Leucine-Rich Immune Factor APL1 Is Associated With Specific Modulation of Enteric Microbiome Taxa in the Asian Malaria Mosquito Anopheles stephensi

Mitri, Christian; Bischoff, Emmanuel; Volant, Stevenn; Ghozlane, Amine; Eiglmeier, Karin; Holm, Inge; Dieme, Constentin; Brito-Fravallo, Emma; Guelbeogo, Wamdaogo M.; Sagnon, N’Falé; Riehle, Michelle M.; Vernick, Kenneth D.

doi:10.3389/fmicb.2020.00306

Cited by 20 publications

(27 citation statements)

References 79 publications

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“…Microbiome assembly in mosquitoes is influenced by the environment, host and bacterial genetics, and stochastic processes. While the host is instrumental in maintaining microbiome homeostasis [10][11][12][13][14], evidence is emerging that bacterial genetics and microbe-microbe interactions also dictate the prevalence and abundance of microbiota [15][16][17][18]. These processes are important as the microbiome can influence the ability of mosquitoes to transmit pathogens [3,19,20], but potentially other traits related to vectorial capacity.…”

Section: Introductionmentioning

confidence: 99%

Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity

et al. 2020

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How microbe–microbe interactions dictate microbial complexity in the mosquito gut is unclear. Previously we found that, Serratia, a gut symbiont that alters vector competence and is being considered for vector control, poorly colonized Aedes aegypti yet was abundant in Culex quinquefasciatus reared under identical conditions. To investigate the incompatibility between Serratia and Ae. aegypti, we characterized two distinct strains of Serratia marcescens from Cx. quinquefasciatus and examined their ability to infect Ae. aegypti. Both Serratia strains poorly infected Ae. aegypti, but when microbiome homeostasis was disrupted, the prevalence and titers of Serratia were similar to the infection in its native host. Examination of multiple genetically diverse Ae. aegypti lines found microbial interference to S. marcescens was commonplace, however, one line of Ae. aegypti was susceptible to infection. Microbiome analysis of resistant and susceptible lines indicated an inverse correlation between Enterobacteriaceae bacteria and Serratia, and experimental co-infections in a gnotobiotic system recapitulated the interference phenotype. Furthermore, we observed an effect on host behavior; Serratia exposure to Ae. aegypti disrupted their feeding behavior, and this phenotype was also reliant on interactions with their native microbiota. Our work highlights the complexity of host–microbe interactions and provides evidence that microbial interactions influence mosquito behavior.

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Section: Introductionmentioning

confidence: 99%

Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity

et al. 2020

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“…In line with these reports, silencing of TEP1 also affected midgut microbiota by an as yet unknown mechanism [ 24 ]. Furthermore, depletion of APL1 in As resulted in altered midgut microbiome, a change that could affect parasite invasion [ 25 ]. Our findings extend these observations to the early stages of parasite invasion and suggest that in addition to parasite killing at the basal side, TEP1 directly or indirectly inhibits Plasmodium midgut traversal.…”

Section: Resultsmentioning

confidence: 99%

Kinetics of Plasmodium midgut invasion in Anopheles mosquitoes

et al. 2020

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Malaria-causing Plasmodium parasites traverse the mosquito midgut cells to establish infection at the basal side of the midgut. This dynamic process is a determinant of mosquito vector competence, yet the kinetics of the parasite migration is not well understood. Here we used transgenic mosquitoes of two Anopheles species and a Plasmodium berghei fluorescence reporter line to track parasite passage through the mosquito tissues at high spatial resolution. We provide new quantitative insight into malaria parasite invasion in African and Indian Anopheles species and propose that the mosquito complement-like system contributes to the species-specific dynamics of Plasmodium invasion.

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“…Environmental sources of Cedecea constituting potential reservoirs for infection are numerous. Similar to other genera in the Enterobacteriaceae family, Cedecea species have been detected in diverse ecological niches, including aquatic habitats (Chun et al, 2017;Chan and Tan, 2017), soil or agricultural dusts (Pande et al, 2000), plants (Akinsanya et al, 2015), retail food (Chan et al, 2014;Ye et al, 2017), insect vectors (Hegde et al, 2019;Mitri et al, 2020), human gut microbiome (Human Microbiome Project Consortium, 2012), and non-human animals (Boath et al, 2020;Kateete et al, 2013). In a case of leg ulcer infection and bacteremia, Dalamaga et al (2008b) speculated that transmission of the C. davisae pathogen occurred from direct exposure of the patient's minor leg lesion with lake water prior to hospitalization.…”

Section: Environmental Distribution and Epidemiologymentioning

confidence: 99%

Expanding spectrum of opportunistic Cedecea infections: Current clinical status and multidrug resistance

Thompson

Sharkady

2020

International Journal of Infectious Diseases

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Members of the bacterial genus Cedecea cause acute infections worldwide in compromised hosts with serious underlying medical conditions. While global reports of Cedecea infections remain sporadic in the medical literature, cases of multidrug-resistant clinical isolates have been documented each year over the past decade, warranting a comprehensive update on this emerging opportunistic pathogen. Here, we review the clinical manifestations, pathogenesis, natural distribution, epidemiology, and antimicrobial resistance of Cedecea species. Acute infection commonly manifests as bacteremia and pneumonia; however, the spectrum of infectious pathologies associated with Cedecea has expanded to include oral and cutaneous ulcers, orbital cellulitis, and peritonitis. The frequency of resistance among reported clinical isolates was highest to ampicillin, cephalothin, cefoxitin, cefazolin, and ceftazidime. Cedecea isolates harboring metallo-β-lactamases exhibited resistance to carbapenems and fourth-generation cephalosporins. Further research is needed to understand the pathogenicity and multidrug resistance of Cedecea species. Appropriate therapeutic management of Cedecea infections depends on antibiotic susceptibility testing because of variable resistance patterns and the enhanced infection risk in vulnerable populations.

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Leucine-Rich Immune Factor APL1 Is Associated With Specific Modulation of Enteric Microbiome Taxa in the Asian Malaria Mosquito Anopheles stephensi

Cited by 20 publications

References 79 publications

Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity

Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity

Kinetics of Plasmodium midgut invasion in Anopheles mosquitoes

Expanding spectrum of opportunistic Cedecea infections: Current clinical status and multidrug resistance

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