Leukocytes Cultured from Small Inocula of Whole Blood and the Preparation of Metaphase Chromosomes by Treatment with Hypotonic KCL

Hungerford, David A.

doi:10.3109/10520296509116440

Cited by 534 publications

(143 citation statements)

References 9 publications

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“…The cells were removed from flasks by trypsinization and processed through hypotonic KCI and fixative (Hungerford, 1965 human breast-cell lines (McGrath et al, 1974) and a canine mammary-cell line (Watrach et al, 1978). Thus to check for the presence of retroviruses in this canine mammary-cell line, labelling with [3H]-uridine was carried out at passage 120, followed by concentration of the culture supernatant and sucrose density centrifugation as outlined by Norval & Marmion (1976).…”

mentioning

confidence: 99%

The characteristics of a canine mammary carcinoma cell line, REM 134

Else¹,

Norval²,

Neill³

1982

Br J Cancer

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mentioning

confidence: 99%

The characteristics of a canine mammary carcinoma cell line, REM 134

Else¹,

Norval²,

Neill³

1982

Br J Cancer

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“…Low plasma levels of vitamin C were found in all groups from uranium districts including controls (physiological range between 5 and 15 mg/L). Plasma levels ofvitamin B,2 were also low in the mining districts compared to Prague.…”

Section: Resultsmentioning

confidence: 99%

Monitoring Genotoxic Exposure in Uranium Miners

Šrám¹,

Binková²,

Dobiáš³

et al. 1993

Environmental Health Perspectives

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Recent data from deep uranium mines in Czechoslovakia indicated that in addition to radon daughter products, miners are also exposed to chemical mutagens. Mycotordns were identified as a possible source of mutagenicity present in the mines. Various methods of biomonitoring were used to eaamine three groups of miners from different uranium mies. Cytogenetic analysis of peripheral lymphocytes, unscheduled DNA synthesis (UDS) in lymphocytes, and lipid peroxidation (LPO) in both plasma and lymphocytes were studied on 66 exposed miners and 56 cotrols. Throat swabs were taken frm 116 miners and 78 controls. Significantly increased numbers of aberrant cells were found in all groupsof miners, as well as decreased UDS values in lymphocytes and increased LPO plasma levels in comparison to controls. Molds were detected in throat swabs from 27% of miners, and 58% ofthese molds were embryotoxic. Only 5% of the control samples contained molds and none of them was embryotoxic. The following mycotoxins were isolated from miners' throat swab samples: rugulosin, sterigmatocystin, mycophenolic acid, brevianamid A, citreoviridin, citrinin, penicilic acid, and secalonic acid. These data suggest that mycotoxins are a genotoxic factor affecting uranium miners. Introduc#onUranium ore in Czechoslovakia is mined using very deep mines (1200-1600 m), which have warm temperatures and high humidity. The risk of occupational exposure in such mines has been related to the carcinogenic effect of radon daughter products, inducing lung cancer (1). To reduce this exposure to radon, ventilation in these mines was increased substantially in the period [1968][1969][1970][1971][1972]. As a result of this improvement the radon exposures in these mines are 1.0-1.5 x 10"°MeV per year and infrequently are as high as 3.0-4.0 x 10"' MeV (limit is 8 x 1010 MeV/year). In spite of the decreased exposure to radionuclides, lung cancer incidence among these uranium miners has not decreased.In 1970, Kusak et al. (2) put forward the hypothesis that radionuclides may be accumulated in molds growing in uranium mines. Because these molds also produce aflatoxin, we initially looked for evidence of aflatoxin exposure by using an assay for measuring aflatoxin-albumin adducts (3). However, in a pilot study on 20 blood samples, no increased adduct levels were 'Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Czechoslovak Academy of Sciences, 120 00 Prague, Czechoslovakia. observed (C. P. Wild, unpublished data). Samples from walls and woods in the uranium mines contained molds. Altogether, 90 types of molds were isolated, and 72 % ofthem were embryotoxic (genus Aspergillus, Penicillium, Fusarium) and mutagenic using the Ames test and SOS chromotest (4). From these samples we isolated the mycotoxins sterigmatocystin, mycophenolic acid, brevianamid A, citreoviridin, penicilic acid, aflatoxin BI and GI, rugulosin, and citrinin. Therefore, we hypothesized that these mutagenic mycotoxins may be another risk factor responsible for the continued high cance...

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“…Testes were dissected out in normal saline and pretreated for about an hour at room temperature with hypotonic solutions (TJIO and WHANG 1962) such as potassium chloride (KCI) (HUNGERFORD 1965) and sodium citrate (Na citrate) at varying molarities. The use of KCl at 0.125 to 0.150M yeilded fairly good results in the preparation of meiotic prophase chromosomes.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Male Meiosis in Five Species of Indian Diplopoda (Myriapoda)

Achar

1984

Caryologia

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SUMMARY -A sequential study of male meiosis of five species of south Indian Diplopoda (Myriapoda), namely, Cingalobolus sp., Trigoniulus sp., (Family Trigoniulidae), Carlogonus palmatus, Thyropygus sp., and Thyropygus alienus (Family Harpagophoridae) has been made by the use of air drying technique. The pretreatment of mitotic and meiotic tissues with colchicine (0.05%) and hypotonic Na citrate (0.016-0.021M) and KCl (0.125-0.150M) has enabled to secure well-spread mitotic and meiotic chromosomes, which, otherwise were found to be cytologically difficult to obtain. Some significant cytological features, such as heteropycnosis, premetaphase stretching of chromosomes, nonstaining gaps in the chromosomal bivalents and ring formation by the sex-chromosomes (XY) have been discussed.

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Leukocytes Cultured from Small Inocula of Whole Blood and the Preparation of Metaphase Chromosomes by Treatment with Hypotonic KCL

Cited by 534 publications

References 9 publications

The characteristics of a canine mammary carcinoma cell line, REM 134

The characteristics of a canine mammary carcinoma cell line, REM 134

Monitoring Genotoxic Exposure in Uranium Miners

Analysis of Male Meiosis in Five Species of Indian Diplopoda (Myriapoda)

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