Leukemia inhibitory factor increases the invasiveness of trophoblastic cells through integrated increase in the expression of adhesion molecules and pappalysin 1 with a concomitant decrease in the expression of tissue inhibitor of matrix metalloproteinases

Oncostatin M and leukaemia inhibitory factor trigger signal transducer and activator of transcription 3 and extracellular signal-regulated kinase 1/2 pathways but result in heterogeneous cellular responses in trophoblast cells

“…It can be argued that the concentrations used in the present study were lower than in other studies; for example, Suman et al (2013) found that 50 ng mL À1 LIF increased the invasion of HTR-8/SVneo cells. Nevertheless, the concentrations used in the present study enhanced the invasiveness of the choriocarcinoma-derived cell lines JEG-3 and ACH-3P, which is in line with a previous report (Morales-Prieto et al 2013).…”

Leukemia Inhibitory Factor: Roles in Embryo Implantation and in Nonhormonal Contraception

“…LIF induces trophoblast cell proliferation via stimulating cell transition into G(2)/M phase of the cell cycle and activates both STAT3 and ERK1/2 signaling cascades [94]. Recently, LIF has been shown to increase invasiveness of human trophoblast cell line (HTR-8/SVneo cells) in vitro via STAT1 and STAT3 activation [95] as well as increase invasiveness of extravillous trophoblast cells via stimulating adhesion to the extracellular matrix elements including fibronectin, vitronectin, and laminin [96]. On the other hand, LIF was also reported to downregulate the expression of genes that encode TIMP1, TIMP2, and TIMP3 [95], therefore helping to reduce the expression of enzymes that are involved in potentiating trophoblast invasion.…”

“…Recently, LIF has been shown to increase invasiveness of human trophoblast cell line (HTR-8/SVneo cells) in vitro via STAT1 and STAT3 activation [95] as well as increase invasiveness of extravillous trophoblast cells via stimulating adhesion to the extracellular matrix elements including fibronectin, vitronectin, and laminin [96]. On the other hand, LIF was also reported to downregulate the expression of genes that encode TIMP1, TIMP2, and TIMP3 [95], therefore helping to reduce the expression of enzymes that are involved in potentiating trophoblast invasion. LIF was also reported to decrease the expression of integrin β 4 mRNA in the trophoblast cells which promotes trophoblast invasion [96].…”

STAT3 and ERK1/2 Cross-talk in Leukaemia Inhibitory Factor Mediated Trophoblastic JEG-3 Cell Invasion and Expression of Mucin 1 and Fos

“…1 Among others, leukaemia inhibitory factor (LIF) is one of the cytokines that increases the invasiveness of trophoblastic cells under in vitro conditions. 2,3 In extravillous trophoblastic (HTR-8/SVneo) cells, LIF increases their invasiveness by activating STAT and ERK1/2-dependent signaling pathways. 2 Silencing of STAT3 expression in trophoblastic JEG-3 cells led to a decrease in LIF-mediated increase in invasiveness.…”

“…2,3 In extravillous trophoblastic (HTR-8/SVneo) cells, LIF increases their invasiveness by activating STAT and ERK1/2-dependent signaling pathways. 2 Silencing of STAT3 expression in trophoblastic JEG-3 cells led to a decrease in LIF-mediated increase in invasiveness. 3 However, the role of another signaling pathway (ERK1/2) activated by LIF in the regulation of cellular invasion has not been studied in detail.…”