Leishmanicidal activity of the Agaricus blazei Murill in different Leishmania species

Valadares, Diogo G.; Duarte, Mariana C.; Oliveira, Jamil S.; Chávez-Fumagalli, Miguel Á.; Martins, Vívian T.; Costa, Lourena E.; Leite, João Paulo Viana; Santoro, Marcelo M.; Régis, Wiliam César Bento; Tavares, Carlos Alberto Pereira; Coelho, Eduardo Antônio Ferraz

doi:10.1016/j.parint.2011.06.001

Cited by 76 publications

(60 citation statements)

References 30 publications

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“…Natural compounds are an important source of new and selective drug prototypes for the treatment of tropical diseases caused by protozoans, such as leishmaniasis (Delorenzi & Attias, 2001;Valadares et al, 2011). The antileishmanial activity observed in total extracts prepared from these materials has been attributed to compounds belonging to diverse chemical groups, such as isoquinoline alkaloids, indole alkaloids, quinones, terpenes, steroids, carbohydrates, lignans, proteins, and flavonoids (Rocha et al, 2005;Marín et al, 2009;Valadares et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…The antileishmanial activity observed in total extracts prepared from these materials has been attributed to compounds belonging to diverse chemical groups, such as isoquinoline alkaloids, indole alkaloids, quinones, terpenes, steroids, carbohydrates, lignans, proteins, and flavonoids (Rocha et al, 2005;Marín et al, 2009;Valadares et al, 2011). Among these plant-derived products, flavonoids represent a large family of polyphenolic compounds found in vegetables and fruits.…”

Section: Discussionmentioning

confidence: 99%

“…After, parasites were washed two times in sterile PBS, and visualized in an optical light microscopy. The cellular density was estimated by counting in a Newbauer chamber, and their morphology was evaluated after staining by Giemsa (Valadares et al, 2011).…”

Section: Parasitementioning

confidence: 99%

“…Amphotericin B (1, 10 and 50 μ M) was used as a positive control. After fixation with 4% paraformaldehyde, cells were stained by Giemsa and observed in a light microscope to determine the percentage of infected macrophages, as well as the number of intramacrophage amastigotes out of 200 macrophages (Valadares et al, 2011). Data shown in this study represent the average ± standard deviation of three independent experiments, performed in triplicate.…”

Section: Treatment Of Infected Macrophagesmentioning

confidence: 99%

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Antileishmanial activity and evaluation of the mechanism of action of strychnobiflavone flavonoid isolated from Strychnos pseudoquina against Leishmania infantum

et al. 2015

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The present study aimed to investigate the in vitro antileishmanial activity of strychnobiflavone flavonoid against Leishmania infantum, as well as its mechanism of action, and evaluate the ex vivo biodistribution profile of the flavonoid in naive BALB/c mice. The antileishmanial activity (IC 50 value) of strychnobiflavone against stationary promastigote and amastigote-like stages of the parasites was of 5.4 and 18.9 μM, respectively; with a 50% cytotoxic concentration (CC 50 ) value of 125.0 μM on murine macrophages, resulting in selectivity index (SI) of 23.2 and 6.6, respectively. Amphotericin B, used as a positive control, presented SI values of 7.6 and 3.3 for promastigote and amastigote-like stages of L. infantum, respectively. The strychnobiflavone was also effective in reducing in significant levels the percentage of infected macrophages, as well as the number of amastigotes per macrophage, after the treatment of infected macrophages using the flavonoid. By using different fluorescent probes, we investigated the bioenergetics metabolism of L. infantum promastigotes and demonstrated that the flavonoid caused the depolarization of the mitochondrial membrane potential, without affecting the production of reactive oxygen species. In addition, using SYTOX ® green as a fluorescent probe, the strychnobiflavone demonstrated no interference in plasma membrane permeability. For the ex vivo biodistribution assays, the flavonoid was labeled with technetium99m and studied in a mouse model by intraperitoneal route. After a single dose administration, the scintigraphic images demonstrated a highest uptake by the liver and spleen of the animals within 60 min, resulting in low concentrations after 24 h. The present study therefore demonstrated, for the first time, the antileishmanial activity of the strychnobiflavone against L. infantum, and suggests that the mitochondria of the parasites may be the possible target organelle. The preferential distribution of this compound into the liver and spleen of the animals could warrant its employ in the treatment of visceral leishmaniasis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Parasitementioning

confidence: 99%

Section: Treatment Of Infected Macrophagesmentioning

confidence: 99%

See 2 more Smart Citations

Antileishmanial activity and evaluation of the mechanism of action of strychnobiflavone flavonoid isolated from Strychnos pseudoquina against Leishmania infantum

et al. 2015

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“…Antileishmanial drugs that are available in market, have raised questions due to their ineffectiveness, severe toxicity and side effects. 67 Mushroom and its metabolites are developing as costeffective alternative therapeutic strategy against Leishmania donovani.…”

Section: Antimicrobial and Antiparisitic Activitymentioning

confidence: 99%

A Comprehensive Review on Food and Medicinal Prospects of Astraeus hygrometricus

Biswas

Nandi²,

Kuila³

et al. 2017

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Proteomic Approaches to Identify Novel Therapeutics and Nutraceuticals from Filamentous Fungi

Banik¹,

Khowala

Pal

et al. 2015

Genomics, Proteomics and Metabolomics in Nutraceuticals and Functional Foods

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Leishmanicidal activity of the Agaricus blazei Murill in different Leishmania species

Cited by 76 publications

References 30 publications

Antileishmanial activity and evaluation of the mechanism of action of strychnobiflavone flavonoid isolated from Strychnos pseudoquina against Leishmania infantum

Antileishmanial activity and evaluation of the mechanism of action of strychnobiflavone flavonoid isolated from Strychnos pseudoquina against Leishmania infantum

A Comprehensive Review on Food and Medicinal Prospects of Astraeus hygrometricus

Proteomic Approaches to Identify Novel Therapeutics and Nutraceuticals from Filamentous Fungi

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