Lentinoids A–D, New Natural Products Isolated from Lentinus strigellus

Abstract

Four novel lentinoids (1–4), along with the known compounds striguellone A (5), isopanepoxydone (6) and panepoxydone (7), were isolated as part of our studies on Lentinus strigellus. The structures of 1–4 have been established by 1D- and 2D-NMR and MS analysis. Compounds (1–3) and (5–7) were tested against Listeria monocytogenes, Enterococcus faecalis, Pseudomonas aeruginosa and Klebsiella pneumoniae. These compounds showed inhibition diameters ranging from 7.5–9.5 mm, however, when the minimum inhibitory concentration (MIC) was determined, only compound 1 showed a significant activity of 200 μg/mL. Intermediates for the biosynthesis of the oxygenated cyclohexenyl derivatives isolated from lentinoid fungi (genera Lentinus and Panus) are proposed.

Exploring the Antibacterial Activity of Pestalotiopsis spp. under Different Culture Conditions and Their Chemical Diversity Using LC–ESI–Q–TOF–MS

“…The solution was stored in the dark at 24 ± 2 °C. The bacterial inoculum of the seventeen pathogenic strains were prepared in Trypticase-Soy Agar (TSA; Bacto TM , Becton, Dickinson and Co., Sparks, MD, USA) for 24 h. Thereafter, five colonies were picked up and transferred into a tube containing a saline and isotonic solution, then visually compared to the turbidity standardas previously reported [ 54 ].…”

“…The turbidity standard (0.5 McFarland Turbidity Standard) was a BaCl 2 solution which absorbance (0.08-0.10 at 625 nm) was verified in a spectrophotometer (Spectronic 21, Bausch & Lomb, Rochester, NY, USA). The solution was stored in the dark at 24 ± 2 • C. The bacterial inoculum of the seventeen pathogenic strains were prepared in Trypticase-Soy Agar (TSA; Bacto TM , Becton, Dickinson and Co., Sparks, MD, USA) for 24 h. Thereafter, five colonies were picked up and transferred into a tube containing a saline and isotonic solution, then visually compared to the turbidity standardas previously reported [54].…”

Natural epoxyquinoids: isolation, biological activity and synthesis. An update

“…19). 84 Isopanepoxydone showed moderate antibacterial Organic & Biomolecular Chemistry Review activity against Listeria monocytogenes, Enterococcus faecalis and Pseudomonas aeruginosa at a concentration of 6.25 µg µL −1 . 4.1.10 Nectrianolin core.…”

A Mushroom P450‐Monooxygenase Enables Regio‐ and Stereoselective Biocatalytic Synthesis of Epoxycyclohexenones

“…In vitro evaluation of 1 showed potent inhibitory activity against the transcription factor NF‐κB as well as antitumor activity against various breast cancer cells in a dose‐ and time‐dependent manner [5,17] . In addition, isopanepoxydone, a 1 analogue, exhibited potent antimicrobial activity against Listeria monocytogenes , Enterococcus faecalis and Pseudomonas aeruginosa [16d] . Inspired by their significant bioactivities, the Wood group developed a synthetic strategy to stereoselectively synthesize 1 and its analogues from TBS‐protected bromoxone [18] …”