Leukocyte Adhesion to the Coronary Microvasculature During Ischemia and Reperfusion in an In Vivo Canine Model

Sheridan, Frank M.; Cole, Paul G.; Ramage, David

doi:10.1161/01.cir.93.10.1784

Cited by 87 publications

(43 citation statements)

References 25 publications

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“…Exuberant neutrophil adhesion in the microvasculature is implicated in a variety of inflammatory diseases ranging from arthritis to ischemia-reperfusion injury [27,28]. In SCD, elevated leukocyte counts correlate with greater risk of complications and mortality [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Inflammatory potential of neutrophils detected in sickle cell disease

et al. 2004

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An early event in the inflammatory response is neutrophil recruitment to endothelium in response to chemotactic stimulation, which in turn activates CD18-integrin, which anchors neutrophils to the vessel wall under the shear force of blood flow. Activated neutrophils circulating in sickle cell disease (SCD) patients may significantly contribute to vascular occlusions (VOC) as neutrophils adherent to inflamed endothelium recruit sickle red blood cells inducing VOC. To elucidate the mechanisms by which neutrophils may participate in VOC in SCD, CD18-integrin expression and function in fresh blood samples of non-crisis patients were measured by flow cytometry. CD11b/CD18 membrane expression was~70% higher on unstimulated SCD neutrophils than controls, which correlated with a 1-fold higher rate of adhesion to ligand. Unstimulated SCD neutrophils expressed~30,000 active CD18 per cell, while controls expressed~6,000. Stimulation with a low concentration of IL-8 (0.1 nM) upregulated 100% more active CD18 and induced 60% more adhesion of SCD than control neutrophils. These data demonstrate that neutrophils from SCD patients constitutively express active CD18 in blood and respond with enhanced sensitivity to chemokine activation of adhesion, thus increasing their propensity for exuberant adhesion. Am.

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Section: Discussionmentioning

confidence: 99%

Inflammatory potential of neutrophils detected in sickle cell disease

et al. 2004

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“…Adhesion of platelets to circulating myeloid leukocytes in vitro and in vivo is related to platelet activation (26). It is known that reperfusion of the ischemic myocardium often leads to rapid accumulation and adhesion of leukocytes to the endothelium, which are supposed to be the initial steps of leukocyte-mediated reperfusion injury (27,28). We might speculate that patients with a higher MPV have a higher potential for platelet-leukocyte interaction and thus, are more prone to platelet-activated, leukocyte-mediated reperfusion injury.…”

Section: Myocardial Infarction; Mpv Mean Platelet Volume; Wbc Whitmentioning

confidence: 99%

Relationship of admission hematological indexes with myocardial reperfusion abnormalities in acute ST segment elevation myocardial infarction patients treated with primary percutaneous coronary interventions

Maden¹,

Kacmaz²,

Selçuk³

et al. 2009

Canadian Journal of Cardiology

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“…Although there was no difference in no-reflow zones between the neutropenic animals and the control group in a gerbil cerebral ischemia model, 36,37 other studies showed that reperfusion leads to rapid accumulation of leukocytes in the microvasculature of the dog heart. 38 This may be mediated by CD18-dependent leukocyte adhesion 39 and may play some role in the genesis of the no-reflow phenomenon. Byrne et al 40 found that reperfusion with leukocyte-depleted blood may reduce cardiac no reflow.…”

Section: Pathophysiologymentioning

confidence: 99%