Lectin probes of glycoconjugates in human salivary glands: 1

The purpose of the present investigation was to study the histogenesis of the mucoepidermoid carcinoma of the salivary glands. Eleven cases of mucoepidermoid carcinoma of the minor salivary glands and five of the major glands were extensively studied employing immunohistochemical and fluorescent microscopic techniques. Both the intermediate cells and the duct cells showed a rather similar pattern of reactivity for vimentin, actin and EMA. Also, the intermediate cells and the myoepithelial cells showed a similar reaction pattern for keratin and UGA-1. The intermediate, myoepithelial and duct cells shared a similar reaction pattern for desmin, myosin, CEA, and S-100 protein. However, the rest of the tumor markers studied (AFP, PNA and WGA) were found to be non contributary. We also found that the intermediate and to some extent the epidermoid tumor cells showed a positive reaction with Azophloxine GA, which is a selective stain for myoepithelial cells in the normal glands. Based on these findings, the duct cells, the myoepithelial cell in the normal glands and the intermediate cells of the mucoepidermoid carcinoma share certain similar characteristics. The intermediate cells may actually be a mixed population, some having characteristics of the myopithelial cells and others of duct cells. These findings are relevant to the possible role of the intermediate cell in the histogenesis of the mucoepidermoid carcinoma.

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“…Our results on WGA binding to the normal salivary glands are consistent with some previous reports (25)(26)(27)(28). While MuRASE et at.…”

Section: Discussionsupporting

confidence: 93%

“…Therefore, it may serve as a marker for the degree of tumor differentiation. PNA binding to the normal glands in the present investigation showed a pattern similar to that in earlier reports (25)(26)(27). All cell types showed variable degrees of reactivity.…”

Section: Discussionsupporting

confidence: 91%

Immunohistochemical and fluorescent microscopic study of histogenesis of salivary mucoepidermoid carcinoma

Hassanin

Ghosh

Das

et al. 1989

J Oral Pathology Medicine

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“…The lectins UEA I, SBA, DBA, MPA and WGA described in the intraglandular duct epithelium also revealed binding sites in the parotid duct. According to studies of Tolson et al (1985), ConA and WGA occurred in serous acinar cells but not intraglandularly in the epithelium of small excretory ducts. In contrast to this, our results demonstrate that WGA-positive cells are also present in the epithelium of the main parotid duct.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial defence mechanisms of the human parotid duct

et al. 2006

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The parotid duct transports saliva from the gland into the oral cavity. However, its immune response properties, along with the secretion and moistening principles of the duct, have not yet been fully investigated. These properties may play an important role in protecting the parotid gland from infection and also prevent development of sialodocholithiasis, as the parotid duct -in contrast to the submandibular salivary duct -is often free of duct concrements. Up to now, only the parotid gland has been investigated, without regard to its duct. The present study analyses the structures of the parotid duct in their relations to antimicrobial defence mechanisms and rheological properties. Investigations were performed on 23 parotid ducts using histological, histochemical and immunohistochemical methods. Epithelial and goblet cells of the parotid duct synthesize a complex mucous layer that covers the epithelium. The viscosity is influenced by secreted mucins and TFF peptides. This layer contains carbohydrates including N -acetyl-glucosamine, N -acetyl-galactosamine, galactose, mannose, fucose and sialic acids. The lamina propria contains granulocytes, T lymphocytes and macrophages. IgA, produced by plasma cells in the subepithelial layer, is frequently integrated in the secretory product. Synthesized mucins, TFF peptides, carbohydrates and immunoglobulins form a complex layer that can be expected to prohibit infection and enables salivary flow. Our study demonstrates that the steady secretion of the parotid gland, together with the ductal cellular and biochemical immune protection system, is likely to thwart ascending infections in the parotid duct and gland.

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“…Lectin histochemistry can also reveal subtle alterations in glycosylation between otherwise indistinguishable cells (RHODES and MILTON, 1998;ROTH, 2011). Some studies have been carried out to determine the glycoconjugates found in parotid glands of some species by lectin histochemistry SPICER, 1983, 1984;LADEN;SCHULTE and SPICER, 1984;TOLSON, DALEY and WYSOCKI, 1985;JEZERNIK and PIPAN, 1986;VIGNESWARAN, HANEKE and HORNSTEIN, 1989;ACCILI, MENGHI, BONDI et al, 1992;GARGIULO, PEDINI and CECCARELLI, 1993;GARGIULO, 1994, 1995;PEDINI, CECCARELLI, GARGIULO et al, 1997;KIMURA, HABATA, ENDO et al, 1998;SÖZMEN, BROWN and EVESON, 1999;ADNYANE, WRESDIYATI, WIBAWAN et al, 2006;MUNYALA, LIUMSIRICHAROEN, PONGKET et al, 2009;SOBRAL, REGO, CAVALACANTI et al, 2010). To the best of our knowledge, there is no study that determines the distribution of glycoconjugates in sheep (Ovis aries) parotid glands by use of lectins.…”

Section: Introductionmentioning

confidence: 99%

Glycoconjugate composition of ovine parotid glands elucidated by lectins

2016

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Introduction: This study aimed to determine the distribution of glycoconjugates found in sheep (Ovis aries) parotid glands by lectin histochemistry. Methods: Following routine histological tissue processing, tissue sections were labelled with the lectins Con-A (Canavalia ensiformis), UEA-I (Ulex europaeus), BSA-I-B 4 (Bandeiraea simplicifolia), PNA (Arachis hypogaea), WGA (Triticum vulgaris) and SBA (Glycine max). Results: The results of lectin staining indicated that fucose sugar was the most abundant sugar on the surface of serous cells, although absence of N-acetylgalactosamine on the serous cells surface. Fucose, N-acetylglucosamine, mannose, galactose, and N-acetylgalactosamine were present on the surface of duct cells. All lectins stained with duct epithelial cells in a similar manner -from weak to moderate. Serous cells were labelled with all the lectins, except for PNA, in various degrees. Conclusion: The data obtained from this study can provide new insight into characterizing the glycoconjugate profiles in different species in an effort to be capable of understanding detailed structure and function of parotid gland in both normal-and abnormal states.

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Lectin probes of glycoconjugates in human salivary glands: 1

Cited by 20 publications

References 15 publications

Immunohistochemical and fluorescent microscopic study of histogenesis of salivary mucoepidermoid carcinoma

Immunohistochemical and fluorescent microscopic study of histogenesis of salivary mucoepidermoid carcinoma

Antimicrobial defence mechanisms of the human parotid duct

Glycoconjugate composition of ovine parotid glands elucidated by lectins

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