Leishmania chagasi: lipophosphoglycan characterization and binding to the midgut of the sand fly vector Lutzomyia longipalpis

Development of Leishmania (Leishmania) infantum chagasi in Its Natural Sandfly Vector Lutzomyia longipalpis

“…In the current study, we used L. infantum chagasi World Health Organization reference strain MHOM/ BR/1970/BH46. Promastigotes was cultured in medium 199 supplemented with 10% fetal bovine serum and other components 16 at 26 C. Axenic cultures of in vitro amastigotes were initiated from stationary-phase promastigotes, which were placed in a concentration of 5…”

“…Lipophosphoglycan, the major Leishmania surface glycoconjugate, protects the parasites from the enzyme activities of its host and mediates parasite attachment to the midgut of the sandfly. [8][9][10][11][12][13][14][15][16][17][18] After digestion, successful infection in a sandfly vector results in development of several promastigotes forms types named, according to their morphology, as procyclic, haptomonad, nectomonad, paramastigote, and metacyclic forms. 19 Only metacyclic forms transmitted through sandfly bites are able to begin an infection in vertebrate hosts.…”

Capacidade vetorial de Lutzomyia (Lutzomyia) cruzi (Diptera: Psychodidae) para Leishmania (Leishmania) infantum

“…Acredita-se que este escape seja mediado pela ação da enzima quitinase secretada pelo próprio parasita (SCHLEIN, et al, 1991, SHAKARIAN e DWYER, 2000 e também pelo inseto (RAMALHO-ORTIGAO et al, 2005). Já adesão ao epitélio é desempenhada principalmente pelo LPG, entre outros fatores (PIMENTA et al, 1992;SOARES et al, 2002).…”

Leishmania chagasi Cunha & Chagas, 1937: indigenous or introduced? A brief review

“…15,16,14 In contradiction to these authors who asserted that L. , which was well adapted to its phlebotomine sandfly vector, Phlebotomus dubosqi, in endemic countries in Europe, would have needed to promptly adapt to another species of phlebotomine sandfly vector, Lutzomyia longipalpis, from a continent that presents completely different weather and ecology from those in Europe. Additionally, it is also important to emphasize recent evidence suggesting that this speciation process between the parasite and its phlebotomine sandfly vector is strongly influenced by a specific interaction between the ligand glycoconjugate molecules, principally lipophosphoglycan (LPG), present on the plasmatic membrane surface of the metacyclic promastigote forms of Leishmania species and their receptors on the epithelial cell membrane of the midgut wall 2,22,21 of the phlebotomine sandfly vector . LPG has been implicated as a specific adhesion molecule that mediates the interaction of Leishmania with the midgut epithelium of the phlebotomine sandfly vector.…”