Lectins from Synadenium carinatum (ScLL) and Artocarpus heterophyllus (ArtinM) Are Able to Induce Beneficial Immunomodulatory Effects in a Murine Model for Treatment of Toxoplasma gondii Infection

Souza, Leandro Peixoto Ferreira de; Ramos, Eliézer Lucas Pires; Santana, Silas Silva; Silva, Murilo Vieira da; Santiago, Fernanda Maria; Mineo, Tiago Wilson Patriarca

doi:10.3389/fcimb.2016.00164

Cited by 13 publications

(6 citation statements)

References 71 publications

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“…Furthermore, the life span of ArtinM-stimulated neutrophils decreased [42]. Additionally, in a murine model of Toxoplasma gondii infection ArtinM induced the production of anti-inflammatory cytokines and increased NO levels as well as reducing the parasitic load [43].…”

Section: Discussionmentioning

confidence: 94%

The lectin ArtinM activates RBL-2H3 mast cells without inducing degranulation

et al. 2020

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Mast cells are connective tissue resident cells with morphological and functional characteristics that contribute to their role in allergic and inflammatory processes, host defense and maintenance of tissue homeostasis. Mast cell activation results in the release of pro-inflammatory mediators which are largely responsible for the physiological functions of mast cells. The lectin ArtinM, extracted from Artocarpus heterophyllus (jackfruit), binds to D-manose, thus inducing degranulation of mast cells. ArtinM has several immunomodulatory properties including acceleration of wound healing, and induction of cytokine release. The aim of the present study was to investigate the role of ArtinM in the activation and proliferation of mast cells. The rat mast cell line RBL-2H3 was used throughout this study. At a low concentration (0.25μg/mL), ArtinM induced mast cell activation and the release of IL-6 without stimulating the release of pre-formed or newly formed mediators. Additionally, when the cells were activated by ArtinM protein tyrosine phosphorylation was stimulated. The low concentration of ArtinM also activated the transcription factor NFkB, but not NFAT. ArtinM also affected the cell cycle and stimulated cell proliferation. Therefore, ArtinM may have therapeutic applications by modulating immune responses due to its ability to activate mast cells and promote the release of newly synthesized mediators. Additionally, ArtinM could have beneficial effects at low concentrations without degranulating mast cells and inducing allergic reactions.

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Section: Discussionmentioning

confidence: 94%

The lectin ArtinM activates RBL-2H3 mast cells without inducing degranulation

et al. 2020

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“…Although a few antiviral drugs were approved by the FDA for COVID-19 treatment, the search for compounds with anti-SARS-CoV-2 activity is still a main global purpose. In this context, the lectins isolated from plants are proteins with broad applicability in biotechnology [ 78 ], and they can be used against different infections, such as protozoa, bacteria, and viruses [ 79 , 80 , 81 ]. These molecules act as carbohydrate-binding agents and are able to bind to glycans on the surface of SARS-CoV-2, altering the viral glycoprotein spike 3D conformation and tricking the interaction with the ACE2 cell receptor [ 82 ].…”

Section: Discussionmentioning

confidence: 99%

Mannose-Binding Lectins as Potent Antivirals against SARS-CoV-2

Grosche,

Souza,

Ferreira

et al. 2023

Viruses

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The SARS-CoV-2 entry into host cells is mainly mediated by the interactions between the viral spike protein (S) and the ACE-2 cell receptor, which are highly glycosylated. Therefore, carbohydrate binding agents may represent potential candidates to abrogate virus infection. Here, we evaluated the in vitro anti-SARS-CoV-2 activity of two mannose-binding lectins isolated from the Brazilian plants Canavalia brasiliensis and Dioclea violacea (ConBR and DVL). These lectins inhibited SARS-CoV-2 Wuhan-Hu-1 strain and variants Gamma and Omicron infections, with selectivity indexes (SI) of 7, 1.7, and 6.5, respectively for ConBR; and 25, 16.8, and 22.3, for DVL. ConBR and DVL inhibited over 95% of the early stages of the viral infection, with strong virucidal effect, and also protected cells from infection and presented post-entry inhibition. The presence of mannose resulted in the complete lack of anti-SARS-CoV-2 activity by ConBR and DVL, recovering virus titers. ATR-FTIR, molecular docking, and dynamic simulation between SARS-CoV-2 S and either lectins indicated molecular interactions with predicted binding energies of −85.4 and −72.0 Kcal/Mol, respectively. Our findings show that ConBR and DVL lectins possess strong activities against SARS-CoV-2, potentially by interacting with glycans and blocking virus entry into cells, representing potential candidates for the development of novel antiviral drugs.

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“…Bone marrow cells were isolated as described previously (Weischenfeldt and Porse, 2008 ; de Souza et al, 2016 ). Femurs and tibias from mice were removed, disinfected by immersion in 70% ethanol and the ends of each bone were cut.…”

Section: Methodsmentioning

confidence: 99%

Treatment with pCramoll Alone and in Combination with Fluconazole Provides Therapeutic Benefits in C. gattii Infected Mice

Jandú

Costa

Santos

et al. 2017

Front. Cell. Infect. Microbiol.

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Cryptococcus gattii is one of the main causative agents of cryptococcosis in immunocompetent individuals. Treatment of the infection is based on the use of antimycotics, however, the toxicity of these drugs and the increase of drug-resistant strains have driven the search for more effective and less toxic therapies for cryptococcosis. pCramoll are isolectins purified from seeds of Cratylia mollis, a native forage plant from Brazil, which has become a versatile tool for biomedical application. We evaluated the effect of pCramoll alone and in combination with fluconazole for the treatment of mice infected with C. gatti. pCramoll alone or in combination with fluconazole increased the survival, reduced the morbidity and improved mice behavior i.e., neuropsychiatric state, motor behavior, autonomic function, muscle tone and strength and reflex/sensory function. These results were associated with (i) decreased pulmonary and cerebral fungal burden and (ii) increased inflammatory infiltrate and modulatory of IFNγ, IL-6, IL-10, and IL-17A cytokines in mice treated with pCramoll. Indeed, bone marrow-derived macrophages pulsed with pCramoll had increased ability to engulf C. gattii, with an enhanced production of reactive oxygen species and decrease of intracellular fungal proliferation. These findings point toward the use of pCramoll in combination with fluconazole as a viable, alternative therapy for cryptococcosis management.

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Lectins from Synadenium carinatum (ScLL) and Artocarpus heterophyllus (ArtinM) Are Able to Induce Beneficial Immunomodulatory Effects in a Murine Model for Treatment of Toxoplasma gondii Infection

Cited by 13 publications

References 71 publications

The lectin ArtinM activates RBL-2H3 mast cells without inducing degranulation

The lectin ArtinM activates RBL-2H3 mast cells without inducing degranulation

Mannose-Binding Lectins as Potent Antivirals against SARS-CoV-2

Treatment with pCramoll Alone and in Combination with Fluconazole Provides Therapeutic Benefits in C. gattii Infected Mice

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