Leaves metabolomic profiling of Musa acuminata accessions using UPLC–QTOF–MS/MS and their antioxidant activity

Sonibare, Mubo A.; Ayoola, Ibukun O.; Guèye, Badara; Abberton, Michael T.; Dsouza, Roy N.; Kuhnert, Nikolai

doi:10.1007/s11694-018-9725-4

Cited by 19 publications

(15 citation statements)

References 33 publications

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“…In the same manner, peak 15 showed the characteristic fragmentation pattern of kaempferol-O-glucuronide [33]. The isomer compounds at peaks 13 and 14 showed deprotonated ions at m/z 447 but different daughter ions at m/z 285 and 301, characteristic of kaempferol-O-glucoside [34] and quercetin-O-rhamnose [35], respectively. Peak 16, with a deprotonated ion at m/z 563, showed a daughter ion at m/z 285 (loss of 278 Dalton, characteristic of rhamnosyl pentoside) and thus was identified as kaempferol-3-O-rhamnosylpentose.…”

Section: Phenolic Compounds Identification In Persicaria Maculosa Extmentioning

confidence: 75%

Phenolic Fingerprinting, Antioxidant, and Deterrent Potentials of Persicaria maculosa Extracts

Quesada-Romero

Fernández-Galleguillos

Bergmann

et al. 2020

Molecules

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Persicaria maculosa (Polygonaceae) (known as lady’s thumb) is an annual morphologically variable weed that is widely distributed in Chile. The purpose of this study was to investigate the antifeedant potential of methanolic (MeOH), ethanolic (EtOH), and dichloromethane (DCM) extracts from the aerial parts of this plant collected in the Valparaíso and Curicó provinces (Chile) and relate this activity to the antioxidant capacity and the presence of phenolic compounds in the extracts. A phenolic profile based on HPLC-ESI-MS/MS allowed the identification of 26 phenolic compounds, most of them glycosyl derivatives of isorhamnetin, quercetin, and kaempferol. In addition, the total phenolic content (TP), total flavonoids (TF), and antioxidant activity measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion scavenging (O2−), ferric-reducing antioxidant power (FRAP), and cupric-reducing antioxidant capacity (CUPRAC) of the extracts are reported. The antifeedant potentials of the plant extracts were tested against Epilachna paenulata, Pseudaletia adultera, Macrosiphum euphorbiae, and Diaphorina citri insects for the first time. The activity against the aphid M. euphorbiae was significant for the DCM extracts of plants from Valparaíso and Curicó (settling % = 23% ± 4% and 23% ± 5%, respectively). The antifeedant activities against the beetle E. paenulata and the lepidoptera P. adultera were significant for Valparaíso extracts, especially when tested against E. Paenulata (IFP = 1.0 ± 0.0). Finally, the MeOH and EtOH extracts from Valparaíso plants reduced the diet consumption of the psilid D. citri (p < 0.05). The results showed that P. maculosa is a good source of flavonoids with some antioxidant capacities and has potential interest as botanical eco-friendly alternative with deterrent activity.

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Section: Phenolic Compounds Identification In Persicaria Maculosa Extmentioning

confidence: 75%

Phenolic Fingerprinting, Antioxidant, and Deterrent Potentials of Persicaria maculosa Extracts

Quesada-Romero

Fernández-Galleguillos

Bergmann

et al. 2020

Molecules

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“…7-Methoxyquercetin-O-hexose isomers are polyphenolic compound detected in Ajwa and widely reported as potential anti-oxidant and antimicrobial agent. [33,34] Caffeic acid was detected also in both Ajwa and Al-Qasim extracts. Caffeic acid is an organic compound and a potent antioxidant classified as a hydroxycinnamic acid.…”

Section: Resultsmentioning

confidence: 93%

Phenolic profiling and antibacterial potential of Saudi Arabian native date palm (Phoenix dactylifera) cultivars

Mediani

2020

International Journal of Food Properties

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Date Palm (Phoenix dactylifera) is one of the important fruit enriched with an impressive list of essential nutrients and a wide range of phenolic compounds with high antibacterial compounds. In order to provide further detailed information on phenolic compounds and antibacterial activities of Date Palm (Phoenix dactylifera), the current study was devoted to investigate and compare three different highly consumable varieties of date palm (Phoenix dactylifera) cultivars, namely Ajwa, Khalas Alkharj, and Al-Qasim for their broad spectrum antibacterial effect and phenolic compounds. The antibacterial properties of their methanolic extracts were evaluated against Streptococcus pnuemoniae, Bacillus subtilis, Klebsiella pneumonia, Staphylococcus aureus, Escherichia coli. All of the three cultivars displayed a broad spectrum of inhibitory effect against these pathogenic bacteria. Moverover, the LC-Linear Ion Quadrupole Mass Spectrometric Analysis (LC-MS and LC-MS/MS Tandem Mass Spectrometry) analysis revealed the presence of 3,30-di-O-methyl ellagic acid, 7-Methoxyquercetin-O-hexose isomers, caffeic acid, ferulic acid, isomers of quercentin-rutinoside, kaempferol methylether, p hydroxybenzoic acid, phytol, punicalagin and quercetin-3-O-glucoside (isoquercitrin) were in Ajwa, 3,30-di-O-methyl ellagic acid isomers, 3,30-di-O-methyl ellagic acid, caffeic acid derivative, cafeic acid, caffeoyl glucose, caffeoylshikimic acid, ferulic acid, guaiacyl(8-5)ferulic acid hexoside isomer, isorhamnetin-3-Ohexoside, oxooctadecanoic acid, punicalagin and quercetin derivativein Al-Qasim extracts while 2(3,4-Dihydroxyphenyl)-7-hydroxy-5-benzene propanoic acid isomer, 2 (3,4-Dihydroxyphenyl)-7-hydroxy-5-benzene propanoic acid, decanoic acid derivative, diosmetin, kaempferol-3-rutinoside, linolenic acid, oxooctadecanoic acid conjugate and punicalagin in the extract of Khalas date. The findings of the current study may increase our knowledge about the importance of dates in diet and can be utilized as natural antimicrobial agents in the foods industry. ARTICLE HISTORY

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“…For a 5 g dry leaf sample, both methods consumed 100 mL of 70% MeOH. The Soxhlet extraction took 9 h and gave 20–60% recoveries, while the sonication took 30 min and gave 18–23% recoveries [ 7 ]. As a comparison, our optimized sPLE method required smaller sample size (0.1 g), consumed less hazardous solvent (20 mL of 50% MeOH), and achieved a significant higher extraction efficiency in less time (10 min, 63–107% recoveries).…”

Section: Resultsmentioning

confidence: 99%

“…Sample preparation is the first step in plant metabolomics studies where the extraction process is a determining factor for data quality. Conventional extraction techniques such as Soxhlet extraction suffer from long extraction time and high solvent-consumption [ 7 ]. Alternative strategies such as ultrasound-assisted extraction (UAE) are advantageous in terms of simplicity but oftentimes have low recovery and require an additional cleanup step [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Selective pressurized liquid extraction of plant secondary metabolites: Convallaria majalis L. as a case

Liang

Nielsen

Christensen

2020

Analytica Chimica Acta: X

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A fast and efficient selective pressurized liquid extraction (sPLE) method was developed to extract secondary metabolites from complex plant matrix. Convallaria majalis L., a plant producing toxic steroids, was used as proof-of-concept. The method was optimized in the aspects of preheating, dispersant, extraction temperature and solvent, and the use of C18 as in-cell cleanup sorbent. Eight authentic natural steroids with diverse sugar moieties and hydrophobicities were selected as reference analytes and spiked to 0.1 g dried leaves. The extraction performance was evaluated based on the analytes’ stability, recovery, matrix effect in the electrospray interface and the level of co-extractives. With the optimal method, the extraction was finished in 10 min. A colorless extract was obtained with recoveries ranging from 63% to 107% and absolute matrix effects ranging from 3% to 27%. The optimized method was validated by extracting 0.1 g, 0.2 g and 0.4 g spiked plant samples; method accuracy and precision were assessed by recoveries and relative standard deviations of the combined extraction-analysis workflow. The method was also tested on soil samples and indicated its suitability for measuring secondary metabolites in multiple environmental matrices. To our knowledge, this is the first time sPLE has been reported to extract plant secondary metabolites from a complex plant matrix, with satisfactory recoveries and low matrix effects. This is also the first time (s)PLE has been reported to extract plant secondary metabolites from soil. We envision the method be coupled with liquid chromatography-electrospray ionization-high resolution mass spectrometry in a standard metabolomics workflow to facilitate plant metabolomics studies.

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Leaves metabolomic profiling of Musa acuminata accessions using UPLC–QTOF–MS/MS and their antioxidant activity

Cited by 19 publications

References 33 publications

Phenolic Fingerprinting, Antioxidant, and Deterrent Potentials of Persicaria maculosa Extracts

Phenolic Fingerprinting, Antioxidant, and Deterrent Potentials of Persicaria maculosa Extracts

Phenolic profiling and antibacterial potential of Saudi Arabian native date palm (Phoenix dactylifera) cultivars

Selective pressurized liquid extraction of plant secondary metabolites: Convallaria majalis L. as a case

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