Les entérocoques résistants aux glycopeptides

Cattoir, Vincent; Leclercq, Roland

doi:10.1051/medsci/20102611936

Cited by 11 publications

(6 citation statements)

References 24 publications

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“…VanA is the most frequent glycopeptide resistance type in clinical enterococci. Nowadays, there is an increasing concern about vanA plasmidmediated transfer to methicillin-resistant Staphylococcus aureus [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Pourcel¹,

Sparo²,

Alej³

et al. 2017

Clin Microbiol

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Enterococci often acquire antimicrobial resistance through horizontal gene transfer. Relatedness between enterococci with high level resistance to gentamicin and vancomycin isolated from humans, food and hospital environment in Tandil County (Argentina) was investigated. PCR amplification for species determination was carried out. Resistance to seven antimicrobials was studied; virulence genes (esp, cylA), vancomycin and gentamicin resistance genes were investigated. In the isolates with high level antimicrobial resistance (gentamicin, vancomycin), pulse-field gel electrophoresis was performed. Vancomycin-resistant E. faecium (n:13) were recovered from human, food and hospital environment samples. All the isolates expressed high-level vancomycin and teicoplanin (vanA genotype), as well high-level gentamicin and streptomycin resistance. Vancomycin-resistant E. faecium were distributed among seven clonal types; esp gene was detected in clinical strains. There was no clonal relationship with food vanA E. faecium, but these strains could pose a risk in intra/inter genus transfer of vanA determinant to human-adapted strains. High-level gentamicin resistant E. faecalis (n:7) were recovered from human and food samples. Glycopeptide resistance was not observed; cylA gene was detected in most of the clinical high-level gentamicin resistant E. faecalis isolates. PFGE patterns showed four clonal types in high-level gentamicin resistant E. faecalis strains; there was demonstrated clonal relatedness between isolates from different origin. In Argentina, this is the first study showing a clonal relationship between high-level gentamicin resistant E. faecalis isolated from food and humans. These results encourage the study of dissemination of clonal complexes with mobile resistance genes.

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Section: Introductionmentioning

confidence: 99%

Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Pourcel¹,

Sparo²,

Alej³

et al. 2017

Clin Microbiol

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“…In the past two decades, the emergence of vancomycin‐resistant enterococci (VRE) caused concern in public health as vancomycin is, in some cases, one of the few therapeutic alternatives for treatment of enterococcal infections; furthermore, the risk of transfer of vancomycin resistance genes from enterococci to other pathogenic bacteria such as methicillin‐resistant Staphylococcus aureus is also a worrisome issue. VRE were firstly reported in human clinical setting at the end of the 1980, and since then, VRE have been found all over the world (Cattoir and Leclercq, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Ten different genotypes of glycopeptide resistance have been described in enterococci of either acquired ( vanA , vanB , vanD , vanE , vanG, vanL, vanN and vanM ) or intrinsic type ( vanC1 and vanC2/vanC3, associated with Enterococcus gallinarum and Enterococcus casseliflavus/flavescens species, respectively) (Cattoir and Leclercq, 2010), being the vanA and vanB genotypes the most frequently detected and worrisome ones in the clinical setting.…”

Section: Introductionmentioning

confidence: 99%

Study of Vancomycin Resistance in Faecal Enterococci from Healthy Humans and Dogs in Spain a Decade after the Avoparcin Ban in Europe

López¹,

Tenorio²,

Torres³

2012

Zoonoses and Public Health

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One hundred and 26 faecal samples from healthy dogs (2009) and 157 faecal samples from healthy humans (2007) from La Rioja region (Spain) were tested to know the carriage of vancomycin-resistant enterococci (VRE). VRE with intrinsic resistance (vanC) were found in 12% of healthy dogs and humans (29 Enterococcus gallinarum and four Enterococcus casseliflavus). Nevertheless, VRE with acquired mechanism of resistance were not detected among these samples. Four Enterococcus faecalis isolates with vancomycin MIC of 8-16 mg L(-1) were recovered in human samples, but no single organism with known mechanism of acquired resistance could be identified. These 37 VRE isolates (33 E. gallinarum/E. casseliflavus and four E. faecalis) of dog and human origin were further characterized (PCR detection of antibiotic resistance, virulence and bacteriocin genes). High prevalence of tetracycline resistance was identified (70%), especially among dog isolates harbouring tet(M) ± tet(L) genes; erythromycin resistance was also higher among isolates from dogs and they harboured the erm(B) gene, associated with erm(A) gene in one case. Virulence genes were only identified among E. faecalis isolates of human origin (agg, cpd and/or gelE) and never among E. gallinarum/E. casseliflavus of human or dog origin. Five E. gallinarum isolates of dog and three E. faecalis of human origin expressed bacteriocin activity; among them, only one E. faecalis presented activity against Listeria monocytogenes. The bacteriocin structural gene ef1097 was identified in 3 bacteriocin-producing E. faecalis isolates, associated with ent1071 in one of them.

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“…Ces souches hébergent en effet une sous-population résistante aux glycopeptides. Parmi les isolats de GISA responsables d'infections cliniques, aucun ne dispose de gènes tels que vanA, vanB, vanC1, vanC2, vanC3 associés à la résistance aux glycopeptides chez les entéroco-ques [33] (➜). En effet, l'acquisition du gène vanA à l'origine des S. aureus résistants à la vancomycine (VRSA) est un phénomène très rare et, actuellement, seuls 9 isolats cliniques VRSA ont été décrits dans le monde.…”

Section: Mécanisme De Résistanceunclassified