Levels of Interleukin‐1β, ‐8, and ‐10 and RANTES in Gingival Crevicular Fluid and Cell Populations in Adult Periodontitis Patients and the Effect of Periodontal Treatment

Gamonal, Jorge; Acevedo, Agustín; Bascones, A; Jorge, O.; Silva, A.

doi:10.1902/jop.2000.71.10.1535

Cited by 314 publications

(328 citation statements)

References 58 publications

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“…Chemokines such as IL-8 and MCP-1 may be related to periodontitis severity (7,28). Previously, several investigators have shown that chemokine levels in gingival crevicular fluid decrease after periodontal therapy (7,12).…”

Section: Chemokinesmentioning

confidence: 99%

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Enhancement of gingival inflammation induced by synergism of IL-1^|^beta; and IL-6

et al. 2013

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Internleukin-1 (IL-1) and IL-6 are the most potent proinflammatory cytokines being involved in inflammatory diseases such as periodontitis. The objective of this study was to examine the synergistic effects of IL-1β and IL-6 on gingival inflammation by targeting cultured human gingival fibroblasts (HGFs). HGFs were treated with IL-1β or IL-6/soluble IL-6R (sIL-6R), and total RNA and total cell lysate were collected to examine expression of gp130 known as a signal transducer of IL-6 using qRT-PCR and Western blotting. IL-1β-mediated IL-6 productivity in HGFs was examined using ELISA method. Likewise, after HGFs and THP-1 macrophages were treated with IL-1β, TNF-α and IL-6, sIL-6R productivity was examined. Next, HGFs were treated with IL-6/ sIL-6R after pretreatment of IL-1β, and the intracellular signals were examined using Western blotting. Finally, various mRNA/protein expressions in HGFs treated with IL-6/sIL-6R after pretreatment of IL-1β were examined using qRT-PCR and ELISA method. IL-1β increased significantly both gp130 and IL-6 expression in HGFs. IL-6 increased significantly sIL-6R production in THP-1 macrophages but not HGFs. Co-stimulation with IL-1β and IL-6/sIL-6R induced dramatically the phosphorylation of Stat3, ERK and JNK in HGFs. Interestingly, expression of various inflammation-related molecules such as MMP-1, MCP-1, IL-1ra, bFGF and VEGF were enhanced by co-stimulation with IL-1β and IL-6/sIL-6R in HGFs. Gingival inflammation is regulated by HGFs affected by both IL-1β and IL-6/sIL-6R synergistically through induction of gp130 expression, resulting in progression of periodontitis.

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Section: Chemokinesmentioning

confidence: 99%

“…Previously, several investigators have shown that chemokine levels in gingival crevicular fluid decrease after periodontal therapy (7,12). Thus, chemokines seem to be attractive therapeutic targets for periodontitis management.…”

Section: Chemokinesmentioning

confidence: 99%

Enhancement of gingival inflammation induced by synergism of IL-1^|^beta; and IL-6

et al. 2013

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“…Gamonal et al (14,18) determinaron mayores niveles de CCL5 en sitios que presentaron actividad periodontal (47.02 pg/ 49.64 pg/µL) después de 2 meses de seguimiento comparados con sitios que no evidenciaron actividad en el mismo paciente (37.55 pg/47.53 pg/µL), datos que coinciden con el presente estudio para sitios periodontalmente comprometidos frente a sitios sanos. Sin embargo, los resultados discrepan con otros estudios (19)(20)(21) en cuanto a los niveles reportados para los sitios control, en los que se observó una cuantificación hasta 50% mayor en los niveles de CCL5.…”

Section: Discussionunclassified

“…Mientras los linfocitos Th1 son considerados la mayor fuente de RANK-L, molécula potencialmente inductora para la activación de los osteoclastos en las patologías periodontales, las células dendríticas y macrófagos son potenciales precursores de osteoclastos, reforzando el rol de ésta en la pérdida ósea (14,15). CCL5 se ha encontrado específicamente en tejidos periodontales de sujetos periodontalmente comprometidos, comparados con controles, y su concentración disminuye en el líquido crevicular después de aplicada la terapéutica periodontal.…”

Section: Introductionunclassified

Quantification of Chemokine CCL5 in Patients with Diabetes Mellitus Type 2 and/or Chronic Periodontitis: Preliminary Study

Sansores-España

Dds

Guzmán-Marín

et al. 2017

Odovtos - Int J Dent Sc

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RESUMENEl propósito del presente estudio fue cuantificar la presencia de la quimiocina CCL5 (RANTES) en Líquido Crevicular Gingival (LCG) de pacientes con Periodontitis Crónica (PC) y/o Diabetes Mellitus tipo 2 (DM2). Se realizó un estudio comparativo, transversal en 40 pacientes. Se tomó LCG de bolsas periodontales y surcos gingivales de 4 grupos de pacientes (10 por grupo de estudio), se excluyó a los pacientes que recibieron tratamiento periodontal, antibiótico y antiinflamatorio 6 meses anteriores al estudio o cursaron con alguna enfermedad sistémica distinta a DM2. Las concentraciones de CCL5 se determinaron mediante ensayos LUMINEX de selección magnética. Se realizó estadística descriptiva, prueba ANOVA de una vía, T de student y correlación de Pearson. La cuantificación de CCL5 fue mayor en los pacientes que presentaron ambas enfermedades, seguidos del grupo con solo PC, los sanos y el grupo con solo DM2. No se encontró diferencia significativa entre los grupos y no hubo correlación entre las cuantificaciones y los indicadores glicémicos. A pesar de que las diferencias no fueron significativas, el grupo de pacientes con ambas enfermedades presentó la mayor cuantificación de CCL5. La expresión de CCL5 en LGC debe considerarse un potencial inductor de destrucción periodontal, su determinación podría ser útil para monitoreo de la salud/enfermedad de los tejidos periodontales. SANSORES L., CARRILLO A., SAURI E., GUZMÁN E., HERNÁNDEZ M., POZOS A., MARTÍNEZ V., 2017: Cuantificación de la quimiocina CCL5 en pacientes con diabetes mellitus tipo 2 y/o periodontitis cronica: Estudio preliminar.-ODOVTOS-Int.

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“…IL-1 and TNF-α induce cells to produce CRP. In a number of studies, proinflammatory cytokines in gingival crevicular fluid and in inflamed gingival tissues were reported in a high amount at patients with active periodontal disease to the healthy group 6 . In another research, acute phase proteins, including CRP, has been found at increased levels in gingival crevicular fluid 7,8 .…”

Section: Introductionmentioning

confidence: 99%

The Relationship Between Periodontal Disease and Alzheimer’s Disease

Orbak¹,

Öztürk²

2016

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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Alzheimer's disease(AD) is a neurodegenerative disease that effects elder people. As the age increases, the prevalance of Alzheimer's disease also raises. As AD, periodontitis is seen more often in elder people. Periodontal disease has been associated with some systemical diseases such as cardiovascular diasease(CVS), diabetes mellitus, rheumatoid arthritis and preterm birth. Latterly, it has been investigated if there is a connection between Alzheimer's diasease. This review's aim is, inspecting if the periodontal disease contributes to Alzheimer's disease at literacy.

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Levels of Interleukin‐1β, ‐8, and ‐10 and RANTES in Gingival Crevicular Fluid and Cell Populations in Adult Periodontitis Patients and the Effect of Periodontal Treatment

Cited by 314 publications

References 58 publications

Enhancement of gingival inflammation induced by synergism of IL-1^|^beta; and IL-6

Enhancement of gingival inflammation induced by synergism of IL-1^|^beta; and IL-6

Quantification of Chemokine CCL5 in Patients with Diabetes Mellitus Type 2 and/or Chronic Periodontitis: Preliminary Study

The Relationship Between Periodontal Disease and Alzheimer’s Disease

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