Lectin from Crataeva tapia bark exerts antitumor, anti-inflammtory and analgesic activities

Araújo, Regina Maria Sousa de; Vaz, Antônio Fernando de Melo; Aguiar, Jaciana dos Santos; Coelho, Luana Cassandra Breitenbach Barroso; Paiva, Patrícia Maria Guedes; Melo, Ana Maria Mendonça de Albuquerque; Silva, Teresinha G.; Correia, Maria T.S.

doi:10.1007/s13659-011-0014-8

Cited by 18 publications

(13 citation statements)

References 18 publications

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“…A number of its biological properties have been characterized, including delay in clot formation by impairing the intrinsic pathway of coagulation cascade [18]. In addition, CrataBL was shown to exhibit anti-inflammatory, analgesic [19], insecticidal [17], and anti-tumor [19] activities.…”

Section: Introductionmentioning

confidence: 99%

Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines

et al. 2013

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A protein isolated from the bark of Crataeva tapia (CrataBL) is both a Kunitz-type plant protease inhibitor and a lectin. We have determined the amino acid sequence and three-dimensional structure of CrataBL, as well as characterized its selected biochemical and biological properties. We found two different isoforms of CrataBL isolated from the original source, differing in positions 31 (Pro/Leu); 92 (Ser/Leu); 93 (Ile/Thr); 95 (Arg/Gly) and 97 (Leu/Ser). CrataBL showed relatively weak inhibitory activity against trypsin (Kiapp = 43 µM) and was more potent against Factor Xa (Kiapp = 8.6 µM), but was not active against a number of other proteases. We have confirmed that CrataBL contains two glycosylation sites and forms a dimer at high concentration. The high-resolution crystal structures of two different crystal forms of isoform II verified the β-trefoil fold of CrataBL and have shown the presence of dimers consisting of two almost identical molecules making extensive contacts (∼645 Å2). The structure differs from those of the most closely related proteins by the lack of the N-terminal β-hairpin. In experiments aimed at investigating the biological properties of CrataBL, we have shown that addition of 40 µM of the protein for 48 h caused maximum growth inhibition in MTT assay (47% of DU145 cells and 43% of PC3 cells). The apoptosis of DU145 and PC3 cell lines was confirmed by flow cytometry using Annexin V/FITC and propidium iodide staining. Treatment with CrataBL resulted in the release of mitochondrial cytochrome c and in the activation of caspase-3 in DU145 and PC3 cells.

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Section: Introductionmentioning

confidence: 99%

Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines

et al. 2013

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“…Lectins are carbohydrate binding proteins, of nonimmunogenic origin, that bind specifically and reversibly to different types of carbohydrates or glycoproteins and can be obtained from several sources, mainly from vegetal [ 3 ]. Several plant lectins have been demonstrated to possess a variety of biological activities including antitumor [ 4 – 6 ], anti-inflammatory [ 7 , 8 ], antimicrobial [ 9 – 11 ], analgesic [ 4 ], antioxidant [ 3 ] insecticidal [ 2 , 12 – 14 ], anticoagulant [ 15 ], and hypoglycemic [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Lectin fromCrataeva tapiaBark Improves Tissue Damages and Plasma Hyperglycemia in Alloxan-Induced Diabetic Mice

Rocha

Araújo²,

Fonseca³

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Crataeva tapia is a plant popularly used for diabetes treatment, in Brazil. Progressive decline in renal and hepatic functions has been described in patients with diabetes mellitus, and mortality rate is increased in patients with chronic liver and renal disease. This study aimed to evaluate whether Crataeva tapia bark lectin (CrataBL) improves hyperglycemia and renal and hepatic damage in diabetic mice. CrataBL was purified by ion exchange chromatography on CM-cellulose, and intraperitoneal administration of CrataBL to alloxan-induced diabetic mice at dose of 10 mg/Kg/day and 20 mg/Kg/day for 10 days significantly reduced serum glucose levels by 14.9% and 55.9%, respectively. Serum urea, creatinine, aspartate aminotransferase, and alanine aminotransferase were also significantly reduced after treatment with both doses of CrataBL. Furthermore, histological analysis of liver, kidney, and pancreas revealed an improvement in the tissue morphology upon treatment with CrataBL. The results suggest that CrataBL has a beneficial hypoglycemic activity and improves the renal and hepatic complications of diabetes. Therefore, this lectin may be a promising agent for the treatment of diabetes, and this might be the basis for its use in the folk medicine as an alternative treatment to manage diabetes-related complications such as hyperglycemia and tissue damage.

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“…Lectin was isolated from Crataeva tapia bark that exhibited antiinflammatory and antinociceptive potential along with its significant anti-tumour property. 26…”

Section: Bacopa Monnieri Linn (Plantaginaceae)mentioning

confidence: 99%

Plants and Plant Constituents with Analgesic and Anti-inflammatory Activities: A Systematic Review

Lisa

Islam

Qais

2020

Dhaka Univ. J. Pharm. Sci

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Medicinal plants with potential therapeutic activities are a tremendous resources of prospective drug candidates. NSAIDs, opiates, and other anti-inflammatory & analgesic agents exhibit several unwanted side-effects. Thus, the development of new active compounds with minimum adverse effects necessitates an emergence. This study aims to provide a comprehensive summary of plant species and reported phytoconstituents with analgesic and inti-inflammatory activities. Eighty-seven species from fifty-two plant families with reported constituents and activities have been included in this review. In-depth research in the area of screening novel analgesic and antiinflammatory agents from natural sources followed by the investigation of their pharmacological properties and clinical applications may lead to the generation of new active agents with better therapeutic activity and selectivity in the future. Dhaka Univ. J. Pharm. Sci. 19(2): 207-224, 2020 (December)

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Lectin from Crataeva tapia bark exerts antitumor, anti-inflammtory and analgesic activities

Cited by 18 publications

References 18 publications

Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines

Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines

Lectin fromCrataeva tapiaBark Improves Tissue Damages and Plasma Hyperglycemia in Alloxan-Induced Diabetic Mice

Plants and Plant Constituents with Analgesic and Anti-inflammatory Activities: A Systematic Review

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