LEUNIG_HOMOLOG Mediates MYC2-Dependent Transcriptional Activation in Cooperation with the Coactivators HAC1 and MED25

You, Yanrong; Zhai, Qingzhe; An, Chunpeng; Li, Chuanyou

doi:10.1105/tpc.19.00115

Cited by 60 publications

(65 citation statements)

References 87 publications

(155 reference statements)

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“…We reasoned that, as an effective coactivator of MYC2 and other JA-inducible transcription factors (Çevik et al, 2012;Chen et al, 2012;An et al, 2017), MED25 must have evolved sophisticated mechanisms to reconcile and coordinate these opposing functions. To explore these mechanisms, we performed an affinity purification-coupled mass spectrometry assay with MED25-myc plants (Chen et al, 2012) to identify MED25associated proteins (You et al, 2019). In this assay, PRP39a and PRP40a, together with two other RNA processing proteins, were identified as MED25-associated proteins (Supplemental Table).…”

Section: Splicing Factors Prp39a and Prp40a Associate With Med25mentioning

confidence: 99%

“…Upon hormone elicitation, MED25 physically cooperates with the epigenetic regulator HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY1, which selectively regulates hormoneinduced histone-3 Lys-9 acetylation of MYC2 target promoters (An et al, 2017). At the same time, MED25 also physically recruits the conserved transcriptional coregulator LEUNIG_HOMOLOG, which acts as a scaffold to stabilize the MYC2-MED25-HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY1 transcription complex (You et al, 2019). Clearly, these aspects of functions of MED25 all favor the activation of MYC2-dependent JA responses.…”

Section: Med25 Acts As a Central Coactivator Of Myc2-mediated Transcrmentioning

confidence: 99%

“…We have shown that the plant Mediator subunit MED25 physically and functionally interacts with MYC2, thereby playing a pivotal role in PIC formation during the activation of MYC2mediated transcription of JA-responsive genes (Chen et al, 2012). Furthermore, MED25 is also involved in the assembly of a MYC2-MED25 functional transcription complex, which acts as an integrative hub to coordinate the actions of multiple regulators during hormone-triggered activation of MYC2 (An et al, 2017;Du et al, 2017;Liu et al, 2019;Wang et al, 2019;You et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Mediator Subunit MED25 Couples Alternative Splicing of JAZ Genes with Fine-Tuning of Jasmonate Signaling

Deng

Zhai

et al. 2019

Plant Cell

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JASMONATE ZIM-DOMAIN (JAZ) transcriptional repressors are key regulators of jasmonate (JA) signaling in plants. At the resting stage, the C-terminal Jas motifs of JAZ proteins bind the transcription factor MYC2 to repress JA signaling. Upon hormone elicitation, the Jas motif binds the hormone receptor CORONATINE INSENSITIVE1, which mediates proteasomal degradation of JAZs and thereby allowing the Mediator subunit MED25 to activate MYC2. Subsequently, plants desensitize JA signaling by feedback generation of dominant JAZ splice variants that repress MYC2. Here we report the mechanistic function of Arabidopsis (Arabidopsis thaliana) MED25 in regulating the alternative splicing of JAZ genes through recruiting the splicing factors PRE-mRNA-PROCESSING PROTEIN 39a (PRP39a) and PRP40a. We demonstrate that JA-induced generation of JAZ splice variants depends on MED25 and that MED25 recruits PRP39a and PRP40a to promote the full splicing of JAZ genes. Therefore, MED25 forms a module with PRP39a and PRP40a to prevent excessive desensitization of JA signaling mediated by JAZ splice variants.

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Section: Splicing Factors Prp39a and Prp40a Associate With Med25mentioning

confidence: 99%

Section: Med25 Acts As a Central Coactivator Of Myc2-mediated Transcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mediator Subunit MED25 Couples Alternative Splicing of JAZ Genes with Fine-Tuning of Jasmonate Signaling

Deng

Zhai

et al. 2019

Plant Cell

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“…How TPR1 binding to promoters influences gene transcription and the chromatin environment needs to be clarified. It is remarkable that Gro/Tup1 proteins such as Arabidopsis Leunig homolog are also associated with gene expression activation (Chen and Courey, 2000; You et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Genome-wide chromatin binding of transcriptional corepressor Topless-related 1 inArabidopsis

Griebel

Lapin

Kracher

et al. 2020

Preprint

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1 Timely and specific regulation of gene expression is critical for plant responses to 2 environmental and developmental cues. Transcriptional coregulators have emerged 3 as important factors in gene expression control, although they lack DNA-binding 4 domains and the mechanisms by which they are recruited to and function at the 5 chromatin are poorly understood. Plant Topless-related 1 (TPR1), belonging to a 6 family of transcriptional corepressors found across eukaryotes, contributes to 7 immunity signaling in Arabidopsis thaliana and wild tobacco. We performed 8 chromatin immunoprecipitation and sequencing (ChIP-seq) on an Arabidopsis TPR1-9 GFP expressing transgenic line to characterize genome-wide TPR1-chromatin 10 associations. The analysis revealed ~1400 genes bound by TPR1, with the majority 11 of binding sites located at gene upstream regions. Among the TPR1 bound genes, 12 we find not only regulators of immunity but also genes controlling growth and 13 development. To support further analysis of TPR1-chromatin complexes and other 14 transcriptional corepressors in plants, we provide two ways to access the processed 15 ChIP-seq data and enable their broader use by the research community.16 17 Plant materials and growth conditions 121 Stable transgenic lines pTPR1:TPR1-GFP Col-0 (TPR-GFP) and pTPR1:TPR1-HA 122 #3 Col-0 (TPR1-HA) were described previously (Zhu et al., 2010). Plants were grown 123 on soil with a daily 10 h light period (150-200 µE/m 2 s) at 22 °C and 60 % relative 124 humidity. Plant leaf material was harvested after 6 weeks of growth. 125 126 Chromatin immunoprecipitation assays and sequencing (ChIP-seq) 127 ChIP was performed on 2 g leaf tissues of 6-week-old plants according to Gendrel et 128 al. (2005) with modifications (Birkenbihl et al., 2012). Leaf samples were cross-linked 129 twice by infiltration with 1% formaldehyde under a vacuum for 2x 7.5 minutes. Nuclei 130 were disrupted by sonication 10 times for 30 s each and 30 s of cooling in a 131 Bioruptor (Diagenode). IPs were performed using α-GFP (Ab6556, Abcam) 132 antibodies and Protein A agarose beads (Sigma). After elution of immune 133 complexes, cross-linking was reversed with an overnight incubation at 65°C in 134 200 mM NaCl followed by proteinase K digestion. DNA was extracted with phenol-135 chloroform and precipitated with ethanol in the presence of 0.3 M sodium acetate 136 (pH=5.2) and 10 µg/mL glycogen (overnight at -20°C). After centrifugation, DNA 137 pellets were washed with 70% ethanol, air-dried and resuspended in water before 138 PCR. MiniElute PCR Purification Kit (Qiagen, Germany) was used for cleaning ChIP 139 DNA and two rounds of in vitro transcription by T7 RNA polymerase followed 140 according to a linear DNA amplification (LinDA) protocol (Shankaranarayanan et al.

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“…Arabidopsis thaliana is a host plant of Spodoptera exigua, and is also a classic model plant to study plant resistance to herbivores. Here, we describe a method adapted from our previous work (You et al, 2019) to conduct the feeding assays with Spodoptera exigua larvae on Arabidopsis thaliana rosette leaves. By counting the weight of the larvae after feeding leaves from different genotypes, we were able to evaluate plant resistance to herbivore attacks in the laboratory settings.…”

Section: Feeding Assaymentioning

confidence: 99%

Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana

You¹,

An²,

Li³

2020

BIO-PROTOCOL

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Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model host plant Arabidopsis thaliana, which is simple and easy to conduct, and can be used to evaluate the effect of host genes on insect growth and thus to study plant resistance to chewing insects.

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LEUNIG_HOMOLOG Mediates MYC2-Dependent Transcriptional Activation in Cooperation with the Coactivators HAC1 and MED25

Cited by 60 publications

References 87 publications

Mediator Subunit MED25 Couples Alternative Splicing of JAZ Genes with Fine-Tuning of Jasmonate Signaling

Mediator Subunit MED25 Couples Alternative Splicing of JAZ Genes with Fine-Tuning of Jasmonate Signaling

Genome-wide chromatin binding of transcriptional corepressor Topless-related 1 inArabidopsis

Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana

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