Lead sulfide quantum dot–serum albumin bioconjugate: spectroscopic, microscopic investigation and photo-conducting current analysis

Mishra, A. K.; Bhunia, Amit Kumar; Saha, Suman

doi:10.1007/s00339-021-04483-z

Cited by 5 publications

(1 citation statement)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the recent past, nanoparticles (NPs), especially quantum dots (QDs), have become attractive research targets in chemistry, biology, and material science research owing to their fascinating properties such as size-tunable photoluminescence, longer lifetime, and high photoluminescence quantum yield. − Additionally, QDs are being extensively used in various biological applications − due to the development of optically bright, chemically stable, less-toxic, and highly photostable QDs. , The ability of QDs to bind with various biomolecules has made them quite interesting for various biological applications like biomarkers for fluorescence imaging, catalysis, nanomedicines, nanobiosensors, and NP-based molecular electronic circuits and bioinformatics. − These NP–protein bioconjugate systems also help in determining different biological responses like cellular uptake, circulation time, fibrillation, bioavailability, and even toxicity. ,− However, despite the availability of various methodologies to design and develop NPs/QDs with suitable functionalization, understanding the behavior of NPs, in particular QDs, in complex biological fluid still remains hazy.…”

Section: Introductionmentioning

confidence: 99%

Realization of a Model-Free Pathway for Quantum Dot–Protein Interaction Beyond Classical Protein Corona or Protein Complex

et al. 2022

View full text Add to dashboard Cite

Although in recent times nanoparticles (NPs) are being used in various biological applications, their mechanism of binding interactions still remains hazy. Usually, the binding mechanism is perceived to be mediated through either the protein corona (PC) or protein complex (PCx). Herein, we report that the nanoparticle (NP)−protein interaction can also proceed via a different pathway without forming the commonly observed PC or PCx. In the present study, the NP−protein interaction between less-toxic zinc−silver−indium-sulfide (ZAIS) quantum dots (QDs) and bovine serum albumin (BSA) was investigated by employing spectroscopic and microscopic techniques. Although the analyses of data obtained from fluorescence and thermodynamic studies do indicate the binding between QDs and BSA, they do not provide clear experimental evidence in favor of PC or PCx. Quite interestingly, highresolution transmission electron microscopy (HRTEM) studies have shown the formation of a new type of species where BSA protein molecules are adsorbed onto some portion of a QD surface rather than the entire surface. To the best of our knowledge, we believe that this is the first direct experimental evidence in favor of a model-free pathway for NP−protein interaction events. Thus, the outcome of the present study, through experimental evidence, clearly suggests that NP−protein interaction can proceed by following a pathway that is different from classical PC and PCx.

show abstract