Length control is determined by the pattern of cytoskeleton

Kharitonova, Margarita A.; Vasiliev, J. M.

doi:10.1242/jcs.01054

Cited by 10 publications

(8 citation statements)

References 8 publications

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“…Having failed to identify a role for the actin cytoskeleton, these data prompted us to examine the function of microtubules, which have previously been implicated in cell spreading [20],[24]–[26],[35] and in the generation of an elongated cell shape [36],[37]. Strikingly, in the presence of the microtubule inhibitor colcemid [38], HeLa cells were unable to elongate (Figure 4A and 4D).…”

Section: Resultsmentioning

confidence: 99%

A Polarised Population of Dynamic Microtubules Mediates Homeostatic Length Control in Animal Cells

et al. 2010

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Section: Resultsmentioning

confidence: 99%

A Polarised Population of Dynamic Microtubules Mediates Homeostatic Length Control in Animal Cells

et al. 2010

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“…Many studies propose that actin polymerization traits are causative in the length control of cells as well as in contact guidance [ 14 , 36 , 37 ]. The authors conclude that cell orientation is determined by the formation of the actin cytoskeleton, whereas the elongation conforms to the orientation of actin fibers along the polarized cell axis.…”

Section: Discussionmentioning

confidence: 99%

“…Actin is the major cytoskeletal component and plays a central role in important cellular processes, e.g., in transduction of information in the outside-in signaling of cells by transmitting extracellular forces and tensions, and thus influencing the cell morphology [ 11 , 12 ]. In particular, many studies report that the arrangement of the actin cytoskeleton is decisive for cellular spreading as well as for the length control of cells [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Automatic Actin Filament Quantification of Osteoblasts and Their Morphometric Analysis on Microtextured Silicon-Titanium Arrays

et al. 2012

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Microtexturing of implant surfaces is of major relevance in the endeavor to improve biorelevant implant designs. In order to elucidate the role of biomaterial’s topography on cell physiology, obtaining quantitative correlations between cellular behavior and distinct microarchitectural properties is in great demand. Until now, the microscopically observed reorganization of the cytoskeleton on structured biomaterials has been difficult to convert into data. We used geometrically microtextured silicon-titanium arrays as a model system. Samples were prepared by deep reactive-ion etching of silicon wafers, resulting in rectangular grooves (width and height: 2 µm) and cubic pillars (pillar dimensions: 2 × 2 × 5 and 5 × 5 × 5 µm); finally sputter-coated with 100 nm titanium. We focused on the morphometric analysis of MG-63 osteoblasts, including a quantification of the actin cytoskeleton. By means of our novel software FilaQuant, especially developed for automatic actin filament recognition, we were first able to quantify the alterations of the actin network dependent on the microtexture of a material surface. The cells’ actin fibers were significantly reduced in length on the pillared surfaces versus the grooved array (4–5 fold) and completely reorganized on the micropillars, but without altering the orientation of cells. Our morpho-functional approach opens new possibilities for the data correlation of cell-material interactions.

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“…There were no significant differences in the cellular circularity between the multinucleated and mononuclear cells, and the cellular circularity appeared to be lower in the multinuclear cells. The cells treated with Y-27632 exhibited an increased degree of polarization and decreased circularity [21,22,23,24], or no change in cellular circularity [25]. Since Y-27632 was administered to obtain multinucleated cells in this study, the multinucleation of cells appears to decrease their circularity.…”

Section: Resultsmentioning

confidence: 99%

Multinucleation of Incubated Cells and Their Morphological Differences Compared to Mononuclear Cells

2019

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Some cells cultured in vitro have multiple nuclei. Since cultured cells are used in various fields of science, including tissue engineering, the nature of the multinucleated cells must be determined. However, multinucleated cells are not frequently observed. In this study, a method to efficiently obtain multinucleated cells was established and their morphological properties were investigated. Initially, we established conditions to quickly and easily generate multinucleated cells by seeding a Xenopus tadpole epithelium tissue-derived cell line (XTC-YF) on less and more hydrophilic dishes, and incubating the cultures with medium supplemented with or without Y-27632—a ROCK inhibitor—to reduce cell contractility. Notably, 88% of the cells cultured on a less hydrophilic dish in medium supplemented with Y-27632 became multinucleate 48 h after seeding, whereas less than 5% of cells cultured under other conditions exhibited this morphology. Some cells showed an odd number (three and five) of cell nuclei 72 h after seeding. Multinucleated cells displayed a significantly smaller nuclear area, larger cell area, and smaller nuclear circularity. As changes in the morphology of the cells correlated with their functions, the proposed method would help researchers understand the functions of multinucleated cells.

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Length control is determined by the pattern of cytoskeleton

Abstract: SummaryLength control is determined by the pattern of cytoskeleton

Cited by 10 publications

References 8 publications

A Polarised Population of Dynamic Microtubules Mediates Homeostatic Length Control in Animal Cells

A Polarised Population of Dynamic Microtubules Mediates Homeostatic Length Control in Animal Cells

Automatic Actin Filament Quantification of Osteoblasts and Their Morphometric Analysis on Microtextured Silicon-Titanium Arrays

Multinucleation of Incubated Cells and Their Morphological Differences Compared to Mononuclear Cells

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