STAT3 and ERK1/2 Cross-talk in Leukaemia Inhibitory Factor Mediated Trophoblastic JEG-3 Cell Invasion and Expression of Mucin 1 and Fos

Suman, Pankaj; Gupta, Seema

doi:10.1111/aji.12248

Cited by 26 publications

(20 citation statements)

References 18 publications

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“…including Muc-1, through the janus kinase (JAK)/STAT3 and ERK1/2 signaling pathways (Suman & Gupta, 2014). Its suggested role in preventing implantation is consistent with its expression on the apical surface of the mouse LE, and its expression is timely downregulated before implantation (Surveyor et al, 1995).…”

Section: Discussionmentioning

confidence: 90%

“…LIF mRNA is expressed in both the endometrium and the blastocyst at the time of implantation, and its expression continues after the implantation for decidualization and the formation of the placenta (Nicola & Babon, ). Previous studies have also shown that in JEG‐3 cells, LIF induces changes in the expression of various genes involved in invasive cells, including Muc‐1, through the janus kinase (JAK)/STAT3 and ERK1/2 signaling pathways (Suman & Gupta, ). Its suggested role in preventing implantation is consistent with its expression on the apical surface of the mouse LE, and its expression is timely downregulated before implantation (Surveyor et al, ).…”

Section: Discussionmentioning

confidence: 99%

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Calcitonin administration improves endometrial receptivity via regulation of LIF, Muc‐1 and microRNA Let‐7a in mice

Shokrzadeh

Alivand

Abedelahi

et al. 2018

Journal Cellular Physiology

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Calcitonin (CT) is one of the factors affecting the embryo implantation, but its effects on the implantation window have not been fully investigated. The current study investigated the effects of CT on the endometrium receptivity by morphological study and evaluation of leukemia inhibitory factor (LIF), mucin 1 (Muc‐1), and microRNA (miRNA) Let‐7a in the ovarian stimulation and the normal ovarian cycle. Then the mechanism of the CT effects through the mammalian target of rapamycin (mTOR) signaling pathway was studied by using PP242. A total of 64 BALB/c mice were divided into the normal ovarian cycle and ovarian stimulation groups. Each group consisted of four subgroups: control, calcitonin, PP242, and calcitonin+PP242. CT and PP242 were injected on the fourth of pregnancy into the mice and 24 hr later all the mice were killed. The uterine tissue samples were used for morphological analysis, and endometrial cells were mechanically isolated for evaluation of gene and protein expression. The results showed that ovarian stimulation induced mTOR phosphorylation as well as increased expression of the Let‐7a miRNA. In addition, CT injection increased the expression of LIF and miRNA Let‐7a in ovarian stimulation similar to that in normal ovarian cycles. However, injection of PP242 reduced expression of miRNA Let‐7a and increased Muc‐1 expression in ovarian stimulation group. In conclusion, the administration of CT improved endometrial receptivity in mice. This phenomenon occurred by upregulation of LIF, miRNA Let‐7a and downregulation of Muc‐1 via mTOR signaling pathway.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Calcitonin administration improves endometrial receptivity via regulation of LIF, Muc‐1 and microRNA Let‐7a in mice

Shokrzadeh

Alivand

Abedelahi

et al. 2018

Journal Cellular Physiology

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“…Prolactin is perhaps best known for its role in lactation [32]; however, it is also responsible for activating several other pathways such as the JAK-STAT pathway, including STAT3 [33, 34]. The function of STAT3 is well established in pregnancy, including involvement with embryonic implantation [35, 36], angiogenesis [37], and parturition [38, 39]. …”

Section: Discussionmentioning

confidence: 99%

Evaluation of circulating miRNAs during late pregnancy in the mare

et al. 2017

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MicroRNAs (miRNAs) are small, non-coding RNAs which are produced throughout the body. Individual tissues tend to have a specific expression profile and excrete many of these miRNAs into circulation. These circulating miRNAs may be diagnostically valuable biomarkers for assessing the presence of disease while minimizing invasive testing. In women, numerous circulating miRNAs have been identified which change significantly during pregnancy-related complications (e.g. chorioamnionitis, eclampsia, recurrent pregnancy loss); however, no prior work has been done in this area in the horse. To identify pregnancy-specific miRNAs, we collected serial whole blood samples in pregnant mares at 8, 9, 10 m of gestation and post-partum, as well as from non-pregnant (diestrous) mares. In total, we evaluated a panel of 178 miRNAs using qPCR, eventually identifying five miRNAs of interest. One miRNA (miR-374b) was differentially regulated through late gestation and four miRNAs (miR-454, miR-133b, miR-486-5p and miR-204b) were differentially regulated between the pregnant and non-pregnant samples. We were able to identify putative targets for the differentially regulated miRNAs using two separate target prediction programs, miRDB and Ingenuity Pathway Analysis. The targets for the miRNAs differentially regulated during pregnancy were predicted to be involved in signaling pathways such as the STAT3 pathway and PI3/AKT signaling pathway, as well as more endocrine-based pathways, including the GnRH, prolactin and insulin signaling pathways. In summary, this study provides novel information about the changes occurring in circulating miRNAs during normal pregnancy, as well as attempting to predict the biological effects induced by these miRNAs.

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“…The role of gp130 cytokines in trophoblast cells has been most extensively reviewed elsewhere and hence only briefly discussed here. IL‐6 and LIF stimulate the invasion of primary trophoblast and JEG‐3 choriocarcinoma cells; the involvement of IL‐11 is less clear. IL‐11 inhibits the invasion of primary trophoblast and HTR‐8/SVneo cells, but increases the invasion of the choriocarcinoma JEG‐3 cells and stimualtes migration of EVTs …”

Section: Pro‐invasive Factorsmentioning

confidence: 99%

Decidual Control of Trophoblast Invasion

Sharma

Godbole

Modi

2016

American J Rep Immunol

234

184

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At the time of implantation, the trophoblast cells of the embryo adhere and then invade into the maternal endometrium and eventually establish placentation. The endometrium at the same time undergoes decidualization, which is essential for successful pregnancy. While the NK cells of the decidua have been implicated to play a key role in trophoblast invasion, few evidence are now available to demonstrate a pro-invasive property of decidual stromal cells. Secretions from decidualized endometrial stromal cells promote invasion of primary trophoblasts and model cell lines by activating proteases and altering expression of adhesion-related molecules. The decidual secretions contain high amounts of pro-invasive factors that include IL-1b, IL-5, IL-6, IL-7, IL-8, IL-9, IL-13, IL-15, Eotaxin CCL11, IP-10 and RANTES, and anti-invasive factors IL-10, IL-12 and VEGF. It appears that these decidual factors promote invasion by regulating the protease pathways and integrin expression utilizing the STAT pathways in the trophoblast cells. At the same time the decidua also seem to secrete some anti-invasive factors that are antagonist to the matrix metalloproteinases and/or are activators of tissue inhibitors of metalloproteinases. This might be essential to neutralize the effects of the invasionpromoting factors and restrain overinvasion. It is tempting to propose that during the course of pregnancy, the decidua must balance the production of these pro and anti-invasive molecules and such harmonizing production would allow a timely and regulated invasion.

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STAT3 and ERK1/2 Cross-talk in Leukaemia Inhibitory Factor Mediated Trophoblastic JEG-3 Cell Invasion and Expression of Mucin 1 and Fos

Abstract: ERK1/2 as well as JAK-STAT-mediated STAT3 (Ser727) phosphorylation play an important role in LIF-mediated JEG-3 trophoblastic cell invasion and gene expression.

Cited by 26 publications

References 18 publications

Calcitonin administration improves endometrial receptivity via regulation of LIF, Muc‐1 and microRNA Let‐7a in mice

Calcitonin administration improves endometrial receptivity via regulation of LIF, Muc‐1 and microRNA Let‐7a in mice

Evaluation of circulating miRNAs during late pregnancy in the mare

Decidual Control of Trophoblast Invasion

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