Protecting Effects of Vasoactive Intestinal Polypeptide on Lymphocytes Against Metal Toxicity

Nordlind, Klas; Bondesson, L.; Agerberth, Birgitta; Mutt, Viktor

doi:10.3109/08923979209009227

Cited by 3 publications

(1 citation statement)

References 17 publications

(2 reference statements)

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“…It has also shown protective e¡ects on lymphocytes against metal toxicity (18). Bandypadhyay et al (19) concluded that VIP may play a signi¢cant protective role in the gastrointestinal tract against the damaging e¡ects of nitric oxide induced by cytotoxic agents.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Vasoactive Intestinal Peptide on the Challenge Phase of Allergic Contact Dermatitis in Humans

Lundeberg

Mutt²,

Nordlind³

1999

Acta Dermato-Venereologica

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There is increasing evidence that the nervous system has influence on the immune response. The effect of vasoactive intestinal peptide (VIP) and of serotonin and its antagonists on the challenge phase of allergic contact dermatitis in humans were tested. The substances were injected intracutaneously shortly before and 6 h after application of patch tests with nickel sulphate in nickel-allergic patients and the test areas were measured after a further 18 h. Biopsy specimens were also taken for immunohistochemistry. The diameter of the nickel sulphate-induced test reaction was significantly reduced after injection of VIP at 10(-6)-10(-5) mol/l, but was not affected by serotonin or ketanserin. Also tested was the influence of the substances on the response of peripheral blood mononuclear cells from nickel-allergic subjects to nickel sulphate, when added at the same time as the antigen. No effect on the cell proliferative rate was seen, except for an inhibitory effect of serotonin and its antagonists at 10(-5)-10(-4) mol/l. VIP, at 10(-5) mol/l and serotonin at 10(-4) mol/l stimulated the secretion of interferon gamma. The interleukin-2 soluble receptor secretion was slightly stimulated by 5-HT at 10(-4) mol/l and by ketanserin at 10(-6) mol/l. In conclusion, our results show that when injected intracutaneously in the challenge phase of allergic contact dermatitis, VIP has an inhibitory effect, which might be explained by enhanced leukocyte production of interferon gamma.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Vasoactive Intestinal Peptide on the Challenge Phase of Allergic Contact Dermatitis in Humans

Lundeberg

Mutt²,

Nordlind³

1999

Acta Dermato-Venereologica

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Glutamate- and aspartate-like immunoreactivities in human normal and inflamed skin

Nordlind

Johansson

Lidén

et al. 1993

Virchows Archiv B Cell Pathol

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The Effect of Nickel Compounds on Mitogen Dependent Human Lymphocyte Stimulation

Sikora¹,

Zeromski²

1995

Int J Immunopathol Pharmacol

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The effect of metal salts, three nickel and one non-nickel (manganese chloride), was examined on the ability to influence mitogen stimulated normal human blood lymphocytes by means of short term in vitro culture and a tritiated thymidine incorporation test. Purified lymphocytes were incubated for one hour with tissue culture medium containing either one of the nickel salts tested (nickel subsulfide, nickel sulfate or nickel acetate) or manganese chloride. Separate portions of cells were incubated in the metal salt mixtures containing both, nickel and manganese salts. All metal salts were used in predetermined subtoxic concentrations. Two mitogens, phytohemagglutinin (PHA) and concanavalin A (Con A), were used as lymphocyte stimulatory agents. Cells were cultured for 72 hrs. It was found that following incubation with nickel salts, mitogen dependent lymphocyte stimulation was inhibited proportionally to the metal salt concentration. This blocking effect on tritiated thymidine incorporation was stronger for readily soluble nickel salts i.e. sulfate and acetate than for almost insoluble nickel subsulfide with either mitogen used. Manganese chloride used as a single salt resulted in a dose-dependent increase of lymphocyte stimulation as compared to the mitogen stimulated cells without preincubation with either metal (control samples). Cells preincubated with nickel salt-manganese chloride mixtures exhibited an increase of thymidine incorporation but below values for control cells.

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Protecting Effects of Vasoactive Intestinal Polypeptide on Lymphocytes Against Metal Toxicity

Cited by 3 publications

References 17 publications

Inhibitory Effect of Vasoactive Intestinal Peptide on the Challenge Phase of Allergic Contact Dermatitis in Humans

Inhibitory Effect of Vasoactive Intestinal Peptide on the Challenge Phase of Allergic Contact Dermatitis in Humans

Glutamate- and aspartate-like immunoreactivities in human normal and inflamed skin

The Effect of Nickel Compounds on Mitogen Dependent Human Lymphocyte Stimulation

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