Leaf-derived organogenesis in vitro for mass propagation of lisianthus (Eustoma grandiflorum (Raf.) Shinn

Winarto, Budi; Rachmawati, Fitri; Silva, Anggraeni

doi:10.9755/ejfa.2015.04.066

Cited by 6 publications

(9 citation statements)

References 10 publications

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“…Recent authors report similar survival rates of 90% (Winarto et al, 2015) for lisianthus 'White Lavender' or of 98% in the study conducted by Kaviani et al (2014).…”

Section: Length Of Shootssupporting

confidence: 58%

“…3). But other authors successfully used a mixture of burned-rice husk and organic manure 1:1 (Winarto et al, 2015) or peat and perlite 1:1 (Esizad et al, 2012) for acclimatization of lisianthus regenerated plantlets.…”

Section: Length Of Shootsmentioning

confidence: 99%

“…Eustoma grandiflorum is propagated by conventional methods: generative by seeds and vegetative by cuttings. Propagation by seeds shows a wide range of variation for certain traits due to their heterozygous character (Furukawa et al, 1990), also the germination rate is low at 34-39% (Winarto et al 2015). In vitro propagation can be effectively used for rapid multiplication and as explant are often used: stems, axillary buds, leaves and meristems in order to obtain homogenious material for increased high-quality yields in large scale production.…”

Section: Introductionmentioning

confidence: 99%

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In vitro plant propagation and crop improvement in Lisianthus (Lisianthus Russelianus Hook.)

et al. 2016

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Romania assists at the present time to an increase of production crops for ornamental plants and as a consequence an increased demand of planting material. Thus, improvements of the current multiplication methods are sought after. Lisianthus russelianus Hook. (Eustoma grandiflorum Grise.) is a relatively new floral crop to the international market, known for beautiful flowers of various colors and for having a long vase life. This study focused on the development of an efficient protocol for rapid regeneration of this species following known basic and applied aspects of lisianthus biotechnology but exploring new potentials. In the course of experiments conducted, for in vitro multiplication there were used nodal segments (1.5 cm) with axillary buds from three F 1 hybrids 'Echo Lavender', 'Flamenco White', 'Mirage Pastel Pink' that were inoculated on MS basal medium supplemented with 0.50 mg 1 -1 TDZ, 1.0 mg 1 -1 BAP and 0.50 mg 1 -1 AIA. The results show that the medium with BAP was most effective for obtaining the highest shoots number compared to medium containing TDZ. For rooting induction, two different concentrations of auxin IBA 0.5 mg 1 -1 and 1.5 mg 1 -1 were used simultaneously on MS basal medium. The highest roots number occurred when using 1.5 mg 1 -1 IBA. Both the number of shoots and rooting regeneration were dependent on the cultivar. The highest shoots number was achieved for 'Mirage Pastel Pink' hybrid (6.91) on the medium containing 1.0 mg 1 -1 BAP and 0.50 mg 1 -1 IAA.

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“…Recent authors report similar survival rates of 90% (Winarto et al, 2015) for lisianthus 'White Lavender' or of 98% in the study conducted by Kaviani et al (2014).…”

Section: Length Of Shootssupporting

confidence: 58%

Section: Length Of Shootsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro plant propagation and crop improvement in Lisianthus (Lisianthus Russelianus Hook.)

et al. 2016

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“…Convencionalmente se propaga vegetativamente y por semilla; sin embargo el método de propagación vegetativa es muy laborioso y tardado; más aún la propagación por semilla no es eficiente debido a la baja tasa de germinación de las mismas y la deficiente calidad de la progenie (Winarto et al, 2015). Gracias a las altas tasas de demanda del lisianthus, la micropropagación se hace una herramienta poderosa para la propagación a gran escala de plantas ornamentales como esta; ofreciendo la oportunidad de generar individuos vigorosos y libres de enfermedades en poco tiempo, los cuales serían difíciles de obtener por métodos de cultivo tradicionales (Clava & Pérez, 2005;Kaviani, 2014).…”

Section: Introductionunclassified

Organogénesis a partir de hojas de lisianthus (Eustoma grandiflorum) en respuesta a diferentes reguladores de crecimiento

Falcón-Bautista¹,

Rodríguez-Domínguez²,

Barba-González³

2018

mexjbiotechnol

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Lisianthus´ characteristics make it a cut flower of great interest, so the use of micropropagation techniques favors a rapid and massive multiplication, with plants free of pathogens. The organogenesis, resulted of use regulators of growth of different action mode (auxin, cytokinin and gibberellin), included the formation of shoots and roots that it was generated in some cases directly and in other from callus, employing the foliar segments as explant. The addition of low concentrations of cytokinin and gibberellin (1.0 mg/L benzyladenine (BA) + 0.5 mg/L gibberellic acid (GA3)) to the culture medium were necessary to generate the highest number of shoots (14.16 shoots per explant).

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“…Consequently, different media recipes with varying concentrations of various components such as carbohydrates (Akbari et al, 2014), vitamin and plant growth regulators (Kaviani, 2014;Winarto et al, 2015), hormones (Paek and Hahn, 2000), and solidifying agents (Semeniuk and Griesbach, 1987;Rezaee et al, 2012) as well as different concentrations of the same media mixture have been developed to propagate Eustoma from different plant parts. However, these previous studies have not addressed the timeframe for production or resulting plant quality, aspects that are important for commercial applications.…”

Section: Introductionmentioning

confidence: 99%

A timeline for Eustoma grandiflorum seedling production based on an in vitro germination protocol

Roni¹,

Islam²,

Shimasaki³

2017

Plant Omics

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Eustoma (Eustoma grandiflorum) is a high-rank ornamental cut flower, and propagation of plant material by seed is an important approach for high-volume production of Eustoma. Successful propagation using different parts of the Eustoma plant has been reported in numerous studies; however, to date there have been no studies comparing the timeline differences between in vitro and conventional germination growth methods. The objective of this study was to develop a convenient and straightforward in vitro protocol that decreases the lag time to germination and the seedling development periods. Seed germination and seedling growth was studied in Eustoma (Voyage type 2 pink) seeds using two different techniques, in vitro germination and conventional sowing in soil. Seeds sown under in vitro conditions exhibited faster (6 days) germination compared with seeds sown directly in soil (11 days). Halfstrength Murashige and Skoog medium in vitro-grown seedlings (7 weeks) were more vigorous and produced true leaves earlier compared with conventionally-grown seedlings (15 weeks). In addition, when Eustoma seeds were sown in vitro, germination could be more easily tracked and visually compared with seeds sown in soil media. Therefore, the in vitro seed germination protocol developed in this study is straightforward, reproducible, and will amenable to facilitate efficient seedling production of Eustoma for commercial applications.

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Leaf-derived organogenesis in vitro for mass propagation of lisianthus (Eustoma grandiflorum (Raf.) Shinn

Cited by 6 publications

References 10 publications

In vitro plant propagation and crop improvement in Lisianthus (Lisianthus Russelianus Hook.)

In vitro plant propagation and crop improvement in Lisianthus (Lisianthus Russelianus Hook.)

Organogénesis a partir de hojas de lisianthus (Eustoma grandiflorum) en respuesta a diferentes reguladores de crecimiento

A timeline for Eustoma grandiflorum seedling production based on an in vitro germination protocol

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