Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress

Queiroz, Eveline Aparecida Isquierdo Fonseca de; Fortes, Zuleica Bruno; Cunha, Mário Antônio Alves da; Sarılmışer, Hande Kazak; Dekker, Aneli M. Barbosa; Öner, Ebru Toksoy; Dekker, Robert F. H.; Khaper, Neelam

doi:10.1016/j.ijbiomac.2017.04.035

Cited by 48 publications

(21 citation statements)

References 42 publications

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“…Inhibit tumor growth, cell cycle arrest, apoptosis and metastasis arrest (Wu et al, 2017) Levan Increase oxidative stress and apoptosis (Queiroz et al, 2017) L-asparaginase Causes nutritional de ciencies and inhibit protein synthesis resulting in apoptosis (Shrivastava et al, 2016) Ieodoglucomide B Inhibit tumor cell growth (Tareq et al, 2013) Bacillistatins 1 and 2 Inhibit tumor cell growth (Pettit, Arce, Chapuis, & Macdonald, 2015) Mixirins A, B and C Inhibit tumor cell growth (Harris & Pierpoint, 2012) Parasporin 1 Activates apoptotic signaling pathway by binding with Beclin 1 receptor and increase Ca 2+ in ux (Katayama et al, 2007) Parasporin 2 Permeabilize plasma membrane through GPIanchored protein (Abe et al, 2017) Parasporin 3 Pore formation (Ito et al, 2004) Parasporin 4 Cholesterol independent pore formation (Yamashita et al, 2005) Parasporin 6 Swelling of cells and vacuoles formation…”

Section: Surfactinmentioning

confidence: 99%

Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules

Ferdous¹,

Shishir²,

Khan³

et al. 2018

Microb. Bioacts.

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Cancer treatment remains as an expensive process due to the cost of sophisticated infrastructure development as well as its maintenance with skilled personnel. At the same time, the success rate is not very inspiring since non-specific target oriented medication could cause other health complexities leading to death. Research for alternative therapies aimed at minimizing the side effects of treatments and increasing the survival rates of patients includes routine explorations for anticancer agents from numerous sources (e.g. microbes, plants and nanoparticles). Anticancer activities of several bacterial components especially from Bacillus spp. were reported in many scientific reports. For economic production of these agents, potential strains from this genus could be feasible and sustainable for their long and successful utilization in industries. The review is therefore, focused on describing the available anticancer and anti-proliferative agents reported worldwide from Bacillus spp.

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Section: Surfactinmentioning

confidence: 99%

Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules

Ferdous¹,

Shishir²,

Khan³

et al. 2018

Microb. Bioacts.

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“…Recently, it was shown that the extract of Pseudoalteromonas mariniglutinosa collected from Erba and Nereus haloclines also inhibited the growth of MCF-7 cells [84]. Halomonas species can produce EPS which have been shown to have pro-apoptotic activity towards human T-leukemia cells and breast cancer MCF-7 cells [159,160]. Other bioactive extracts derived from Chromohalobacter salexigens, Chromohalobacter israelensis, Halomonas meridiana, and Idiomarina loihiensis have been found to be able to induce more than 70% mortality in HeLa cancer cells through different caspase-mediated apoptotic pathways [83].…”

Section: Marinementioning

confidence: 99%

Deep Hypersaline Anoxic Basins as Untapped Reservoir of Polyextremophilic Prokaryotes of Biotechnological Interest

2020

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Deep-sea hypersaline anoxic basins (DHABs) are considered to be among the most extreme ecosystems on our planet, allowing only the life of polyextremophilic organisms. DHABs’ prokaryotes exhibit extraordinary metabolic capabilities, representing a hot topic for microbiologists and biotechnologists. These are a source of enzymes and new secondary metabolites with valuable applications in different biotechnological fields. Here, we review the current knowledge on prokaryotic diversity in DHABs, highlighting the biotechnological applications of identified taxa and isolated species. The discovery of new species and molecules from these ecosystems is expanding our understanding of life limits and is expected to have a strong impact on biotechnological applications.

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“…Other bioactive molecules which have attracted much interest were biosurfactants of Halomonas sp., BS4 and levan (polysaccharide). These isolated molecules suppressed the cell viability in mammary epithelial carcinoma cell lines and human breast cancer MCF-7 cells [18,19]. To search for new antitumor compounds, a novel phenazine derivative together with six known compounds isolated from Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

“…This demonstrated the possibility of producing an eco-friendly drug from extremophilic organisms [18]. Furthermore, levan a (2→6)-d-fructan polysaccharide produced by Halomonas smyrnensis AAD6 T exhibited time- and concentration-dependent anticancer activity on human breast cancer MCF-7 cells [19]. Similarly, the cytotoxic effect of moderately halophilic bacterial bioactive compounds was observed against different tumor cells, namely the Bel 7402 [21] and HeLa S3 cell lines [16], human umbilical vein endothelial cells (HUVEC) and adipose-derived mesenchymal stem cells (MSCs) [22].…”

Section: Introductionmentioning

confidence: 99%

A Piscibacillus sp. Isolated from A Soda Lake Exhibits Anticancer Activity Against Breast Cancer MDA-MB-231 Cells

et al. 2019

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Microorganisms thrive in extreme environments and are known for synthesizing valuable metabolites. Salt-loving microorganisms can flourish in saline environments which inhibit the growth of other microbial life, and they possess the potential to produce stable and novel biomolecules for the use in biotechnological applications, including anticancer compounds. Sambhar Lake is the largest inland soda lake in India and is an appropriate habitat for halophilic bacterial and archaeal strains in terms of diversity and potential production of bioactive compounds. In the present study, a moderately halo-alkaliphilic bacterial strain C12A1 was isolated from Sambhar Lake, located in Rajasthan, India. C12A1 was gram-positive, motile, rod-shaped, formed oval endospores, produced carotenoids, and exhibited optimal growth at 37 °C in 10–15% NaCl (pH 8). C12A1 was found to be able to hydrolyze skimmed milk, gelatin, and Tween 80 but unable to hydrolyze starch and carboxymethylcellulose. C12A1 showed 98.87% and 98.50% identity in 16S rRNA gene sequence to P. halophilus and P. salipiscarius, respectively. Nevertheless, C12A1 was clustered within the clade consisting of P. salipiscarius strains, but it showed a distinct lineage. Thus, C12A1 was designated as Piscibacillus sp. Cell proliferation assay results showed that C12A1 broth extract (BEP) decreased cell viability in breast cancer MDA-MB-231 cells, which was confirmed by the MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Induction of cell toxicity was visualized by microscopy. Reverse Transcriptase PCR (RT-PCR) analysis demonstrated that BEP inhibited the expression of proliferative B-cell lymphoma-extra large (Bcl-xL) and cell cycle marker Cyclin-dependent kinase 2 (CDK2) at transcript levels. Similarly, cell migration and colony formation along with mesenchymal marker vimentin and stem cell marker BMI transcripts were found to be inhibited when cells were treated with the BEP. The anti-breast cancer potential of C12A1 indicates that microorganisms inhabiting saline-alkaline habitats, with Piscibacillus sp. in particular, are a promising source for discovery of novel bioactive substances.

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Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress

Cited by 48 publications

References 42 publications

Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules

Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules

Deep Hypersaline Anoxic Basins as Untapped Reservoir of Polyextremophilic Prokaryotes of Biotechnological Interest

A Piscibacillus sp. Isolated from A Soda Lake Exhibits Anticancer Activity Against Breast Cancer MDA-MB-231 Cells

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