Learning associated increase in heat shock cognate 70 mRNA and protein expression

Pizarro, José María Sánchez-Marín; Haro, Luis S.; Barea-Rodrı́guez, Edwin J.

doi:10.1016/s1074-7427(02)00008-4

Cited by 35 publications

(30 citation statements)

References 59 publications

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“…Animals were exposed to the same spatial cues, but without an escape platform, mice did not develop an association between the extra-maze cues and the location of the platform (Pizarro et al, 2003;Sunyer et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

Hippocampal synapsin isoform levels are linked to spatial memory enhancement by SGS742

John¹,

Sunyer²,

Höger³

et al. 2009

Hippocampus

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Synapsins are essential proteins for synaptic plasticity and there is no information available for their role in cognitive enhancement (CE) of spatial memory formation. It was therefore the aim of the study to link individual synapsin proteins and their isoforms to spatial memory formation enhanced by SGS742 in the mouse. Extracted hippocampal proteins from a cognitive study treating OF1 mice with the cognitive enhancer SGS742 and tested in the Morris water maze, were run on two-dimensional gel electrophoresis. Subsequently, protein spots were unambiguously identified by qQ-TOF mass spectrometry. Quantification of proteins from four groups (NaCl-treated mice, SGS742-treated mice, SGS742-treated yoked controls, and NaCl-treated yoked controls) was carried out according to an in-gel stable isotope labeling method. A total of 17 protein spots representing synapsin isoforms were identified and quantified. Using quantification of individual synapsin isoforms showed that these can be clearly assigned to CE by the GABAB antagonist SGS742. Quantitative determination of individual synapsin isoform showed an increase in SGS742-treated mice (mean+/-SD) of ratios between light and heavy stable isotope labeled synapsin protein (SGS742 vs. controls: 2.19+/-0.41 for synapsin Ia, and 1.41+/-0.81 for synapsin IIa). Synapsins Ib and IIb were not linked to CE. The NaCl-treated controls and the use of yoked controls that were ruling out swimming- and stress-mediated changes of synapsins, unequivocally allow to propose a role for synapsins Ia and IIa in the mechanism of CE of spatial memory formation.

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Section: Methodsmentioning

confidence: 99%

Hippocampal synapsin isoform levels are linked to spatial memory enhancement by SGS742

John¹,

Sunyer²,

Höger³

et al. 2009

Hippocampus

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“…The fact that 70-kDa HSPs are synthesized in the postsynaptic structure of most neuronal cells (Suzuki et al, 1999) suggests that their expression might also be a marker for increase in metabolic activity in brain areas engaged in cognitive processing (Pizzaro et al, 2003). In particular, hsp72 may be induced to amplify the constitutive function of hsc70, i.e., to chaperone the protein synthesis required for the storage of newly acquired information or the implementation of novel behavioral programs.…”

Section: Introductionmentioning

confidence: 99%

Hippocampal 72‐kDa heat shock protein expression varies according to mice learning performance independently from chronic exposure to stress

et al. 2005

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The possibility that the inducible 72-kDa heat shock protein (hsp72) is involved in learning-related plasticity mechanisms was investigated in two inbred strains of mice that show spontaneous differences in spatial learning performance as well as an opposite reactivity to stress. Induction of hsp72 after radial maze training was measured by immunoblotting in the hippocampus of C57BL/6 (C57) and DBA/2 (DBA) inbred mice exposed or nonexposed to chronic acoustic stress. In agreement with previous studies, inter-strain differences in radial maze performance were found in nonstressed mice with C57 mice showing the higher scores. Chronic acoustic stress, however, impaired performance in the high-learner C57 strain and improved performance in the low-learner DBA strain. Western blot analysis revealed that post-training expression of hsp72 was low in the condition each strain was showing the higher-performance (nonstressed C57 and stressed DBA) and high in the condition each strain was showing the lower performance (stressed C57 and nonstressed DBA). These findings indicate that expression of hsp72 in the hippocampus varies as a function of the learning performance independently from exposure to chronic acoustic stress.

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“…Chaperones have been reported to play a role in the mechanism of spatial memory formation (Pizarro et al, 2003;Zheng et al, 2009) and cognitive enhancement in the Morris water maze (Sunyer et al, 2008b). In the paradigm of spatial memory used herein, heat shock proteins 75 kDa and heat shock cognate 71 kDa protein were different between trained and yoked animals.…”

Section: Discussionmentioning

confidence: 83%

Proteins linked to spatial memory formation of CD1 mice in the multiple T‐maze

Patil

Heo

et al. 2011

Hippocampus

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In own previous work CD1 mice were tested in the Multiple T-maze (MTM), a robust land maze allowing determination of latency to reach the goal box with food reward and to evaluate correct decisions made on the way to the goal box. Herein, hippocampi of these animals were used for the current study with the aim to investigate differences in protein levels between trained and yoked mice and, moreover, to determine differences in protein levels between trained and yoked mice with and without memory formation in the MTM. Three training sessions were carried out for four training days each, followed by probe trials on Days 5 and 12. Good and no-performers in the MTM were separated based on means and median of latency to reach the goal box on probe trial Day 12. Six hours following the probe trial on Day 12, animals were sacrificed and hippocampi were taken. Proteins were extracted and run on two-dimensional gel electrophoresis, spots were quantified and differentially expressed proteins were identified by mass spectrometry using an ion trap. Levels of 17 proteins were significantly different in trained vs. yoked mice. Seven proteins were differentially expressed comparing trained vs. yoked mice from good and no-performers. A series of proteins were significantly correlated with latency and may link these proteins to spatial memory formation. Differential protein expression in trained vs. yoked mice and in good and no-performers may allow insight into spatial memory formation as well as represent tentative pharmacological targets.

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Learning associated increase in heat shock cognate 70 mRNA and protein expression

Cited by 35 publications

References 59 publications

Hippocampal synapsin isoform levels are linked to spatial memory enhancement by SGS742

Hippocampal synapsin isoform levels are linked to spatial memory enhancement by SGS742

Hippocampal 72‐kDa heat shock protein expression varies according to mice learning performance independently from chronic exposure to stress

Proteins linked to spatial memory formation of CD1 mice in the multiple T‐maze

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