Leaf histochemistry analysis of four medicinal species from Cerrado

Kuster, Vinícius Coelho; Vale, Fernando Henrique Aguiar

doi:10.1016/j.bjp.2016.05.015

Cited by 18 publications

(17 citation statements)

References 33 publications

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“…Na fração de acetato de etila foram isolados 5,7,3, 4-tetrahidroxilflavan-3-ol (catequina) e quercetina-3-O-ramnosídeo (quercitrina) (Madalosso et al, 2012). Kuster não detectou flavonoides em folhas de C. adamantium (Kuster, Vale, 2016).…”

Section: Resultsunclassified

Flavonoides presentes nos extratos da campomanesia xanthocarpa Berg

Dalastra

Southier

Anaissi

et al. 2019

BJD

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Section: Resultsunclassified

Flavonoides presentes nos extratos da campomanesia xanthocarpa Berg

Dalastra

Southier

Anaissi

et al. 2019

BJD

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“…A histological analysis was performed to investigate the tissue structure of the in vitro shoots, and a histochemical analysis with Nadi was carried out to identify secretory structures storing terpenoids. Nadi staining has been described as a tool to detect terpenoids [ 63 ] and has been used to this effect in a number of studies [ 64 , 65 ]. The method is a reaction that is believed to result from the oxidation of naphthalen-1-ol and N,N-dimethylbenzene-1,4-diamine by cytochrome oxidase enzymes [ 66 ].…”

Section: Methodsmentioning

confidence: 99%

Use of a temporary immersion bioreactor system for the sustainable production of thapsigargin in shoot cultures of Thapsia garganica

et al. 2018

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BackgroundThapsigargin and nortrilobolide are sesquiterpene lactones found in the Mediterranean plant Thapsia garganica L. Thapsigargin is a potent inhibitor of the sarco/endoplasmic reticulum calcium ATPase pump, inducing apoptosis in mammalian cells. This mechanism has been used to develop a thapsigargin-based cancer drug first by GenSpera and later Inspyr Therapeutics (Westlake Village, California). However, a stable production of thapsigargin is not established.ResultsIn vitro regeneration from leaf explants, shoot multiplication and rooting of T. garganica was obtained along with the production of thapsigargins in temporary immersion bioreactors (TIBs). Thapsigargin production was enhanced using reduced nutrient supply in combination with methyl jasmonate elicitation treatments. Shoots grown in vitro were able to produce 0.34% and 2.1% dry weight of thapsigargin and nortrilobolide, respectively, while leaves and stems of wild T. garganica plants contain only between 0.1 and 0.5% of thapsigargin and below detectable levels of nortrilobolide. In addition, a real-time reverse transcription PCR (qRT-PCR) study was performed to study the regulatory role of the biosynthetic genes HMG-CoA reductase (HMGR), farnesyl diphosphate synthase (FPPS), epikunzeaol synthase (TgTPS2) and the cytochrome P450 (TgCYP76AE2) of stem, leaf and callus tissues. Nadi staining showed that the thapsigargins are located in secretory ducts within these tissues.ConclusionsShoot regeneration, rooting and biomass growth from leaf explants of T. garganica were achieved, together with a high yield in vitro production of thapsigargin in TIBs.Electronic supplementary materialThe online version of this article (10.1186/s13007-018-0346-z) contains supplementary material, which is available to authorized users.

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“…The positive control was showed as suggested by the respective authors of histochemical tests. Test for alkaloids, phenols and proteins were done according to the procedure of Kuster and Vale [17]. Temporary mount sections were observed under the compound microscope.…”

Section: Histochemical Studiesmentioning

confidence: 99%

“…This method has been developed for qualitative and quantitative analysis for the cellular components, including proteins, carbohydrates, lipids, nucleic acids and the range of ionic elements occurring in the cells [23]. Through histochemical tests, it was possible to identify the region of synthesis and storage of metabolites in tissue level for pharmacological uses in leaves, stem and root of medicinal plants [17].…”

Section: Histochemical Studiesmentioning

confidence: 99%

Histochemical and Phytochemical Analysis of Medicinally Important Plants

Sushma

Ashalatha

Ray

et al. 2020

EJMP

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The present study was aimed to investigate the histochemical and phytochemical studies of four important medicinal plants. Leaf, stem and root sections were examined for alkaloid, protein and phenolic tests. Methanolic extracts of leaf, stem and root were analysed for the phytochemical screening of major bioactive constituents. Histochemical analysis reveals the presence of alkaloids, phenols and proteins in epidermis, cortex and vascular bundles of root, stem and leaf. Preliminary phytochemical analysis showed the presence of alkaloids, tannins, phenols, flavonoids, steroids, saponins, terpenoids, coumarins, quinone and proteins. The root extract of Catharanthus roseus recorded maximum content of phenols compared to other extracts. Costus pictus root and leaf extracts exhibited a significant amount of flavonoids and tannins respectively. Further studies were focused on isolation and characterization of each plant compounds.

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Leaf histochemistry analysis of four medicinal species from Cerrado

Cited by 18 publications

References 33 publications

Flavonoides presentes nos extratos da campomanesia xanthocarpa Berg

Flavonoides presentes nos extratos da campomanesia xanthocarpa Berg

Use of a temporary immersion bioreactor system for the sustainable production of thapsigargin in shoot cultures of Thapsia garganica

Histochemical and Phytochemical Analysis of Medicinally Important Plants

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