Leukotriene B4 in synovial fluid

Davidson, E. M.; Rae, S.; Smith, M. J. H.

doi:10.1111/j.2042-7158.1982.tb04745.x

Cited by 51 publications

(12 citation statements)

References 5 publications

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“…However, the groups were not matched for age, sex, or background drug therapy. LTB4 has been detected in the synovial fluid of RA patients (27,28), and reduced chemotactic sensitivity of peripheral blood neutrophils has been noted in RA patients (29). Furthermore, preincubation of normal neutrophils with chemotactic factors results in specific desensitization of the neutrophils to the chemotactic factor (30)(31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary supplementation with marine fish oil on leukocyte lipid mediator generation and function in rheumatoid arthritis

Weinblatt

et al. 1987

Arthritis & Rheumatism

197

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tion, the ratio of arachidonic acid to eicosapentaenoic acid in the patients' neutrophil cellular lipids decreased from 81:l to 2.7:1, and the mean generation of leukotriene B4 (with calcium ionophore stimulation) significantly declined by 33%. The mean neutrophil chemotaxis to both leukotriene B4 and FMLP significantly increased toward the normal range at week 6. The generation of 5-lipoxygenase products by calcium ionophorstimulated monocytes was not significantly suppressed, but a significant decline (37 %) in plateletactivating factor generation was noted at week 6. The modulation of these measures of leukocyte inflammatory potential suggests that fish oil supplementation may have an antiinflammatory effect.Dietary supplementation with fish oil fatty acids, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) in healthy volunteers has been shown to increase the relative content of EPA in leukocyte membranes and to decrease the response of the leukocytes to both transmembrane and calcium ionophore activation (1). The chemotactic dosedependent response of neutrophils to leukotriene B4 (LTB4) was significantly suppressed at 6 weeks of supplementation, and the chemotactic sensitivity was restored when the supplement was discontinued. The 5-lipoxygenation of arachidonic acid in response to activation with calcium ionophore was inhibited at 2 points. Fatty acid hydrolase function was attenuated, as defined by a reduced release of labeled arachidonic acid from prelabeled neutrophils and monocytes. Function of the 5-lipoxygenase enzyme (Figure 1) was suppressed, as determined by reduced generation of the sequential arachidonic acid-derived reaction products, 5-hydroperoxyeicosatetraenoic acid (measured

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Section: Discussionmentioning

confidence: 99%

Effects of dietary supplementation with marine fish oil on leukocyte lipid mediator generation and function in rheumatoid arthritis

Weinblatt

et al. 1987

Arthritis & Rheumatism

197

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“…Finally, it has been shown to exert antiapoptotic activity on neutrophils (10). LTB 4 has been detected in significant concentrations in various inflammatory conditions, including inflammatory synovial exudates (11,12), psoriatic skin lesions (13,14), and sputum from chronic obstructive pulmonary disease (15). LTB 4 has also been shown to play a role in experimental models of asthma (16), ulcerative colitis (17), and postischemic tissue injury (18,19).…”

Section: Eukotriene B 4 (Ltb 4 )mentioning

confidence: 99%

Modulation of Leukotriene B4 Receptor-1 Expression by Dexamethasone: Potential Mechanism for Enhanced Neutrophil Survival

Staňková

Turcotte

Harris

et al. 2002

The Journal of Immunology

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Glucocorticoids can down-regulate many inflammatory and immune responses and constitute a powerful therapeutic tool in a number of diseases. However, they have a somewhat paradoxical effect on neutrophils, in that they prolong their survival. Because leukotriene B4 (LTB4) can also extend neutrophil survival, we proposed that glucocorticoids could prevent neutrophil apoptosis by up-regulating their expression of the high-affinity LTB4 receptor (BLT1). Here we show that, indeed, dexamethasone (DEX) up-regulates the steady-state levels of BLT1 mRNA in human neutrophils. The effect was time and concentration dependent, being maximal at 4 h and at 10–100 nM DEX. The effect was also dependent on transcriptional activity, whereas BLT1 mRNA stability was not affected. DEX-induced up-regulation of BLT1 expression was prevented by pretreatment with the LTB4 antagonist LY255283. Moreover, LTB4 itself up-regulated the expression of BLT1 mRNA. BLT1 protein expression on neutrophils exposed to DEX for 24 h was also up-regulated 2- to 3-fold, and DEX-treated as well as LTB4-treated cells showed enhanced responsiveness to LTB4 in terms of intracellular Ca2+ mobilization and chemotaxis. Whereas DEX and LTB4 alone decreased neutrophil apoptosis by ∼50%, neutrophils treated with both LTB4 and DEX showed >90% survival at 24 h. Moreover, BLT1 antagonists prevented the increased neutrophil survival induced by DEX as well as by LTB4. Taken together, our results suggest that DEX-induced up-regulation of BLT1 expression in neutrophils may be one mechanism through which glucocorticoids can prolong neutrophil survival, namely by enhancing cell responses to the antiapoptotic effect of LTB4.

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“…Thus, LTB4 may serve as an intracellular second messenger in the receptors for the chemotactic factors described above. However, no report has compared the LTB, production in neonatal PMN with that of adult PMN, partly because of the great difficulty of separating LTB4 from its stereospecific isomers, and partly because of the great difficulty in measuring LTB4 at the low concentrations present in biological fluids (7).…”

mentioning

confidence: 99%

“…And LTB, is reported to exist in the synovial fluid of patients with rheumatoid arthritis and gout, and in the skin of patients with atopic dermatitis (7)(8)(9). PMN not only show chemotaxis when exposed to LTB4 but they also produce LTB4 when stimulated by chemotactic factors such as C5a, fMLP, PAF, and by the calcium ionophore A23187 (10)(11)(12)(13)(14).…”

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confidence: 99%

Leukotriene B4 Biosynthesis in Polymorphonuclear Leukocytes from Blood of Umbilical Cord, Infants, Children, and Adults

1986

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ABSTRACT. The biosynthesis of 5(S), 12(R)-dihydroxy-6,14-cis-8,lO-trans-eicosatetraenoic acid, leukotriene B4, by human polymorphonuclear leukocytes was examined in relation to age. The leukotriene B4 production by polymorphonuclear leukocytes from unbilical cords, infants, and adults was assayed using high pressure liquid chromatography. The specificity of the leukotriene B4 assay was examined by gas chromatography mass spectrometry. Polymorphonuclear leukocytes from 10 umbilical cords, 24 infants and children, and 10 adults were examined for their ability to synthesize leukotriene B4, in vitro after stimulation by the ionophore A23187 or platelet-activating factor. Among the infants and children, there was a slight agedependent increase of leukotriene B4 production by polymorphonuclear leukocytes in response to ionophore A23187, but it was not statistically significant. Leukotriene B4 production by polymorphonuclear leukocytes in the umbilical cords and infants was not significantly lower than that of polymorphonuclear leukocytes in adults in response to both ionophore A23187 and platelet-activating factor under our experimental conditions. (Pediatr Res 20: 402-406,1986) Abbreviations PMN, polymorphonuclear leukocytes LTB4, leukotriene B4; 5(S),12(R)-dihydroxy-6,14-cis-8,10-trans-eicosatetraenoic acid GC-MS, gas chromatography mass spectrometry PAF, platelet-activating factor fMLP, formyl-methionyl-leucyl-phenylalanine RP-HPLC, reversed phase high pressure liquid chromatography SP-HPLC, straight phase high pressure liquid chromatography RIA, radioimmunoassay SIM, selected ion monitoring 5S,12S-DHETE, 5(S),12(S)-dihydroxy-6,10-trans-8,14-cis-eicosatetraenoic acid PGB, prostaglandin B2 [2H4]PGF2,, tetradeuterated prostaglandin Fza ME-DMiPS, methyl ester dimethylisopropylsilyl

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Leukotriene B4 in synovial fluid

Cited by 51 publications

References 5 publications

Effects of dietary supplementation with marine fish oil on leukocyte lipid mediator generation and function in rheumatoid arthritis

Effects of dietary supplementation with marine fish oil on leukocyte lipid mediator generation and function in rheumatoid arthritis

Modulation of Leukotriene B4 Receptor-1 Expression by Dexamethasone: Potential Mechanism for Enhanced Neutrophil Survival

Leukotriene B4 Biosynthesis in Polymorphonuclear Leukocytes from Blood of Umbilical Cord, Infants, Children, and Adults

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