Lentil (<i>Lens culinaris</i> L.) growth promoting rhizobacteria and their effect on nodulation in coinoculation with rhizobia

Sepúlveda-Caamaño, Maurine; Gerding, Macarena; Vargas, Marisol; Moya-Elizondo, Ernesto A.; Oyarzúa, Pía; Campos, Jorge

doi:10.1080/03650340.2017.1342034

Cited by 26 publications

(14 citation statements)

References 51 publications

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“…Several PGPR have been isolated from different soils and crops in Central Chile and have shown that they can promote root and aerial growth, and increase nodule formation in legumes (Gerding et al, 2017;Sepúlveda-Caamaño et al, 2018;Cedeño-García et al, 2018). The aim of this study was to select rhizobacteria capable of improving NPK availability and promoting tomato growth.…”

Section: Researchmentioning

confidence: 99%

“…The PCR conditions were the following: one initial denaturation step at 95 °C for 5 min, followed by 30 cycles of denaturation at 94 °C for 30 s, 55 °C for 30 s and 72 °C for 90 s, and a final extension at 72 °C for 7 min. The amplification of the gene was verified through electrophoresis in agarose gel 1% (w/v), stained with GelRed (Biotium, Hayward, California, USA) at 90 V for 45 min (Sepúlveda-Caamaño et al, 2018).…”

Section: Identification Of Selected Bacterial Isolatesmentioning

confidence: 99%

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Plant growth-promoting rhizobacteria able to improve NPK availability: selection, identification and effects on tomato growth

Reyes-Castillo

Gerding²,

Oyarzúa³

et al. 2019

Chil. j. agric. res.

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Nitrogen, P and K are essential macronutrients that are not readily available to plants. Rhizobacteria are able to convert these unavailable forms for subsequent uptake by the plant, diverse species have been characterized as N2 fixers, P solubilizers and capable to solubilize mineral K from unavailable forms. The objective of this study was to select rhizobacteria capable of improving NPK availability and promoting tomato (Solanum lycopersicum L.) growth. Fifteen strains were studied. Four strains were selected for their capacity to fix N2, three for their ability to solubilize P, and six for their capacity to solubilize biotite and K-feldspar, isolated from tomato, lentil, chili pepper, faba bean and lettuce crops in Andisol and Alfisol soils. Through 16SrRNA sequencing, selected strains were identified as Pseudomonas gessardi, P. koreensis, P. brassicacearum, P. marginalis, Acinetobacter calcoaceticus and Rahnella aquatica. Phosphorus solubilizing strains did not show a positive effect on plant growth or an increase in available soil P. The N2 fixing bacteria Tmt-16 strain increased root growth in 23.57%; maintained the highest N content in plant tissue, 2.60%, higher amount of N available in the soil, 2.95 mg kg-1 , and a higher content of N-NH4 + 1.95 mg kg-1. The K solubilizing strains Ls-C21, Ltj-62 and LsC-58 reached 17.0 to 19.0 mg kg-1 available K and 0.04 to 0.05 mg kg-1 exchangeable K (p ≤ 0.05.). These four endemic rhizobacteria can be potentially used as biofertilizers, allowing a reduction in the use of chemical fertilizers and a more sustainable production of tomatoes.

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Section: Researchmentioning

confidence: 99%

Section: Identification Of Selected Bacterial Isolatesmentioning

confidence: 99%

Plant growth-promoting rhizobacteria able to improve NPK availability: selection, identification and effects on tomato growth

Reyes-Castillo

Gerding²,

Oyarzúa³

et al. 2019

Chil. j. agric. res.

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“…Bacterial strains of the genus Pseudomonas and Bacillus have been reported as plant growth promoters in alfalfa (Guiñazú et al, 2013;Kisiel and Kepczynska, 2016), with ability to synthesize IAA, to solubilize P and showing ACC deaminase activity (Rashid et al, 2012;Kisiel and Kepczynska, 2016). Since the sequencing of the 16S rRNA gene is not always sufficient to determine a species, because it is a slowly evolving gene compared to other housekeeping genes (Sepúlveda-Caamaño et al, 2018), sequencing of other conserved genes will be necessary to obtain the definitive identification.…”

Section: Identification Of Pgpr Strainsmentioning

confidence: 99%

“…Significant improvements in nodulation have been reported through inoculation with plant growth promoting rhizobacteria (PGPR) able to produce indole acetic acid (IAA) and with 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity (Rashid et al, 2012;Sepúlveda-Caamaño et al, 2018). The presence of ACC deaminase-producing bacteria in the root system reduces ethylene biosynthesis and increases the number of nodules, and produces changes in root architecture by promoting the development of lateral roots (Shahzad et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Plant growth promoting rhizobacteria with ACC deaminase activity isolated from Mediterranean dryland areas in Chile: Effects on early nodulation in alfalfa

Cedeño-García

Gerding²,

Moraga³

et al. 2018

Chil. j. agric. res.

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Alfalfa (Medicago sativa L. subsp. sativa) has emerged as an alternative crop for Mediterranean dryland areas in Chile. These soils show deficient nutritional levels affecting legume nodulation and N fixation through symbiosis with Ensifer meliloti. The objective of this work was to select plant growth promoting rhizobacteria (PGPR) associated with alfalfa and to evaluate their effects in plant growth and nodulation. Bacterial strains were isolated from the rhizosphere of alfalfa cultivated in dryland soil in Mediterranean areas of Chile. The 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, production of indole acetic acid (IAA), phosphate solubilization and the compatibility with E. meliloti was assessed for each strain. The selected bacterial strains were identified by the amplification of the 16S rRNA gene. Twelve of the 32 isolated strains were compatible with E. meliloti as they did not show signs of antagonism. Eight strains solubilized phosphate, 12 produced IAA ranging from 13.71 to 26.22 mg L-1 and only the GN-8 isolate reported ACC deaminase activity. In the plants, the nodulation was greater when the strain with ACC deaminase and E. meliloti were co-inoculated both in laboratory and greenhouse conditions (P < 0.05). The selected bacteria GN-2 and GN-8 belonged to the genus Bacillus, while GN-4 to Pseudomonas. In conclusion, PGPR collected in marginal soils of Mediterranean areas of Chile showed ACC deaminase activity, IAA production and phosphate solubilization. The co-inoculation with E. meliloti and Bacillus sp. GN-8 allowed earlier nodulation in alfalfa.

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“…Rhizotrons range in size from underground windowed facilities built under forests (Potvin and Lilleskov, ) to small plastic bags (i.e., seed germination pouches) used to study herbaceous plants in a laboratory setting (You et al., ). Researchers use rhizotrons to observe rhizobacteria‐associated phenotypes in legume roots including mutualist impacts (Sepúlveda‐Caamaño et al., ), nodulation formation and effectiveness (Yates et al., ), and parasitic plant resistance (Fernández‐Aparicio et al., ). Additional phenotypic applications include root ontogeny (Keng, ), environmental variation (Moser et al., ), and root architecture (Eberbach et al., ).…”

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confidence: 99%

Using clear plastic CD cases as low‐cost mini‐rhizotrons to phenotype root traits

et al. 2020

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Premise We developed a novel low‐cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to address the difficulties encountered growing plants in seed germination pouches for long‐term experiments and the high cost of other mini‐rhizotron alternatives. Methods and Results The method described here took inspiration from homemade ant farms commonly used as an educational tool in elementary schools. Using compact disc (CD) cases, we developed mini‐rhizotrons for use in the field and laboratory using the burclover Medicago lupulina. Conclusions Our method combines the benefits of pots and germination pouches. In CD mini‐rhizotrons, plants grew significantly larger than in germination pouches, and unlike pots, it is possible to measure roots without destructive sampling. Our protocol is a cheaper, widely available alternative to more destructive methods, which could facilitate the study of belowground phenotypes and processes by scientists with fewer resources.

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Lentil (Lens culinaris L.) growth promoting rhizobacteria and their effect on nodulation in coinoculation with rhizobia

Cited by 26 publications

References 51 publications

Plant growth-promoting rhizobacteria able to improve NPK availability: selection, identification and effects on tomato growth

Plant growth-promoting rhizobacteria able to improve NPK availability: selection, identification and effects on tomato growth

Plant growth promoting rhizobacteria with ACC deaminase activity isolated from Mediterranean dryland areas in Chile: Effects on early nodulation in alfalfa

Using clear plastic CD cases as low‐cost mini‐rhizotrons to phenotype root traits

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