Letters to the Editor

Crawford, Colin L.; Sabin, Thomas D.; Dyro, Frances M.; Swift, Thomas R.; Rivner, Michael H.; Gulati, Adarsh K.

doi:10.1002/mus.880110314

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Our summary of findings in this study suggests that CSF cytotoxicity is a feature of sporadic and also possibly of familial ALS, with ALS–CSF appearing to exert cytotoxicity at a wide range of concentrations and exposure times, as well as across different culture models. Although a few studies failed to observe cytotoxicity following exposure to ALS–CSF compared to controls, we note that these were generally earlier studies ( Askanas et al , 1981 ; Silani et al , 1987 ; Crawford et al , 1988 ; Iwasaki et al , 1995 ). Our results also indicate an apoptotic component to ALS–CSF, with greater apoptosis being observed in cells following exposure to CSF from patients with both ALS and FTD ( Ding et al , 2015 ).…”

Section: Discussionsupporting

confidence: 49%

“…Another study also failed to observe a decrease in neuronal survival at a 24-h time point, but only reported the difference as significant on day 10 ( Silani et al , 1987 ). Three early studies did not find any significant difference in cytotoxicity following exposure to ALS–CSF compared to controls ( Askanas et al , 1981 ; Crawford et al , 1988 ; Iwasaki et al , 1995 ). Two studies also assessed cytotoxicity within ALS patient populations.…”

Section: Resultsmentioning

confidence: 95%

See 1 more Smart Citation

Cerebrospinal fluid cytotoxicity in amyotrophic lateral sclerosis: a systematic review of in vitro studies

Kwong

Gregory

Pal

et al. 2020

Brain Communications

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Various studies have suggested that a neurotoxic cerebrospinal fluid (CSF) profile could be implicated in amyotrophic lateral sclerosis (ALS). Here, we systematically review the evidence for CSF cytotoxicity in ALS and explore its clinical correlates. We searched the following databases with no restrictions on publication date: PubMed, Embase and Web of Science. All studies that investigated cytotoxicity in vitro following exposure to CSF from ALS patients were considered for inclusion. Meta-analysis could not be performed, and findings were instead narratively summarised. 28 studies were included in our analysis. Both participant characteristics and study conditions including CSF concentration, exposure time and culture model varied considerably across studies. Of 22 studies assessing cell viability relative to controls, 19 studies reported a significant decrease following exposure to ALS-CSF, while three early studies failed to observe any difference. Seven of eight studies evaluating apoptosis observed significant increases in the levels of apoptotic markers following exposure to ALS-CSF, with the remaining study reporting a qualitative difference. Although five studies investigated the possible relationship between CSF cytotoxicity and patient characteristics, such as age, gender and disease duration, none demonstrated an association with any of the factors. In conclusion, our analysis suggests that CSF cytotoxicity is a feature of sporadic and possibly also of familial forms of ALS. Further research is, however, required to better characterise its underlying mechanisms and to establish its possible contribution to ALS pathophysiology.

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