Length-weight relationship (LWR) is likely the most useful tool to estimate indirect growth, body condition, and to understand the life cycle of fishes (Camara, Caramaschi, & Petry, 2011; Froese, 2006). Length and weight are biometrics data easily taken and available in most datasets from monitoring studies. Despite the simplicity, it provides highly useful information for fisheries management and can be used in different applications like the estimation of biomass from length data or comparisons between growth patterns and morphologic differentiation of the same species from different populations (Imorou, Alphonse, Edmond, & Youssouf, 2019; Karachle & Stergiou, 2012). The Amazon basin shelters a very highly diverse freshwater fish fauna that occupy a wide variety of environments. The middle stretch of the Amazon River sustains very important fishery activities, which targets about 100 species coming from its main channel and associated floodplain environments (Santos, Ferreira, & Zuanon, 2006). Here we present LWRs for 39 fish species based on 18 years of monthly fish sampling in the Catalão Lake, at the confluence of Amazonas and Negro rivers.