2000
DOI: 10.1016/s0166-0934(99)00160-3
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Lessons from a multicentre study of the detectability of viral genomes based on a two-round quality control of GB virus C (GBV-C)/hepatitis G virus (HGV) polymerase chain reaction assay

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Cited by 12 publications
(12 citation statements)
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“…A multicentre study on molecular detection of GBV-C displayed a wide range of sensitivity and specificity achieved among participating laboratories 7 . Primer selection is a key point in the development of successful PCR strategies.…”
Section: Discussionmentioning
confidence: 99%
“…A multicentre study on molecular detection of GBV-C displayed a wide range of sensitivity and specificity achieved among participating laboratories 7 . Primer selection is a key point in the development of successful PCR strategies.…”
Section: Discussionmentioning
confidence: 99%
“…Consistent with this finding, three studies involving more than 20 laboratories evaluating the reproducibility of GBV-C RNA testing found surprisingly heterogeneous results, even among laboratories using a commercial assay (5,12,13). Because different testing methods for GBV-C viremia may influence both the estimated prevalence and the estimates of associations between GBV-C and HIV disease markers, we studied three populations of HIV-infected individuals with several primer sets in order to determine the optimal approach for detecting GBV-C viremia.…”
mentioning
confidence: 66%
“…In addition, the use of nested RT-PCR for amplification of clinical samples is known to have problems with PCR carryover, leading to false-positive results. Thus, it is not too surprising that previous quality assurance studies (5,12,13) found poor test Positive 51 2 43 11 4 94 13 4 Negative 81 41 80 82 3 161 123 3 Total 132 43 123 93 7 255 136 7 a QNS, quantity not sufficient for testing.…”
Section: Discussionmentioning
confidence: 96%
“…Several assays have been used for GBV-C/HGV detection [8,9,12,18,19,21], and genomic variability, together with the type of biological specimen, extraction methods, primer selection, and analytical performances (sensitivity and specificity) of the assays are still partially unresolved technical issues, as evidenced in multicenter quality control trials [20,27].…”
Section: Discussionmentioning
confidence: 99%