Leukocytes from Patients Allergic to Chromium and Nickel Examined by the Sealed Capillary Migration Technique

Abstract

Leukocytes from patients allergic to chromium and nickel were studied by a sealed capillary migration test. The migration indices were determined at 2, 5 and 24 h. The chromium-allergic group could be differentiated from the control group at all investigated times, and especially at 2 and 5 h, by using a potassium dichromate concentration of 1.7 × 10^––5M in the capillaries. The migration indices in the nickel-allergic group could not be used for discrimination from the controls. The highest chromate (1.1 × 10^––4M) and nickel sulfate (3.8 × 10^––4M) concentrations inhibited the migration of leukocytes from both patients and controls, indicating toxic effects.

Protecting Effects of Vasoactive Intestinal Polypeptide on Lymphocytes Against Metal Toxicity

“…It was dissolved in 0.9% saline and used in the lymphocyte cultures at a final concentration of 3.8~10' mol/l, which has previously been found to give an inhibiting effect on cellular DNA synthesis (14) and migration (15).…”

“…However, in higher doses it may give a toxic skin reaction. In vitro, nickel sulfate may intluence different lymphocyte functions such as DNA synthesis (14) and migraticm (15) in a dose-dependent way. High doses are inhibitory, probably by direct effect on the nucleus, which has been shown with, e.g., Chinese hamster ovary cells (16).…”