Lessons from Studies of<i>Symbiobacterium</i><i>thermophilum</i>, a Unique Syntrophic Bacterium

Uéda, Kenji; Beppu, Teruhiko

doi:10.1271/bbb.60727

Cited by 29 publications

(21 citation statements)

References 22 publications

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“…Only six families are present in all genomes at T 25 , only two are present in all genomes at T 30 , one at T 35 , and none are present in all genomes at T 40 and higher. Rather, the clique results from the high connectivity of gene-sharing patterns for 54,349 to 66,118 (T 25 to T 40 ) families distributed among 181 genomes ranging in size from 307 to 4,820 families each, with a mean of 2,133 Ϯ 1,252 at T 30 .…”

Section: Modules and Community Structure In Network Of Shared Genesmentioning

confidence: 99%

Modular networks and cumulative impact of lateral transfer in prokaryote genome evolution

Dagan

Artzy‐Randrup

Martin

2008

Proc. Natl. Acad. Sci. U.S.A.

354

339

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Lateral gene transfer is an important mechanism of natural variation among prokaryotes, but the significance of its quantitative contribution to genome evolution is debated. Here, we report networks that capture both vertical and lateral components of evolutionary history among 539,723 genes distributed across 181 sequenced prokaryotic genomes. Partitioning of these networks by an eigenspectrum analysis identifies community structure in prokaryotic gene-sharing networks, the modules of which do not correspond to a strictly hierarchical prokaryotic classification. Our results indicate that, on average, at least 81 ؎ 15% of the genes in each genome studied were involved in lateral gene transfer at some point in their history, even though they can be vertically inherited after acquisition, uncovering a substantial cumulative effect of lateral gene transfer on longer evolutionary time scales.community structure ͉ molecular phylogeny ͉ microbial genomes

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Section: Modules and Community Structure In Network Of Shared Genesmentioning

confidence: 99%

Modular networks and cumulative impact of lateral transfer in prokaryote genome evolution

Dagan

Artzy‐Randrup

Martin

2008

Proc. Natl. Acad. Sci. U.S.A.

354

339

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“…These two components also showed different biochemical properties such as molecular weight, heat stability and proteinase sensitivity [20]. Recently, Ueda et al suggested a possibility of syntrophic relationship between S. thermophilum and Bacillus strain S [30]. They focused carbonic anhydrase of the helper bacterium which reversibly converts CO 2 to carbonate ion because no homolog of carbonic anhydrase was observed in genome sequence of S. thermophilum .…”

Section: Discussionmentioning

confidence: 99%

A commensal symbiotic interrelationship for the growth of Symbiobacterium toebii with its partner bacterium, Geobacillus toebii

et al. 2011

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BackgroundSymbiobacterium toebii is a commensal symbiotic thermophile that absolutely requires its partner bacterium Geobacillus toebii for growth. Despite development of an independent cultivation method using cell-free extracts, the growth of Symbiobacterium remains unknown due to our poor understanding of the symbiotic relationship with its partner bacterium. Here, we investigated the interrelationship between these two bacteria for growth of S. toebii using different cell-free extracts of G. toebii.ResultsSymbiobacterium toebii growth-supporting factors were constitutively produced through almost all growth phases and under different oxygen tensions in G. toebii, indicating that the factor may be essential components for growth of G. toebii as well as S. toebii. The growing conditions of G. toebii under different oxygen tension dramatically affected to the initial growth of S. toebii and the retarded lag phase was completely shortened by reducing agent, L-cysteine indicating an evidence of commensal interaction of microaerobic and anaerobic bacterium S. toebii with a facultative aerobic bacterium G. toebii. In addition, the growth curve of S. toebii showed a dependency on the protein concentration of cell-free extracts of G. toebii, demonstrating that the G. toebii-derived factors have nutrient-like characters but not quorum-sensing characters.ConclusionsNot only the consistent existence of the factor in G. toebii during all growth stages and under different oxygen tensions but also the concentration dependency of the factor for proliferation and optimal growth of S. toebii, suggests that an important biosynthetic machinery lacks in S. toebii during evolution. The commensal symbiotic bacterium, S. toebii uptakes certain ubiquitous and essential compound for its growth from environment or neighboring bacteria that shares the equivalent compounds. Moreover, G. toebii grown under aerobic condition shortened the lag phase of S. toebii under anaerobic and microaerobic conditions, suggests a possible commensal interaction that G. toebii scavengers ROS/RNS species and helps the initial growth of S. toebii.

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“…isolated in this study: 1 Â 10 4 cells per ml of S. thermophilum and Ureibacillus sp. each were simultaneously inoculated into 100 ml of T2 liquid medium containing (g per liter) tryptone peptone (2) and NaCl (0.5), pH 7.5, in a 300-ml Erlenmeyer flask and cultured in a stationary state at 60 C for 6 d. The growth of S. thermophilum was measured by quantitative PCR (see below).…”

Section: Methodsmentioning

confidence: 99%

“…1,2) This organism is characterised by growth dependence on coculture with a cognate Geobacillus stearothermophilus strain S (formerly Bacillus sp. strain S; see below), which was simultaneously isolated from the compost.…”

Section: Distribution Of Symbiobacterium Thermophilum and Related Bacmentioning

confidence: 99%

Distribution ofSymbiobacterium thermophilumand Related Bacteria in the Marine Environment

Sugihara¹,

Watsuji²,

Kubota³

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Lessons from Studies ofSymbiobacteriumthermophilum, a Unique Syntrophic Bacterium

Cited by 29 publications

References 22 publications

Modular networks and cumulative impact of lateral transfer in prokaryote genome evolution

Modular networks and cumulative impact of lateral transfer in prokaryote genome evolution

A commensal symbiotic interrelationship for the growth of Symbiobacterium toebii with its partner bacterium, Geobacillus toebii

Distribution ofSymbiobacterium thermophilumand Related Bacteria in the Marine Environment

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