Lethal Effects of Helianthemum lippii (L.) on Acanthamoeba castellanii Cysts in Vitro

Badria, Farid A.; Hetta, Mona H.; Sarhan, Rania M.; El-Din, M.H. Ezz

doi:10.3347/kjp.2014.52.3.243

Cited by 14 publications

(12 citation statements)

References 30 publications

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“…Trophozoites feed on bacteria, algae or yeast or grow axenically on nutrients [ 1 ]. Encystment occurs when conditions become unfavorable, such as during lack of nutrients or desiccation and cysts are highly resistant to temperature and pH changes [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities

et al. 2018

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Poly (dl-lactide-co-glycolide) (PLGA) microspheres were synthesized as delivery system for the natural anti-parasitic compounds, Periglaucine A (PGA) and Betulinic acid (BA). Periglaucine A and Betulinic acid were encapsulated in PLGA nanoparticles by single emulsion method with an average particle size of approximately 100–500 nm. Periglaucine A and Betulinic acid encapsulation efficiency was observed to be 90% and 35% respectively. Anti-Acanthamoeba property of Periglaucine A and Betulinic acid remained intact after encapsulation. PGA-PLGA and BA-PLGA nanoparticles demonstrated inhibition in viability of Acanthamoeba triangularis trophozoites by 74.9%, 59.9%, 49.9% and 71.2%, 52.2%, 88% respectively at concentration of 100 µg/mL, 50 µg/mL and 25 µg/mL. Cytotoxicity of PGA-PLGA and BA-PLGA nanoparticles has been evaluated against lung epithelial cell line and showed dose dependent cytotoxicity value of IC50 2 µg/mL and 20 µg/mL respectively. Futher, increased viability was observed in lung epithelial cell line in higher doses of synthesized polymeric nanoparticles. Results indicate that poly (dl-lactide-co-glycolide) (PLGA) nanoparticles could be exploratory delivery systems for natural products to improve their therapeutic efficacy.

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Section: Introductionmentioning

confidence: 99%

Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities

et al. 2018

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“…However, the tested plants (Arachis hypogaea, Curcuma longa, Pancratium maritimum) showed higher growth inhibitory percentages up to 100% by their maximum effective tested doses. In another in vitro study, ethyl acetate and methanol extract of Helianthemum lippii showed higher lethal effect against Acanthamoeba castellanii cysts than chlorhexidine (Badria et al 2014). Also, Ismail et al (2018) stated that different concentrations of both aqueous and alcoholic extracts of Nigella sativa possessed more potent cysticidal effect on Acanthamoeba cysts with growth inhibition up to 100% compared to chlorhexidine 0.02% which gave growth inhibitory effect ranging from 56 to 80% throughout the experiment.…”

Section: Discussionmentioning

confidence: 97%

In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts

et al. 2020

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This study evaluated in vitro effect of different concentrations of Aloe vera (A. vera) ethanol extract and honey against Acanthamoeba spp. cysts in comparison with chlorhexidine (the drug of choice for treatment of Acanthamoeba infection) at different incubation periods. Four different concentrations of the tested agents were used, 100, 200, 400, and 600 lg/ml for A. vera ethanol extract and 25, 50, 100, and 200 lg/ml for honey. Isolated Acanthamoeba spp. cysts from keratitis patients were incubated with different concentrations of the tested agents as well as chlorhexidine 0.02% (drug control) for different incubation periods (24, 48, 72 h). After each incubation period, the effect of A. vera extract and honey against Acanthamoeba cysts was assessed by counting the number of viable cysts, determining the inhibitory percentage and detecting the morphological alternations of treated cysts compared to non-treated and drug controls. Both A. vera ethanol extract and honey showed a concentration and time-dependent effect on the viability of Acanthamoeba cysts. In comparison with chlorhexidine (the drug control), A. vera ethanol extract possessed a potent cysticidal activity at all tested concentrations throughout different incubation periods, except for concentration 100 lg/ml which recorded the lower inhibitory effect. With increasing the dose of A. vera ethanol extract to 200, 400, 600 lg/ml, the recorded inhibitory percentages of Acanthamoeba cysts viability were 82.3%, 92.9% and 97.9% respectively, after 72 h compared to 76.3% of chlorhexidine. Similarly, honey at concentrations of 50-100 lg/ml gave higher inhibitory effect of 59% and 76.7%, respectively compared to chlorhexidine which showed an inhibitory percentage of 55.7% after 24 h. Meanwhile, the lowest tested concentration of honey (25 lg/ml) gave an inhibitory effect by 47.7-67% which was less than that of chlorhexidine throughout different incubation periods. With increasing the dose of honey to 200 lg/ml, the inhibitory effect was 98.9% after 72 h higher than that of chlorhexidine (76.9%). Using a scanning electron microscope, Acanthamoeba cysts treated by A. vera ethanol extract showed alternations in their shapes with flattening, collapsing, and laceration of their walls. Also, treated cysts by honey were highly distorted and difficult to identify because most of them were shrinkage and collapsed to a tiny size. On the other hand, chlorhexidine showed less structural and morphological changes of Acanthamoeba cysts. A. vera ethanol extract and honey had considerable cysticidal effects on Acanthamoeba cysts. They may give promising results for treatment of Acanthamoeba keratitis.

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“…In the same year, Shoaib et al [47] reported the activities of methanolic plant extracts on the viability of Acanthamoeba castellanii (T4 genotype) and its cytotoxic efficacy on human corneal epithelial cells. The plant crude extracts (maximum dose, 1.5 mg/ml) inhibited and killed > 50% of trophozoites within 1 h. In vitro amoebicidial activity of Helianthemum lippii (L.) methanol and ethyl acetate extracts on Acanthamoeba castellanii cysts was represented by Badria et al [3]. Although both extracts demonstrated potent lethal activity on A. castellanii cysts, the ethyl acetate extract was more effective than the methanol extract.…”

Section: Discussionmentioning

confidence: 97%

Amoebicidal and Amoebistatic Effects of Artemisia argyi Methanolic Extracts on Acanthamoeba castellanii Trophozoites and Cysts

Kolören

Sekeroglu

et al. 2019

Acta Parasit.

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Purpose The present study aimed to investigate the amoebicidal and amoebistatic efects of Artemisia argyi leaf methanolic extract by testing the effects on trophozoites and on cysts. We also determined cytotoxic effect, enzymatic and non-enzymatic antioxidant activities, total phenolic, lavonoid and antioxidative contents of A. argyi. Methods A. argyi was harvested from various geographic sites in Ordu province in Turkey. The fresh leaves were subjected to methanolic extraction. In 100 μl culture, different concentrations of A. argyi methanolic extract (in quantities from 1.2, 2.3, 4.7, 9.4, 18.7, 37.4, 74.8 mg/ml) and the same volume of trophozoite/cyst suspension were mixed for the determination of the amoebicidal activity of the plant extract. Human bronchial epithelial cells were treated with the same concentrations of Artemisia extracts to determine cytotoxic potential. Results Total phenolic and lavonoid contents of the extract were calculated as 261 mg gallic acid/g dry extract and 29 mg quercetin/g dry extract, respectively. Total antioxidant activity was also calculated as 367 mg ascorbic acid/g dry extract.The growth of trophozoites stopped in A. argyi methanolic extract with 50% inhibitory concentrations (IC50)/8 h for 37.4 mg/ ml and 74.8 mg/ml extract solution and had stronger amoebicidal activity on the cysts with IC50/72 h. Artemisia showed stronger inhibitory effects on bronchial epithelial cells at the concentrations of 9.4, 18.7, 37.4 and 74.8 mg/ml. ConclusionThe study indicated that A. argyi leaf extract has cytotoxic and anti-amoebic activities.

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Lethal Effects of Helianthemum lippii (L.) on Acanthamoeba castellanii Cysts in Vitro

Cited by 14 publications

References 30 publications

Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities

Preparation of Poly (dl-Lactide-co-Glycolide) Nanoparticles Encapsulated with Periglaucine A and Betulinic Acid for In Vitro Anti-Acanthamoeba and Cytotoxicity Activities

In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts

Amoebicidal and Amoebistatic Effects of Artemisia argyi Methanolic Extracts on Acanthamoeba castellanii Trophozoites and Cysts

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