inhibition of spermidine and spermine oxidation delayed the process. It could be concluded that in dark-induced leaf senescence, the initial accumulation of PAs leads to facilitating their catabolism. Putrescine supports senescence through GABA production and spermidine/spermine supports senescence-dependent degradation processes, is verified by H2O2 generation.

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“…Changes in the thylakoid structures, may cause a reduction in the chlorophyll and carotenoid content, triggering a diminished photosynthetic pigment biosynthesis, and chloroplasts functions (Aggarwal et al 2011;Cabrita et al 2016). Changes in the accumulation of polyamines, leading to free, thylakoid-and chromatin-bound polyamines in Pb-exposed barley leaves, have been shown to reduce photosynthetic pigments and photosynthetic parameters in barley leaves (Legocka et al 2015).…”

Section: Lancifoliusmentioning

confidence: 99%

The photosynthetic apparatus ofConocarpus lancifoliusEngl. (Combretaceae) suffers damage in soil contaminated with heavy metals

Redha

Al-Hasan

Jose

et al. 2019

Botany

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Conocarpus lancifolius Engl. (Combretaceae), a heat tolerant plant, has potential for use in the phytoremediation of polluted soil. We analyzed the physiological changes in C. lancifolius exposed to single and mixed heavy metals (HMs; cadmium, nickel, and lead). For 30 days under controlled growth conditions, we exposed some groups of plants to a single HM at concentrations of 25 or 50 μmol·L–1 and other groups were exposed to 25 μmol·L–1 of a mixture of HMs. Photosynthetic parameters such as electron transport rate, photosynthetic rate, chlorophyll fluorescence, chlorophyll content index, and photosynthetic pigments were measured. Chloroplast morphology was studied by transmission electron microscopy (TEM). In plants exposed to 25 μmol·L–1 of HMs, the photosynthetic parameters were unaffected, whereas at 50 μmol·L–1 of HMs, all parameters significantly decreased up to 20 days of exposure, followed by an increase up to 30 days, indicating a slow adaptation of plants to HM under stress. Compared with the single HMs, mixtures of HMs were more toxic at the same concentration. All parameters indicated damage to the photosynthetic apparatus due to stress from 25 μmol·L–1 of the HM mixtures and 50 μmol·L–1 of the single HMs. TEM analyses showed a dispersion of grana in the chloroplast of the affected C. lancifolius plants.

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Lead-stress induced changes in the content of free, thylakoid- and chromatin-bound polyamines, photosynthetic parameters and ultrastructure in greening barley leaves

Cited by 30 publications

References 63 publications

Putrescine catabolism via DAO contributes to proline and GABA accumulation in roots of lupine seedlings growing under salt stress

Putrescine catabolism via DAO contributes to proline and GABA accumulation in roots of lupine seedlings growing under salt stress

From Accumulation to Degradation: Reprogramming Polyamine Metabolism Facilitates Dark-Induced Senescence in Barley Leaf Cells

The photosynthetic apparatus ofConocarpus lancifoliusEngl. (Combretaceae) suffers damage in soil contaminated with heavy metals

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