Lectin histochemistry of microvascular endothelium in chick and quail musculature

Naňka, Ondřej; Peumans, Willy J.; Damme, Els J.M. Van; Pfüller, Uwe; Valášek, Petr; Halata, Zdeněk; Schumacher, Udo; Grim, Miloš

doi:10.1007/s004290100212

Cited by 13 publications

(21 citation statements)

References 22 publications

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“…Three days after injection, the host vessels were visualized in embryo tissue sections by using sambucus nigra agglutinin (SNA). Figure 6A shows that the Nrp1 ϩ / Flk1 ϩ -injected cells integrated within the new forming vessels, as indicated by the alignment of the YFP signal with the signal given by the chicken endothelial-specific SNA (43). Identical results were obtained by using the W4 ES cell line (Supplemental Fig.…”

Section: Neuropilin-1 and Flk1 Identify A Discrete Nonclonal Populatmentioning

confidence: 76%

Microenvironment drives the endothelial or neural fate of differentiating embryonic stem cells coexpressing neuropilin‐1 and Flk‐1

et al. 2008

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The observation that the architecture of the cardiovascular and nervous systems is drawn by common guidance cues and the closeness between neural progenitors and endothelial cells in the vascular niche strongly suggests the existence of links between endothelial and neural cell fates. We identified an embryonic stem cell-derived discrete, nonclonal cell population expressing the two vascular endothelial growth factor receptors neuropilin-1 (Nrp1) and Flk1 that differentiates in vitro toward endothelial or neural phenotypes depending on microenvironmental cues. When microinjected in the chick embryo, Nrp1(+) cells integrate within the host, developing vessels and brain, and acquire endothelial and neural markers, respectively. These results show that precursors of endothelial cells and precursors of neural cells arise from the same pool of differentiating embryonic stem cells and share the expression of Nrp1 and Flk1. These data reinforce the parallelism between vascular and nervous system at the level of cell fate and commitment and open new perspective in regenerative medicine of neurovascular diseases.

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Section: Neuropilin-1 and Flk1 Identify A Discrete Nonclonal Populatmentioning

confidence: 76%

Microenvironment drives the endothelial or neural fate of differentiating embryonic stem cells coexpressing neuropilin‐1 and Flk‐1

et al. 2008

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“…Lectin histochemistry has been frequently used to visualize the morphology of vascular networks in a variety of organs, either on tissue sections (Porter et al, 1990;Hansen-Smith et al, 1988;Nanka et al, 2001;Beltrão et al, 2003;Jilani et al, 2003;Cebasek et al, 2004) or using intravascular perfusion (Thurston et al, 1998Murphy et al, 1999;Debbage et al, 2001;Jilani et al, 2003). However, lectins have been only rarely used to delineate the vascular tree in the central nervous system (Porter et al, 1990), although their affinity to brain endothelium has been extensively characterized (Nag 1985a, b;Vorbrodt, 1986;Vorbrodt et al, 1986;Alroy et al, 1987;Fatehi et al, 1987;Porter et al, 1990;Debbage et al, 1996;Gerhart et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Lycopersicon esculentum lectin: an effective and versatile endothelial marker of normal and tumoral blood vessels in the central nervous system

Mazzetti¹,

Frigerio²,

Gelati³

et al. 2009

Eur J Histochem

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The binding of Lycopersicon esculentum lectin (LEA) to the vascular endothelium was studied in the central nervous system of rat, mouse and guinea pig at different developmental ages, and in a gliosarcoma model. Our observations showed that LEA consistently stained the entire vascular tree in the spinal cord and in the brain of all animal species at all developmental ages investigated. In the tumor model, the staining of the vascular network was very reproducible, enabled an easy identification of vascular profiles and displayed a higher efficiency when compared to two other commonly used vascular marker (EHS laminin and PECAM-1). Moreover, our results showed that LEA staining was comparable in both vibratome and paraffin sections and could be easily combined with other markers in double labeling experiments. These observations indicate that LEA staining may represent an effective and versatile endothelial marker for the study of the vasculature of the central nervous system in different animal species and experimental conditions.

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“…Next 7-m thick sections were cut, as previously described (13). Endothelial cells were stained using biotin-labeled Sambucus nigra lectin (1/3,000; Vector, Burlingame, CA) (14). For the study of apoptosis, CAMs were fixed overnight in 4% paraformaldehyde at 4°C, dehydrated in graded alcohol, cleared in xylene, and embedded in paraffin.…”

Section: Methodsmentioning

confidence: 99%

Hyperglycemia-Induced Defects in Angiogenesis in the Chicken Chorioallantoic Membrane Model

et al. 2004

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Diabetes is associated with abnormal angiogenesis. Increased angiogenesis contributes to severe forms of diabetic retinopathy, but angiogenesis is decreased in response to myocardial ischemia in diabetic patients. We evaluated the direct effect of hyperglycemia on angiogenesis in the chicken chorioallantoic membrane assay, a model of active neoangiogenesis. Hyperglycemia, lasting up to 7 days, was induced in 7-day-old chick embryos by a single intravitellus glucose injection. Control embryos received either water (volumic control) or mannitol (osmotic control). Hyperglycemia decreased angiogenesis in this model from the 5th day on. The pattern and expression level of the main vascular growth factors' genes were not altered by hyperglycemia, as assessed by in situ hybridization and semiquantitative RT-PCR. As early as 2 days after hyperglycemia was induced, an increased apoptosis of endothelial cells and pericytes was detected by transferase-mediated deoxyuridine triphosphate nick-end labeling assay and electron microscopy. In the meantime, endothelial cell proliferation was decreased, as assessed by incorporation of bromo-deoxyuridine. Hyperglycemia can therefore impair angiogenesis without altering the expression level of vascular growth factors through induction of apoptosis and decreased proliferation of endothelial cells. Diabetes 53:752-761, 2004

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Lectin histochemistry of microvascular endothelium in chick and quail musculature

Cited by 13 publications

References 22 publications

Microenvironment drives the endothelial or neural fate of differentiating embryonic stem cells coexpressing neuropilin‐1 and Flk‐1

Microenvironment drives the endothelial or neural fate of differentiating embryonic stem cells coexpressing neuropilin‐1 and Flk‐1

Lycopersicon esculentum lectin: an effective and versatile endothelial marker of normal and tumoral blood vessels in the central nervous system

Hyperglycemia-Induced Defects in Angiogenesis in the Chicken Chorioallantoic Membrane Model

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