Leafy Stems of Phagnalon saxatile subsp. saxatile from Algeriaas a Source of Chlorogenic Acids and Flavonoids with Antioxidant Activity: Characterization and Quantification Using UPLC-DAD-ESI-MSn

Abstract

Phagnalon saxatile subsp. saxatile is a wild species widespread in Algeria which is utilized for medicinal purposes as analgesic and anticholesterolemic. However, information is still scarce regarding its phytochemical content. The objective of this study was to identify and quantify the phenolic compounds from different extracts of its leafy stems. For this purpose, the effects of four extracting solvents were investigated on the content of phenolic compounds and the antioxidant activity of this plant. The extracts prepared with polar solvents (methanol and water) contained higher amounts of phenolic compounds and showed better antioxidant activity than the extracts with apolar solvents (hexane, dichloromethane). The methanolic extract, richest in total phenolic and total flavonoid, had significant antioxidant activity as regarded by DPPH° scavenging capacity (IC50 of 5.5 µg/mL), ABTS+° scavenging capacity (IC50 of 63.8 µg/mL) and inhibition of oxidation of linoleic acid (IC50 of 22.7 µg/mL), when compared to synthetic antioxidants. Chlorogenic acids and several flavonoids were identified and quantified by UPLC-DAD-MSn. The di-O-caffeoylquinic acids isomers were the most concentrated phenolics (25.4 mg/g DW) in the methanolic extract.

Identification of Ajuga Iva Extracts as Potential Candidates for Antidysmenorrhea Targeting Human COX2 and PGE2S‐1 through In Vitro and In Silico Drug Repurposing Approach

“…The extraction method involved the use of a Soxhlet apparatus, with methanol serving as a solvent, following the protocol described by Haddouchi et al [13] . Through this method, the solvent was completely evaporated, resulting in a methanol extract with an extraction yield up to 19.1 %.…”

“…The extraction method involved the use of a Soxhlet apparatus, with methanol serving as a solvent, following the protocol described by Haddouchi et al [13] Through this method, the solvent was completely evaporated, resulting in a methanol extract with an extraction yield up to 19.1 %. The specimen was meticulously gathered and preserved at 4 °C for subsequent examination and possible application in scientific research.…”

Assessment of Antioxidant and Antimutagenic Properties of Red and White Wine Extracts In Vitro

“…These compounds are mostly found in fruits, cereals, vegetables, and beverages [ 14 , 15 ]. Phenolic acids, flavonoids, stilbenes, and lignans are among the main polyphenolic categories that exert antioxidant activity by scavenging free radicals and disrupting oxidative reactions [ 14 , 16 ]. Studies with diets rich in these polyphenolic compounds have been found to shield from oxidative stress and protect from a wide range of diseases such as osteoporosis, cancer, diabetes, cardiovascular, and neurodegenerative diseases [ 6 , 17 , 18 , 19 , 20 , 21 ]; these studies add a translational value to the in vitro studies that have investigated the antioxidant potential of new compounds from natural products or foods.…”