Leptin Modulates Neutrophil Phagocytosis of<i>Klebsiella pneumoniae</i>

Moore, Sharlene I.; Huffnagle, Gary B.; Chen, Gwo Hsiao; White, Eric S.; Mancuso, Peter

doi:10.1128/iai.71.7.4182-4185.2003

Cited by 95 publications

(79 citation statements)

References 22 publications

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“…An increased susceptibility to infections is exerted by functional abnormalities of PMN. Indeed, PMN from ob/ob mice showed impaired ability of phagocytosis that could be normalized by exogenous leptin administration [16]. These findings suggest that leptin is an immunomodulator for PMN function in rodents.…”

mentioning

confidence: 62%

Effects of Leptin and Tumor Necrosis Factor-.ALPHA. on Degranulation and Superoxide Production of Polymorphonuclear Neutrophils from Holstein Cows

et al. 2007

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ABSTRACT. Leptin, a pleiotropic hormone regulating food intake and energy expenditure, has been shown to directly modulate human polymorphonuclear neutrophil (PMN) functions or indirectly through the action of tumor necrosis factor-α (TNF-α). Bovine PMN have considerable different characteristics from human PMN. For example, it does not respond to N-formyl-Methionyl-Leucyl-phenylalanine, a well known human PMN activator. In the present study, we tested the effects of leptin and TNF-α on superoxide production and degranulation of bovine peripheral PMN, in which both long isoform of leptin receptor (Ob-Rb) and TNF receptor 1 were expressed. Human leptin, human TNF-α, phorbol myristate acetate (PMA) and opsonized zymosan particles (OZP) did not stimulate degranulation responses, while zymosan-activated serum (ZAS) did. Neither leptin nor TNF-α enhanced the ZAS-induced degranulation responses. TNF-α, PMA, OZP and ZAS increased superoxide production in different magnitudes, whereas leptin did not. TNF-α, but not leptin, enhanced OZP-and ZAS-induced superoxide production, possibly, in part due to facilitating translocation of p47 phox , a component of NADPH oxidase. These results indicate that, unlike in human PMN, leptin does not have any direct effect on degranulation and superoxide production in bovine PMN, although TNF-α influences superoxide production.

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confidence: 62%

Effects of Leptin and Tumor Necrosis Factor-.ALPHA. on Degranulation and Superoxide Production of Polymorphonuclear Neutrophils from Holstein Cows

et al. 2007

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“…5,6 Additionally, leptin deficiencies have been associated with impaired cell-mediated immunity and increased susceptibility to infections. [7][8][9][10] Leptin effects in the immune system include the promotion of T cell responses, activation of monocytes and neutrophils and the induction of pro-inflammatory mediators. 11 In macrophages, leptin acts as a potent chemoatractant and induces the production of inflammatory mediators including LTB 4 , TNFα and IL-6 among others.…”

Section: Leptin Modulation Of Immune Responsesmentioning

confidence: 99%

Leptin and mTOR: Partners in metabolism and inflammation

Maya‐Monteiro¹,

Bozza²

2008

Cell Cycle

102

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“…On the contrary, leptin is known to reduce expression of neutrophil chemoattractants and inhibits neutrophil migration in response to classical chemoattractants (41,42). Furthermore, leptin regulates functional capacities of neutrophils (11,12) and can protect neutrophils from apoptosis (43). Further investigations are required to unravel the role of leptin in neutrophil recruitment to the lungs in response to CS exposure.…”

Section: Figurementioning

confidence: 99%

“…Numerous treatment protocols are being used, varying in the dose of leptin per day (30-300 mg per animal per day), once or twice daily injections, treatment period (2 d to 6 wk), and origin (and thus biological activity) of leptin (12,(52)(53)(54)(55)(56), eventually resulting in a change in body weight of +3% to 225%. In our hands, leptin replacement therapy with a relatively low dose (45 mg) of murine leptin (E. coli) once daily for 4 wk maintained the initial body weight in C57BL/6 WT mice and air-exposed ob/ob mice, which is in line with several previous reports (52,(54)(55)(56)).…”

Section: Figurementioning

confidence: 99%

“…Recently published studies demonstrate that leptin has a potentiating role in the function of both innate and adaptive immunity (10), making it an ideal candidate for a role in chronic inflammatory airway diseases such as COPD. On neutrophils and macrophages, leptin stimulates chemotaxis (11) and enhances functional capacities, such as oxidative burst (11), phagocytosis (12), and cytokine secretion (13,14). In addition, leptin exerts proliferative (15) and antiapoptotic effects (16) on T lymphocytes and promotes Th1 cell differentiation (17).…”

mentioning

confidence: 99%

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Leptin Modulates Innate and Adaptive Immune Cell Recruitment after Cigarette Smoke Exposure in Mice

Vernooy¹,

Bracke

Drummen³

et al. 2010

The Journal of Immunology

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Leptin, a pleiotropic type I cytokine, was recently demonstrated to be expressed by resident lung cells in chronic obstructive pulmonary disease patients and asymptomatic smokers. To elucidate the functional role of leptin in the onset of chronic obstructive pulmonary disease, we tested leptin-deficient ob/ob mice (C57BL/6), leptin receptor-deficient db/db mice (C57BKS), and littermates in a model of cigarette smoke (CS)-induced pulmonary inflammation. Wild-type (WT) C57BL/6 mice were exposed for 4 or 24 wk to control air or CS. Pulmonary leptin expression was analyzed by immunohistochemistry and real-time PCR. Pulmonary inflammation upon 4 wk CS exposure was evaluated in bronchoalveolar lavage fluid (BALF) and lung tissue of WT, ob/ob, and db/db mice. Immunohistochemical analysis revealed leptin expression in bronchial epithelial cells, pneumocytes, alveolar macrophages, and bronchial/vascular smooth muscle cells. The 4 and 24 wk CS exposure increased leptin expression in bronchial epithelial cells and pneumocytes versus air-exposed WT mice (p < 0.05). The 4 wk CS exposure resulted in increased accumulation of neutrophils, dendritic cells, macrophages, and lymphocytes in BALF and lung tissue of WT, ob/ob, and db/db mice. CS-exposed ob/ob and db/db mice showed in general higher numbers of neutrophils and lower numbers of CD4+, CD8+, and dendritic cells versus CS-exposed WT mice. Consistently, CXCL1 levels were enhanced in BALF of CS-exposed ob/ob and db/db mice versus WT mice (p < 0.05). Exogenous leptin administration completely restored the skewed inflammatory profile in ob/ob mice. These data reveal an important role of leptin in modulating innate and adaptive immunity after CS inhalation in mice.

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Leptin Modulates Neutrophil Phagocytosis ofKlebsiella pneumoniae

Cited by 95 publications

References 22 publications

Effects of Leptin and Tumor Necrosis Factor-.ALPHA. on Degranulation and Superoxide Production of Polymorphonuclear Neutrophils from Holstein Cows

Effects of Leptin and Tumor Necrosis Factor-.ALPHA. on Degranulation and Superoxide Production of Polymorphonuclear Neutrophils from Holstein Cows

Leptin and mTOR: Partners in metabolism and inflammation

Leptin Modulates Innate and Adaptive Immune Cell Recruitment after Cigarette Smoke Exposure in Mice

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